Construction of ceRNA network and key gene screening in cervical squamous intraepithelial lesions

Abstract

Background: This study aimed to construct an endogenous competition network for cervical squamous intraepithelial lesions using differential gene screening. Methods: GSE149763 was used to screen differentially expressed long non-coding RNAs (lncRNAs) and mRNAs to predict correlated microRNAs (miRNAs). The correlated miRNAs and GSE105409 were used to screen differentially expressed miRNAs for differential co-expression analysis, and the co-expressed differentially expressed miRNAs were used to predict correlated mRNAs. Differentially expressed mRNAs, miRNAs, and lncRNAs were visualized, and differential gene screening, enrichment, and pathway analysis were performed. Results: The ceRNA network of cervical squamous intraepithelial was successfully established and a potential differentially expressed network was identified. The key genes were VEGFA and FOS, and the key pathway was the MAPK signaling pathway. Conclusions: The differential expression and potential effects of the lncRNA BACH1-IT1/miR-140-5p/VEGFA axis, key genes, VEGFA and FOS, and MAPK signaling in CIN were clarified, and the occurrence and potential effects of CIN were further clarified. The underlying molecular mechanism provides a certain degree of reference for subsequent treatments and experimental research.

mRNA-lncRNA gene expression signature in HPV-associated neoplasia and cervical cancer

“…The aim of this work was to perform bioinformatics analysis of RNA sequencing data from epitheliocytes of women with cervical epithelial neoplasia and cervical cancer based on open data from three different studies (Royse et al, 2014;Hu et al, 2015;Qi et al, 2022).…”