Construction of functional biliary epithelial branched networks with predefined geometry using digital light stereolithography

Mazari-Arrighi, Elsa; Ayollo, Dmitry; Farhat, Wissam; Marret, Auriane; Gontran, Emilie; Dupuis-Williams, Pascale; Larghero, Jérôme; Chatelain, François; Fuchs, Alexandra

doi:10.1016/j.biomaterials.2021.121207

Cited by 13 publications

(24 citation statements)

References 42 publications

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“…This selforganization is highly disorganized, branching happens randomly, and structures rarely form stable lumens or vascular networks which could subsequently be manipulated and integrated into a bioengineered tissue construct. We thus developed a technique using DLP bioprinting 24 to control the microenvironmental cues necessary to promote the self-organization of endothelial progenitor cells (EPCs) into branched tubes with various size and shape (Fig 1A ) using a similar experimental process described in 26 . Briefly, a photopolymerizable mix of hydrogels, cells and photoinitiator was placed between a glass coverslip and a PDMS roof, and 26 and on published studies using endothelial cells 32 , we first explored various formulations incorporating either methacrylated gelatin (G MA ) or methacrylated type I collagen (C MA ) supplemented with fibrinogen (FG), methacrylated hyaluronic acid (HA MA ), and lithium phenyl-2,4,6 trimethyl-benzoyl phosphinate (LAP)a cytocompatible photoinitiator 33 (Fig 1B Fig S1).…”

Section: Resultsmentioning

confidence: 99%

“…We thus developed a technique using DLP bioprinting 24 to control the microenvironmental cues necessary to promote the self-organization of endothelial progenitor cells (EPCs) into branched tubes with various size and shape (Fig 1A ) using a similar experimental process described in 26 . Briefly, a photopolymerizable mix of hydrogels, cells and photoinitiator was placed between a glass coverslip and a PDMS roof, and 26 and on published studies using endothelial cells 32 , we first explored various formulations incorporating either methacrylated gelatin (G MA ) or methacrylated type I collagen (C MA ) supplemented with fibrinogen (FG), methacrylated hyaluronic acid (HA MA ), and lithium phenyl-2,4,6 trimethyl-benzoyl phosphinate (LAP)a cytocompatible photoinitiator 33 (Fig 1B Fig S1). Indeed, G MA and C MA are photopolymerizable hydrogels produced from gelatin and collagen respectively which sustain good cell viability following encapsulation and retain natural cell-binding motifs [34][35][36] .…”

Section: Resultsmentioning

confidence: 99%

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Self-organization of Long-lasting Human Endothelial Capillary Networks guided by DLP Bioprinting

Mazari-Arrighi

Ayollo

Lépine

et al. 2023

Preprint

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Tissue engineering holds great promise for regenerative medicine, drug discovery and as an alternative to animal models. However, as soon as the dimensions of engineered tissue exceed the diffusion limit of oxygen and nutriments, a necrotic core forms leading to irreversible damage. To overcome this constraint, the establishment of a functional perfusion network is essential and is a major challenge to be met. In this work, we explore a promising Digital Light Processing (DLP) bioprinting approach to encapsulate endothelial progenitor cells (EPCs) in 3D photopolymerized hydrogel scaffolds to guide them towards vascular network formation. We observed that EPCs encapsulated in the appropriate photopolymerized hydrogel can proliferate and self-organize within a few days into branched tubular structures with predefined geometry, forming capillary-like vascular tubes or trees of various diameters (in the range of 10 to 100 um). Presenting a monolayer wall of endothelial cells strongly connected by tight junctions around a central lumen, these structures can be microinjected with fluorescent dye and are stable for several weeks in vitro. Interestingly, our technology has proven to be versatile in promoting the formation of vascular structures using a variety of vascular cell lines, including EPCs, human vascular endothelial cells (HUVECs) and human dermal lymphatic endothelial cells (HDLECs). We have also demonstrated that these vascular structures can be recovered and manipulated in an alginate patch without altering their shape or viability. This opens new opportunities for future applications, such as stacking these endothelial vascular structures with other cell sheets or multicellular constructs to yield bioengineered tissue with higher complexity and functionality.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Self-organization of Long-lasting Human Endothelial Capillary Networks guided by DLP Bioprinting

