Construction of electrochemical DNA biosensors for investigation of potential risk chemical and physical agents

Svitková, Veronika; Labuda, Ján

doi:10.1007/s00706-017-2012-y

Cited by 7 publications

(4 citation statements)

References 109 publications

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“…Carbon based electrodes display low background current, large potential range, and simple surface regeneration procedure (Bukkitgar, 2016). Biological matrices usually contain low and high molecular weight substances that interfere with the detection of analytes and diminish the sensor response by unwanted signal interferences (Svitkova and Labuda, 2017). These effects can be eliminated by a modification of the sensor surface with protective membranes (Ambrozy, 2013).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of purine metabolites by membrane modified electrochemical sensors

Blaškovičová

Purdešová

2022

Acta Chimica Slovaca

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Purine metabolites are important for metabolic and cellular processes. Deregulation of purinergic signaling leads to pathological accumulation of purine degradation products in extracellular fluids and indicates various diseases. In clinical diagnosis at early stages of related diseases, accurate detection of Uric acid and Xanthine is of high importance. Electrochemical methods are fast, simple, sensitive, more convenient, and cost-effective compared to other analytical methods used in purine metabolites signaling. Electrochemical sensors are able to detect more compounds simultaneously. Modification of a glassy carbon electrode sensor with external protective membranes was used in this study to avoid unwanted signal interferences from analyte matrices. Polyvinyl alcohol, Chitosan, and Nafion membranes were selected for sensor modification to compare the electro-neutral, positive and negative charged setting of the Xanthine and Uric acid detection. All three membrane modified sensors showed adequate stability in the phosphate buffer solution after 5 min of incubation and are thus suitable for simultaneous detection of purine metabolites. The best results in anodic peak current response values were observed using the Nafion membrane modified glassy carbon electrode sensor. The approach reported here can be useful for the detection of purine metabolites from various matrices at early stages of clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Simultaneous detection of purine metabolites by membrane modified electrochemical sensors

Blaškovičová

Purdešová

2022

Acta Chimica Slovaca

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“…DNA sensors with electrochemical transduction systems show undisputable advantages over the conventional techniques described above due to high sensitivity of the signal, universal approaches to biochemical recognition detection, compatibility with common measurement equipment, well elaborated theory, cheap design, low specific requirements to the quality of labor staff, and simple sample treatment protocols [15].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor Based on Poly(Azure B)-DNA Composite for Doxorubicin Determination

Porfireva

Vorobev

Babkina

et al. 2019

Sensors

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A new voltammetric DNA sensor has been developed for doxorubicin determination on the platform of a glassy carbon electrode (GCE) covered with electropolymerized Azure B film and physically adsorbed native DNA. The redox properties of polymeric Azure B were monitored at various pH and scan rates. DNA application decreased the peak currents related to polymeric and monomeric forms of the dye, whereas incubation in doxorubicin solution partially restored the peaks in accordance with the drug and DNA concentration. The relative shift of the cathodic peak current caused by doxorubicin depended on the nominal DNA concentration and its application mode. In optimal conditions, the DNA sensor makes it possible to determine between 0.1 μM to 0.1 nM doxorubicin (limit of detection 7 × 10−11 M). The DNA sensor was tested on commercial doxorubicin formulations and on artificial samples the mimicked electrolyte content of human serum.

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“…A DNA based electrochemical biosensor is typically composed of an electrode used as the transducer and covered by a DNA layer as the biological recognition element which chemically modifies the electrode surface [21]. Changes in physical and chemical DNA structures are reflected in its redox behavior and the DNA based biosensors are able to recognize and determine an extent of specific interactions of DNA with substances of interest [22][23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

DNA Interaction with 17α‐Ethinylestradiol Studied Using Electrochemical Biosensors and Biosensing in Solution

et al. 2019

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The synthetic estrogen 17α‐ethinylestradiol (EE2) is an active component of oral contraceptives. It is considered as an endocrine disrupting compound that, once incorporated into an organism, affects the hormonal balance of animals and humans. In this study we characterized the DNA‐EE2 interaction using an electrochemical biosensor and biosensing in solution phase with the double stranded DNA from salmon sperm and deoxyguanosine monophosphate (dGMP). Differential pulse voltammetry method has been applied based on voltammetric anodic responses of the deoxyguanine (dGuo) and deoxyadenine (dAdo) as well as EE2 in the medium of phosphate buffer solution pH 7.0. Binding of EE2 to the nucleobases leads to a decrease of their anodic signals. Association constant for DNA‐EE2 interaction has been estimated to be about 1.1 ⋅ 103 L mol−1 and 1.4 ⋅ 103 L mol−1 for dGuo and dAdo responses, respectively. The association is reversible as indicated by decrease of the EE2 response in pure buffer solution due to leaching of EE2 from the surface attached DNA. The DNA‐EE2 association has been confirmed also by UV‐vis spectrometric experiments.

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Construction of electrochemical DNA biosensors for investigation of potential risk chemical and physical agents

Cited by 7 publications

References 109 publications

Simultaneous detection of purine metabolites by membrane modified electrochemical sensors

Simultaneous detection of purine metabolites by membrane modified electrochemical sensors

Electrochemical Sensor Based on Poly(Azure B)-DNA Composite for Doxorubicin Determination

DNA Interaction with 17α‐Ethinylestradiol Studied Using Electrochemical Biosensors and Biosensing in Solution

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