Construction of Co@C Hybrid Nanostructure: Electrochemical Biosensor for Detection of Penicillin Sodium in Milk

Xiu, Yi; Luo, Ruiping; Han, Baoqing; Liu, Lu; Wang, Hongsu

doi:10.1007/s12161-019-01677-3

Cited by 17 publications

(13 citation statements)

References 34 publications

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“…An enzyme‐immobilized material was obtained and stored at 4°C. The amount of penicillinase, relative enzyme activity, and immobilized enzyme activity were determined according to the method in the literature (Xiu et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

“…Afterwards, 2 mL of Pen X at specific concentrations were added at certain temperatures and different speeds for a period; after centrifugation and repeated washing, the upper washing solution was collected for later determination of Pen X loading. An enzyme-immobilized material was obtained and stored at 4 • C. The amount of penicillinase, relative enzyme activity, and immobilized enzyme activity were determined according to the method in the literature (Xiu et al, 2019).…”

Section: Immobilization Of Penicillinase and Determination Of Enzyme Activitymentioning

confidence: 99%

“…MIPs AMP 1-500 nM 1.89 nM (Jamieson et al, 2019) Pen X/Co @ C-PMB-GCE Pen G 0.27-2.7 and 2.7-27 nM 1.7 nM (Xiu et al, 2019) Pen X -NiNPs -SPCE Pen G 0.01-0.5 M 3.1×10 5 nM (Salihu et al, 2019) anti…”

Section: Working Electrode Antibiotic Detection Range Detection Limit Referencesmentioning

confidence: 99%

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Penicillin biosensor based on rhombus‐shaped porous carbon/hematoxylin/penicillinase

Wang

Xiu

et al. 2021

Journal of Food Science

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In this experiment, we designed an electrochemical sensor using penicillinase (Pen X)-rhombus porous carbon (RPC) as the detection element and hematoxylin as the indicator to detect low concentrations of penicillin sodium (Pen G). A differential pulse voltammetry (DPV) method was used to detect Pen G in the concentration range of 10 -8 -10 -5 mg⋅mL -1 under optimal experimental conditions. The results showed that the peak current value and the logarithm of Pen G concentration showed a good linear relationship (R 2 = 0.9915), and the LOD was 2.68 × 10 -7 mg⋅mL -1 (S/N = 3). The actual milk samples were detected by the addition method and compared with the high-performance liquid phase method; no significant difference was found in the detection results. The working electrode prepared by cross-linking method not only extends the service life of the sensor, but also improves the sensitivity and reproducibility of the sensor. It can also be used to detect the Pen G residue in the actual milk samples repeatedly. Practical Application: In this study, an electrochemical sensor for the rapid detection of penicillin sodium in milk was prepared, which has good sensitivity and fast detection speed.

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Section: Methodsmentioning

confidence: 99%

Section: Immobilization Of Penicillinase and Determination Of Enzyme Activitymentioning

confidence: 99%

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Penicillin biosensor based on rhombus‐shaped porous carbon/hematoxylin/penicillinase

Wang

Xiu

et al. 2021

Journal of Food Science

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“…The immobilization of penicillinase was determined by the following formula: (Xiu, Luo, Han, Liu, & Wang, 2019)

Q = (C_{0} V_{0} - C_{1} V_{1}) / W

where Q is the penicillinase fixed capacity (mg/g); C 0 is the penicillinase concentration in the initial solution (mg/L); C 1 is the penicillinase concentration in the supernatant after immobilization (mg/L); V 0 is the initial solution volume (mL); V 1 is the total volume of supernatant after β‐lactamase immobilization (mL); and W is the mass of MHM material (g).…”

Section: Methodsmentioning

confidence: 99%

Magnetic mesoporous carbon material based electrochemical sensor for rapid detection of penicillin sodium in milk

