Abstract:Objective: To synthesize the gene of human dominant-negative transforming growth factor beta receptor (DNTβRⅡ) by means of oligo chemic synthesis and PCR amplification, construct pTAR-GET-DNTβRⅡ eukaryotic expression vector and investigate whether its expression in breast cancer COS-7 cells.
Methods:The DNTβRⅡ gene fragment was inserted into pTAR-GET to conduct a eukaryotic plasmid, then sequenced and identified by restrictive endonuclease digestion. The eukaryotic expression vector pTAR-GET-DNTβRⅡ was constru… Show more
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