Construction of chemiluminescence aptasensor platform using magnetic microsphere for ochratoxin A detection based on G bases derivative reaction and Au NPs catalyzing luminol system

Yan, Xiaojun; Xue, Xinxin; Luo, Jing; Jian, Yuting; Li, Tong; Zheng, Xiangjuan

doi:10.1016/j.snb.2020.128375

Cited by 27 publications

(6 citation statements)

References 37 publications

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“…Compared with conventional OTA detection methods, such as instrumental methods including high-performance liquid chromatography (HPLC) and liquid chromatography–mass spectrometry (LC–MS), immunoassay methods such as enzyme-linked immunosorbent assays and gold immunochromatographic assays, and other newly developed detection techniques using nucleic acid aptamers, such as electrochemical, ,− chemiluminescent, colorimetric, , fluorescent, ,, and surface-enhanced Raman scattering, ,, the present sensor system has a lower detection limit and a linear detection range of more than 5 orders of magnitude, showing better analytical performance for OTA detection. In addition, compared with the dissociation constant K d of 200 nM for the original OTA aptamer, an apparent dissociation constant K d of 0.169 nM was obtained when fitting Figure to a binding curve (shown in Figure S6), which is much smaller because of the DNAzyme-based amplification effect.…”

Section: Resultsmentioning

confidence: 99%

DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter

Zhang

Luan

Xiong

et al. 2021

ACS Appl. Mater. Interfaces

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Aptamer-based sensors have emerged as a major platform for detecting small-molecular targets, because aptamers can be selected to bind these small molecules with higher affinity and selectivity than other receptors such as antibodies. However, portable, accurate, sensitive, and affordable detection of these targets remains a challenge. In this work, we developed an aptasensing platform incorporating magnetic beads and a DNAzyme for signal amplification, resulting in high sensitivity. The biosensing platform was constructed by conjugating a biotin-labeled aptamer probe of small-molecular targets such as toxins and a biotinlabeled substrate strand on magnetic beads, and the DNAzyme strand hybridized with the aptamer probe to block the substrate cleavage activity. The specific binding of the small-molecular target by the aptamer probe can replace the DNAzyme strand and then induce the hybridization between the DNAzyme strand and substrate strand, and the iterative signal amplification reaction of hydrolysis and cleavage of the substrate chain occurs in the presence of a metal ion cofactor. Using invertase to label the substrate strand, the detection of small molecules of the toxin is successfully transformed into the measurement of glucose, and the sensitive analysis of small molecules such as toxins can be realized by using the household portable glucose meter as a readout. This platform is shown to detect ochratoxin, a common toxin in food, with a linear detection range of 5 orders of magnitude, a low detection limit of 0.88 pg/mL, and good selectivity. The platform is easy to operate and can be used as a potential choice for quantitative analysis of small molecules, at home or under point-of-care settings. Moreover, by changing and designing the aptamer probe and the arm of DNAzyme strand, it can be used for the analysis of other analytes.

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Section: Resultsmentioning

confidence: 99%

DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter

Zhang

Luan

Xiong

et al. 2021

ACS Appl. Mater. Interfaces

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“…Magnetic nanoparticles (MNPs) have also been used as a nanoassembling template for bioassay targeting, either alone or in combination with other nanostructures, where they can be easily isolated from the solution via an external magnetic field [ 39 , 102 , 103 , 104 , 105 ]. Miao et al reported a sensitive pH-based colorimetric strategy based on glucose oxidase (GOD) enrichment and catalysis for the amplified detection of cancer biomarkers with isothermal DNA signal amplification and multibranched rolling circle amplification (mb-RCA) [ 103 ].…”

Section: Colorimetric Aptasensormentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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“…As the exciting alternatives, nanozymes can largely enhance the sensitivity of CL aptasensors (Huang, Ren, et al, 2019). By using MBs as the separated carrier, Yan et al (2020) fabricated two kinds of CL aptasensors on the surface of MBs for OTA detection. The label-free aptasensor was proposed based on the transient derivation reaction between guanine (G) bases of aptamers and phenylglyoxal.…”

Section: Aptasensorsmentioning

confidence: 99%

Aptasensors for mycotoxins in foods: Recent advances and future trends

Hou

Jia

Sheng

et al. 2021

Comp Rev Food Sci Food Safe

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Mycotoxin contamination in foods has posed serious threat to public health and raised worldwide concern. The development of simple, rapid, facile, and cost‐effective methods for mycotoxin detection is of urgent need. Aptamer‐based sensors, abbreviated as aptasensors, with excellent recognition capacity to a wide variety of mycotoxins have attracted ever‐increasing interest of researchers because of their simple fabrication, rapid response, high sensitivity, low cost, and easy adaptability for in situ measurement. The past few decades have witnessed the rapid advances of aptasensors for mycotoxin detection in foods. Therefore, this review first summarizes the reported aptamer sequences specific for mycotoxins. Then, the recent 5‐year advancements in various newly developed aptasensors, which, according to the signal output mode, are divided into electrochemical, optical and photoelectrochemical categories, for mycotoxin detection are comprehensively discussed. A special attention is taken on their strengths and limitations in real‐world application. Finally, the current challenges and future perspectives for developing novel highly reliable aptasensors for mycotoxin detection are highlighted, which is expected to provide powerful references for their thorough research and extended applications. Owing to their unique advantages, aptasensors display a fascinating prospect in food field for safety inspection and risk assessment.

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Construction of chemiluminescence aptasensor platform using magnetic microsphere for ochratoxin A detection based on G bases derivative reaction and Au NPs catalyzing luminol system

Cited by 27 publications

References 37 publications

DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter

DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter

Recent Advances in Aptamer Sensors

Aptasensors for mycotoxins in foods: Recent advances and future trends

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