Construction of comb-like poly(N-isopropylacrylamide) layers on microporous polypropylene membrane by surface-initiated atom transfer radical polymerization

Wan, Ling‐Shu; Yang, Yong; Tian, Jing; Hu, Meng‐Xin; Xu, Zhi‐Kang

doi:10.1016/j.memsci.2008.11.032

Cited by 45 publications

(8 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two strong peaks at 1646 and 1538 cm À1 are the characteristic amide I band (C=O stretching) and amide II band (N-H stretching) of PNIPAAm, respectively (Figure 1(b)). The similar data reported by other researchers [28,29] confirmed that PNIPAAm was successfully grafted onto PHEMA beads by using ATRP reaction. The presence of PNIPAAm grafting was also confirmed by ESCA.…”

Section: Characterization Of Phema-g-pnipaam Microcarrierssupporting

confidence: 87%

Thermosensitive PHEMA microcarriers: ATRP synthesis, characterization, and usabilities in cell cultures

Gümüşderelı́oğlu

Çakmak

Timuçin

et al. 2013

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

In this study, we developed a novel microcarrier to enhance the production of anchorage-dependent mammalian cells in large scale by preserving them from the effects of shear forces and to enhance their removal from the surface without using proteolytic enzymes and chelating agents. This 'thermosensitive microcarrier' was synthesized by the grafting thermoresponsive molecule, N-isopropylacrylamide (NIPAAm), to the crosslinked poly(2-hydroxyethyl methacrylate) (PHEMA) beads by surface-initiated atom transfer radical polymerization. NIPAAm was polymerized on bromine-activated beads' surfaces to prepare PHEMA-g-PNIPAAm microcarriers. Then, they were chemically characterized by attenuated total reflectance Fourier transform infrared and electron spectroscopy for chemical analysis. Surface morphologies were further investigated by scanning electron microscope and atomic force microscopy techniques. The results of characterization studies confirmed that PNIPAAm was successfully grafted onto PHEMA beads by the means of atom transfer radical polymerization reaction. The cellular activities of PHEMA-g-PNIPAAm microcarriers were evaluated at static and dynamic culture conditions by using two types of cell lines with different morphology, i.e. L929 mouse fibroblasts and HS2 epithelial human keratinocytes. The microcarriers exhibited better cell adhesion and proliferation characteristics for both cell lines. Although their thermally induced cell detachment efficiencies are lower than that of trypsinization, thermally harvested cells preserved their surface morphologies and proliferation characteristics.

show abstract

Section: Characterization Of Phema-g-pnipaam Microcarrierssupporting

confidence: 87%

Thermosensitive PHEMA microcarriers: ATRP synthesis, characterization, and usabilities in cell cultures

Gümüşderelı́oğlu

Çakmak

Timuçin

et al. 2013

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

show abstract

“…Recently, PNIPAAm was also grafted onto other porous substrates, including nylon‐6, poly(vinylidene fluoride) (PVDF),83 polycarbonate (PC),84 polypropylene,85 poly(ethyleneterephthalate) (PET),86 polysulfone,87 and SiO 2 nanoparticles deposited on Shirasu porous glass,88 and the temperature‐controlled permeability of membranes was evaluated by different kinds of solution. Among them, a work reported by Schepelina and Zharov deserves special attention 89.…”

Section: Biologically Related Applicationsmentioning

confidence: 99%

Biomimetic Smart Interface Materials for Biological Applications

2011

View full text Add to dashboard Cite

Controlling the surface chemical and physical properties of materials and modulating the interfacial behaviors of biological entities, e.g., cells and biomolecules, are central tasks in the study of biomaterials. In this context, smart polymer interface materials have recently attracted much interest in biorelated applications and have broad prospects due to the excellent controllability of their surface properties by external stimuli. Among such materials, poly(N-isopropylacrylamide) and its copolymer films are especially attractive due to their reversible hydrogen-bonding-mediated reversible phase transition, which mimics natural biological processes. This platform is promising for tuning surface properties or to introduce novel biofunctionalities via copolymerization with various functional units and/or combination with other materials. Important progress in this field in recent years is highlighted.

show abstract

“…The anchoring of the initiator onto the surface is a precondition in SI-ATRP of various monomers [28]. Organic halides with a potentially active carbon-halogen bond have been widely used as initiators for ATRP of various monomers.…”

Section: Cmpsf Membrane and Cmpsf-g-p4vp Membranementioning

confidence: 99%

Synthesis and antibacterial activity of copper-immobilized membrane comprising grafted poly(4-vinylpyridine) chains

Qiu

Zhang

et al. 2011

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

Construction of comb-like poly(N-isopropylacrylamide) layers on microporous polypropylene membrane by surface-initiated atom transfer radical polymerization

Cited by 45 publications

References 43 publications

Thermosensitive PHEMA microcarriers: ATRP synthesis, characterization, and usabilities in cell cultures

Thermosensitive PHEMA microcarriers: ATRP synthesis, characterization, and usabilities in cell cultures

Biomimetic Smart Interface Materials for Biological Applications

Synthesis and antibacterial activity of copper-immobilized membrane comprising grafted poly(4-vinylpyridine) chains

Contact Info

Product

Resources

About