Mazari-Arrighi

Ayollo

Lépine

et al. 2023

Preprint

Self Cite

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“…The printer only needs a movable platform in the z-axis direction, improving the printing efficiency with a sufficiently high spatial resolution. SLA has been used to fabricate a variety of complex cell-laden structures including vascular networks [79,[89][90][91][92][93][94].…”

Section: Stereolithography (Sla) Bioprintingmentioning

confidence: 99%

Bioprinting Technologies and Bioinks for Vascular Model Establishment

Kong

Wang

2023

IJMS

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Clinically, large diameter artery defects (diameter larger than 6 mm) can be substituted by unbiodegradable polymers, such as polytetrafluoroethylene. There are many problems in the construction of small diameter blood vessels (diameter between 1 and 3 mm) and microvessels (diameter less than 1 mm), especially in the establishment of complex vascular models with multi-scale branched networks. Throughout history, the vascularization strategies have been divided into three major groups, including self-generated capillaries from implantation, pre-constructed vascular channels, and three-dimensional (3D) printed cell-laden hydrogels. The first group is based on the spontaneous angiogenesis behaviour of cells in the host tissues, which also lays the foundation of capillary angiogenesis in tissue engineering scaffolds. The second group is to vascularize the polymeric vessels (or scaffolds) with endothelial cells. It is hoped that the pre-constructed vessels can be connected with the vascular networks of host tissues with rapid blood perfusion. With the development of bioprinting technologies, various fabrication methods have been achieved to build hierarchical vascular networks with high-precision 3D control. In this review, the latest advances in 3D bioprinting of vascularized tissues/organs are discussed, including new printing techniques and researches on bioinks for promoting angiogenesis, especially coaxial printing, freeform reversible embedded in suspended hydrogel printing, and acoustic assisted printing technologies, and freeform reversible embedded in suspended hydrogel (flash) technology.

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“…[89] Despite not being yet particularly popular in the field, the 3Dbioprinting of cholangiocytes derived from iPSCs, hepatocytes progenitor cells, or even from embryonic stem cells, is documented in the very recent literature, especially for bile ducts engineering applications. [90][91][92]…”

Section: Cells For Liver Bioinksmentioning

confidence: 99%

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

Guagliano

Volpini

Briatico‐Vangosa

et al. 2022

Macromolecular Bioscience

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The main problems in drug development are connected to enormous costs related to the paltry success rate. The current situation empowered the development of high-throughput and reliable instruments, in addition to the current golden standards, able to predict the failures in the early preclinical phase. Being hepatotoxicity responsible for the failure of 30% of clinical trials, and the 21% of withdrawal of marketed drugs, the development of complex in vitro models (CIVMs) of liver is currently one of the hottest topics in the field. Among the different fabrication techniques, 3D-bioprinting is emerging as a powerful ally for their production, allowing the manufacture of three-dimensional constructs characterized by computer-controlled and customized geometry, and inter-batches reproducibility. Thanks to these, it is possible to rapidly produce tailored cell-laden constructs, to be cultured within static and dynamic systems, thus reaching a further degree of personalization when designing in vitro models. This review highlights and prioritizes the most recent advances related to the development of CIVMs of the hepatic environment to be specifically applied to pharmaceutical research, with a special focus on 3D-bioprinting, since the liver is primarily involved in the metabolism of drugs.

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Construction of functional biliary epithelial branched networks with predefined geometry using digital light stereolithography

Cited by 13 publications

References 42 publications

Self-organization of Long-lasting Human Endothelial Capillary Networks guided by DLP Bioprinting

Self-organization of Long-lasting Human Endothelial Capillary Networks guided by DLP Bioprinting

Bioprinting Technologies and Bioinks for Vascular Model Establishment

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

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