Wang

Xiu

Han

et al. 2020

Journal of Food Science

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In recent years, to increase growth rate and prevent infectious diseases, an excessive use of antibiotics in livestock breeding processes has resulted in the presence of antibiotic residues in animal foods. In this experiment, a new kind of electrochemical sensor is prepared based on magnetic mesoporous hollow carbon microspheres (MHM) as a penicillinase (Pen X) adsorption carrier to rapidly detect penicillin sodium (Pen G), named Pen X/MHM/MGCE. The MHM‐adsorbed penicillinase can be separated from the solution by magnetic attraction and fixed on a magnetic glassy carbon electrode by physical adsorption, which is easy to operate and avoids the interference of a crosslinking agent at the active site of the enzyme. A differential pulse voltammetry (DPV) method is used to immerse the working electrode in test samples containing different concentrations of penicillin sodium solution with 0.5 mg/mL oxidized hematoxylin. According to the quantitative relationship between the current value and the concentration of penicillin sodium, the concentration of penicillin sodium in the tested samples can be determined. The detection range of the sensor is 10−8 to 10−2 mg/mL, the linear relationship is good (R2 = 0.9983), and the detection limit (LOD) is 2.655 × 10−7 mg/mL (S/N = 3). The detection of penicillin sodium in milk using a standard addition method shows good recovery. Furthermore, the proposed method has the advantages of a wide detection range, good enzyme immobilization capacity, good precision and stability, convenient cleaning, and recycling of solid materials. Thus, it can successfully achieve rapid detection in milk samples. Practical Application The sensor provides a low detection limit and high recovery rate of electrode material; therefore, the sensor can realize the rapid and quantitative detection of penicillin sodium in milk.

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“…[11][12][13] Sensors are analytical devices that have a combination of simple and convenient converter receptors containing biological recognition elements. [14] In the last years, electrochemical [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] and optical sensors based on fluorometric and colorimetric measurements [30][31][32][33] have been used for the estimation of penicillin antibiotics due to their high reactivity and selectivity. Surface plasmon resonance (SPR) is an optical technique based on the intensity of reflected light in a prism covered with a thin metal film.…”

Section: Introductionmentioning

confidence: 99%

Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk

et al. 2021

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In this study, selective and sensitive Penicillin G (PEN−G) antibiotic detection from both aqueous solution and milk samples using molecular imprinting technique was performed by surface plasmon resonance sensor. For this purpose, PEN−G imprinted poly(2‐Hydroxyethyl methacrylate‐N‐methacroyl‐(L)‐cysteine methyl ester‐gold nanoparticles‐N‐methacryloyl‐L‐phenylalanine methyl ester (MIP‐AuNPs) nanosensor was prepared. Control experiments were carried out via the nanosensor without the addition of AuNPs (MIP) to examine the effect of gold nanoparticles (AuNPs) incorporated to increase the surface plasmon resonance signal response. Moreover, to evaluate the imprinting efficiency, non‐imprinted (NIP‐AuNPs) nanosensor was designed using the same polymerization recipe except the addition of the PEN−G molecule. Characterization studies of MIP, MIP‐AuNPs and NIP‐AuNPs nanosensors were performed with FTIR‐ATR spectrophotometer. Also, MIP‐AuNPs and NIP‐AuNPs nanosensors were characterized by atomic force microscopy, ellipsometer and contact angle measurements. Imprinting efficiency (I.F: 7.83) for the MIP‐AuNPs nanosensor was determined by comparing it with the NIP‐AuNPs nanosensor. The MIP‐AuNPs nanosensor was 9.87 times more selective for the target PEN−G molecule than amoxicillin, and 16.78‐times than ampicillin. In addition, the amount of PEN−G in milk selected as a real sample was measured by spiking 5 ppb PEN−G into the milk.

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Construction of Co@C Hybrid Nanostructure: Electrochemical Biosensor for Detection of Penicillin Sodium in Milk

Cited by 17 publications

References 34 publications

Penicillin biosensor based on rhombus‐shaped porous carbon/hematoxylin/penicillinase

Penicillin biosensor based on rhombus‐shaped porous carbon/hematoxylin/penicillinase

Magnetic mesoporous carbon material based electrochemical sensor for rapid detection of penicillin sodium in milk

Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk

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