Construction of Glucose-6-Phosphate Dehydrogenase Overexpression Strain of Schizochytrium sp. H016 to Improve Docosahexaenoic Acid Production

Feng, Yue; Zhu, Yongming; Bao, Zhendong; Wang, Bohan; Liu, Tingting; Yu, Tianyi; Yang, Ying; Yu, Liang

doi:10.3390/md21010017

Cited by 5 publications

(2 citation statements)

References 63 publications

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“…Similarly, overexpression of G6PDH in Schizochytrium sp. H016 promoted strain growth and glucose consumption and thus increased the availability of NADPH as well as docosahexaenoic acid production (Sundara Sekar et al, 2017 ; Feng et al, 2022 ). Therefore, increasing the G6PDH expression level in H. marmoreus could efficiently improve the glucose assimilation rate and promote mycelial growth and biomass production, which might contribute to increasing carbon flux into the PPP of H. marmoreus .…”

Section: Resultsmentioning

confidence: 99%

Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield

Lin¹,

Li²,

Ma³

et al. 2023

Front. Microbiol.

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Hypsizygus marmoreus is one of the main industrially cultivated varieties of edible fungi, with a delicious taste and high nutritional value. However, the long harvest period of 130–150 days greatly limits its large-scale expansion. This study aimed to investigate the effects of central carbon metabolism (CCM) on the mycelial growth performance and fruiting body formation of H. marmoreus. Nine edible fungi with different harvest periods were collected and used to evaluate their intracellular carbon metabolic differences in the CCM, which revealed that the imbalanced distribution of intracellular carbon metabolic levels in the CCM of H. marmoreus might be one of the key factors resulting in a slow mycelial growth rate and a long harvest period. Further analysis by three strategies, including metabolomics, adaptation of different carbon sources, and chemical interference, confirmed that low carbon flux into the pentose phosphate pathway (PPP) limited the supply of raw materials, reduced power, and thus influenced the mycelial growth of H. marmoreus. Furthermore, four transformants with increased expression levels of glucose-6-phosphate dehydrogenase (G6PDH), a key rate-limiting enzyme in the PPP of H. marmoreus, were developed and showed more extracellular soluble protein secretion and higher sugar assimilation rates, as well as improved mycelial growth rates in bottle substrate mixtures. Finally, cultivation experiments indicated that the maturation periods of the fruiting body with ~4–5 days in advance and the maximum fruiting body yield of 574.8 g per bag with an increase of 7.4% were achieved by improving the G6PDH expression level of the PPP in H. marmoreus. This study showed that CCM played an important role in the mycelial growth and development of H. marmoreus, which provided new insights for future advancements in cultivating and breeding edible fungi.

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Section: Resultsmentioning

confidence: 99%

Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield

Lin¹,

Li²,

Ma³

et al. 2023

Front. Microbiol.

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“…Schizochytrium is considered an excellent strain for DHA production, but the DHA production is limited by the availability of NADPH. Feng et al found that overexpression of G6PD in Schizochytrium increased the availability of NADPH, which ultimately led to an increase in lipid accumulation and DHA production [51]. The lipid productivity of photosynthetic microalgae is lower than heterotrophic oil-producing microorganisms, due to slower growth caused by photosynthetic autotrophy.…”

Section: Bypass Pathwaysmentioning

confidence: 99%

Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives

Song,

Wang,

Chen

et al. 2024

Marine Drugs

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Microalgal lipids hold significant potential for the production of biodiesel and dietary supplements. To enhance their cost-effectiveness and commercial competitiveness, it is imperative to improve microalgal lipid productivity. Metabolic engineering that targets the key enzymes of the fatty acid synthesis pathway, along with transcription factor engineering, are effective strategies for improving lipid productivity in microalgae. This review provides a summary of the advancements made in the past 5 years in engineering the fatty acid biosynthetic pathway in eukaryotic microalgae. Furthermore, this review offers insights into transcriptional regulatory mechanisms and transcription factor engineering aimed at enhancing lipid production in eukaryotic microalgae. Finally, the review discusses the challenges and future perspectives associated with utilizing microalgae for the efficient production of lipids.

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Improvement of Lipid and Terpenoid Yield in Thraustochytrids Using Chemical Regulators: A Review

Chen,

Yang,

Tong

et al. 2023

Biotechnol Bioproc E

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Construction of Glucose-6-Phosphate Dehydrogenase Overexpression Strain of Schizochytrium sp. H016 to Improve Docosahexaenoic Acid Production

Cited by 5 publications

References 63 publications

Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield

Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield

Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives

Improvement of Lipid and Terpenoid Yield in Thraustochytrids Using Chemical Regulators: A Review

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