Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection

Abstract

To distinguish the cytoplasm of Danio rerio from that of Gobiocypris rarus, we cloned G. rarus COXI and constructed cytoplasmic molecular markers at the high identity domains of COXI by mutated primer PCR (MP-PCR for short). Then Sybr Green I was used to detect the single amplicon. As a result, we succeeded in getting the cytoplasmic molecular markers, G.M COXI and Z.M COXI, by MP-PCR strategy. They were used to detect the sperm-derived mtDNA in the sexual hybrid embryos (D. rerio $ · G. rarus #) before the sphere stage. In the present study, all results demonstrate that MP-PCR approach and Sybr Green I detection are feasible to construct the molecular markers to identify genes that shared high identity.

Morphological Variation Among Wild Populations of Chinese Rare Minnow (Gobiocypris rarus): Deciphering the Role of Evolutionary Processes

“…All known habitats of rare minnow are scattered, separated by dozens to hundreds of miles, indicating a discontinuous distribution for the species. Meanwhile, this fish has been used extensively as an aquatic laboratory animal in toxicology, fish pathology, developmental biology, and genetics (Wang, 1992(Wang, , 1996(Wang, , 1999Wang et al, 1994;Wang and Cao, 1997;Jia et al, 2002;Zhong et al, 2005;Pei et al, 2008;Su et al, 2008). Very little is known about the adaptation of this species to environmental factors, which limits the setting of appropriate conservation plans (but see Shao et al, 2007;He et al, 2012).…”