Construction of highly-effective symbiotic bacteria: Evolutionary models and genetic approaches

Проворов, Н. А.; Онищук, О. П.; Yurgel, Svetlana N.; Курчак, О. Н.; Chizhevskaya, E. P.; Vorobyov, N. I.; Zatovskaya, T. V.; Симаров, Б. В.

doi:10.1134/s1022795414110118

Cited by 12 publications

(8 citation statements)

References 37 publications

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“…The initial stages of symbiogenesis can be addressed using the model of RLS, which demonstrates pronounced progressive evolution. From the plant side, it is manifested as the formation of specialized cellular and tissue structures in the nodules containing bacteria which form the joint C/N metabolic pathways with hosts (Provorov, Tikhonovich and Vorobyov, 2016). Progressive rhizobia evolution involves the emergence of deeply specialized cellular forms, terminally differentiated bacteroids, and a complicated architecture of genomestheir increased size and differentiation into several replicons such as circular or linear chromosomes, megaplasmids and chromids .…”

Section: Genetic Individuality In Symbiotic Microbesmentioning

confidence: 99%

Genetic individuality and interspecies altruism: modelling symbiogenesis using different types of symbiotic bacteria

Проворов

2021

BioComm

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In this minireview, we address the trade-off between biological altruism (group adaptation result-ing from the ability of an organism to improve the fitness of an associate at the expense of its own fitness) and symbiogenesis — the evolutionary pathway based on genetic integration of non-related species. We address symbiogenesis as a multi-stage process, which involves for-mation of superspecific hereditary systems — functionally integral symbiogenomes (under the facultative partners’ interactions) reorganized into the structurally integral hologenomes (in the obligatory symbioses). The best studied case of symbiogenesis is represented by the evolution of the eukaryotic cell based on transformation of symbiotic bacteria into cellular organelles. This evolution is associated with the deep reduction of microsymbionts’ genomes and with allocation of their genes into the hosts. As a result, microsymbionts lost their Genetic INdividuality (GIN), characterized by an ability to implement DNA- and RNA-based template syntheses required for genome maintenance and expression. Under facultative symbiotic dependence on hosts, the par-tial loss of GIN is due to a “symbiont → host” altruism which in the N2-fixing microbe–plant symbioses results in formation of non-reproducible bacterial forms (e.g., intracellular bacteroids in rhizobia or multiple heterocysts in Nostoc). If micro-symbionts lose their ability of autonomous existence (e.g., in the vertically transmitted intracellular symbionts), they are switched to the “forced altruism” in which the GIN reduction is required for the stable persistence of symbionts in hosts. Therefore, organellogenesis involves the sequential increase of the symbionts’ de-pendency on hosts: conditional → facultative → obligatory → absolute. It is associated with the reorganization of microbes into semi-autonomous cellular components, which may be completely devoid of their own genomes.

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Section: Genetic Individuality In Symbiotic Microbesmentioning

confidence: 99%

Genetic individuality and interspecies altruism: modelling symbiogenesis using different types of symbiotic bacteria

Проворов

2021

BioComm

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“…The NoeB protein is predicted to possess sulfuric ester hydrolase activity and membrane localization according to UniProt database (https://www.uniprot.org/). Its specific function remains unknown, nevertheless, sulfation of the lipo-and glucosamine oligosaccharides is known to be important for host specificity during plantmicrobial interactions (Lerouge et al, 1990;Roche et al, 1991). The loss-of-function mutants in noeB cause weak nodule-forming activity and a drastic decrease in the infection thread formation in Medicago littoralis, while in M. truncatula or Melilotus alba these phenotypes were not revealed (Ardourel et al, 1995).…”

Section: Symbio-geneticsmentioning

confidence: 99%

Supply of acetyl-CoA to N<sub>2</sub>-fixing bacteroids: insights from the mutational and proteomic analyses of <em>Sinorhizobium meliloti</em>

Онищук

Курчак

Chizhevskaya

et al. 2021

BioComm

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Rhizobia represent a diverse group of gram-negative bacteria capable of fixing atmospheric nitrogen in symbiosis with leguminous plants. Mechanisms of symbiotic efficiency are important to study not only to reveal the “fine tuning” of the host–symbiont supra-organismal genetic system emergence, but also to develop agriculture with minimal environmental risks. In this paper we demonstrate that among seven genes whose inactivation by Tn5 insertions results in an increased efficiency of rhizobia (Sinorhizobium meliloti) symbiosis with alfalfa (Eff++ phenotype), six genes are involved in the metabolism of small molecules. One of them (SMc04399) encodes for acetate-CoA transferase catalyzing the formation of acetyl-CoA from acyl-CoA. Since acetyl-CoA is required for operation of the Krebs cycle, providing ATP for symbiotic N2 fixation, we suggest that a significant portion of this coenzyme utilized by bacteroids is provided by the plant cell supporting the energy-consuming nitrogenase reaction. Proteomic data analysis allow us to reveal the lability of enzymatic pathways which are involved in bacteroids in the production and catabolism of acetyl-CoA and which should be modified to obtain the Eff++ phenotype. This phenotype was developed also after inactivation of NoeB protein which is involved in the host-specific nodulation and is characterized by an elevated production in wild type S. meliloti bacteroids, suggesting a multifunctional role of noeB in the symbiosis operation.

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“…However, the activation of only one, though key, stage (the trans port of energy substrates into bacteroids) causes a sym biosystem imbalance associated with the competition for the products of photosynthesis between the bacteroids and the plant cells containing them [37]. In this context, inactivation of the eff genes intensifying the cooperation between symbiotic partners seems to be more promising for the construction of effective rhizo Table 2.…”

Section: Mutualistic Symbiosis and Interspecies Altruismmentioning

confidence: 99%

Evolution of host-beneficial traits in nitrogen-fixing bacteria: Modeling and construction of systems for interspecies altruism

Проворов

Vorobyev

2015

Appl Biochem Microbiol

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The literature and our own data on N 2 fixing bacteria forming symbioses with plants and provid ing convenient models to study the evolution of interspecies (microsymbionts → hosts) altruism are consid ered in the review. It is presented as a deeply reorganized intraspecies altruism implemented in the clonal pop ulation of rhizobia (bacteroids → undifferentiated bacteria) under the control of kin selection induced by plant hosts. The analysis of this model suggests that it is possible to engineer practically valuable rhizobial strains in which high N 2 fixing activity is combined with decreased survival outside of plants.

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Construction of highly-effective symbiotic bacteria: Evolutionary models and genetic approaches

Cited by 12 publications

References 37 publications

Genetic individuality and interspecies altruism: modelling symbiogenesis using different types of symbiotic bacteria

Genetic individuality and interspecies altruism: modelling symbiogenesis using different types of symbiotic bacteria

Supply of acetyl-CoA to N<sub>2</sub>-fixing bacteroids: insights from the mutational and proteomic analyses of <em>Sinorhizobium meliloti</em>

Evolution of host-beneficial traits in nitrogen-fixing bacteria: Modeling and construction of systems for interspecies altruism

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