Construction of catalase@hollow silica nanosphere: Catalase with immobilized but not rigid state for improving catalytic performances

Du, Yingjie; Zhao, Lixue; Geng, Zixin; Huo, Zibei; Li, Huihui; Shen, Xuejian; Peng, Xiaogang; Yan, Renyi; Cui, Jiandong; Jia, Shiru

doi:10.1016/j.ijbiomac.2024.130381

Cited by 8 publications

(2 citation statements)

References 35 publications

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“…Calculation ΔA = A1 – A2. CAT activity was determined by measuring the remaining amount of H 2 O 2 ( Du et al, 2024 ). Specifically, 10.0 μL of CAT was added to a 0.033 M H 2 O 2 solution and allowed to react at 25°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Endophyte-mediated enhancement of salt resistance in Arachis hypogaea L. by regulation of osmotic stress and plant defense-related genes

Liang,

Tan,

Chen

et al. 2024

Front. Microbiol.

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IntroductionSoil salinization poses a significant environmental challenge affecting plant growth and agricultural sustainability. This study explores the potential of salt-tolerant endophytes to mitigate the adverse effects of soil salinization, emphasizing their impact on the development and resistance of Arachis hypogaea L. (peanuts).MethodsThe diversity of culturable plant endophytic bacteria associated with Miscanthus lutarioriparius was investigated. The study focused on the effects of Bacillus tequilensis, Staphylococcus epidermidis, and Bacillus siamensis on the development and germination of A. hypogaea seeds in pots subjected to high NaCl concentrations (200 mM L−1).ResultsUnder elevated NaCl concentrations, the inoculation of endophytes significantly (p < 0.05) enhanced seedling germination and increased the activities of enzymes such as Superoxide dismutase, catalase, and polyphenol oxidase, while reducing malondialdehyde and peroxidase levels. Additionally, endophyte inoculation resulted in increased root surface area, plant height, biomass contents, and leaf surface area of peanuts under NaCl stress. Transcriptome data revealed an augmented defense and resistance response induced by the applied endophyte (B. tequilensis, S. epidermidis, and B. siamensis) strain, including upregulation of abiotic stress related mechanisms such as fat metabolism, hormones, and glycosyl inositol phosphorylceramide (Na+ receptor). Na+ receptor under salt stress gate Ca2+ influx channels in plants. Notably, the synthesis of secondary metabolites, especially genes related to terpene and phenylpropanoid pathways, was highly regulated.ConclusionThe inoculated endophytes played a possible role in enhancing salt tolerance in peanuts. Future investigations should explore protein–protein interactions between plants and endophytes to unravel the mechanisms underlying endophyte-mediated salt resistance in plants.

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Section: Methodsmentioning

confidence: 99%

Endophyte-mediated enhancement of salt resistance in Arachis hypogaea L. by regulation of osmotic stress and plant defense-related genes

Liang,

Tan,

Chen

et al. 2024

Front. Microbiol.

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“…Metal–organic frameworks, also known as porous coordination polymers, represent an emerging class of three-dimensional ordered porous materials formed by the linkage between organic ligands and metal nodes via ordination bonds . Their natural advantages, such as ultrahigh porosity, large surface area, high structural tunability, and functional designability, lead to a great potential for various applications including gas storage and separations, catalysis, biomedicine, proton conduction, etc. , In particular, there are growing appeals for exploring MOFs as potent supports for enzyme immobilization, endowed by the high surface area, the facile de novo design, and postsynthetic modification. , To date, different modification strategies have been proposed to regulate the structure and properties of MOFs to exhibit better performance as carrier materials, while various immobilization strategies such as encapsulation and coprecipitation have been proposed to achieve enzyme immobilization within MOFs. − The hydrophilicity and porosity of MOFs have been demonstrated to significantly affect the activity, selectivity, and stability of the immobilized enzymes. , In addition to being carrier materials, MOFs can also be used as the template for the construction of enzyme immobilization . However, the difficulty in constructing ordered and interconnected macroporous structures severely limits the application of MOFs in the immobilization of macromolecular enzymes as well as in bulky-molecule reaction systems .…”

Section: Introductionmentioning

confidence: 99%

Single-Crystalline Ordered Nanoporous Metal–Organic Frameworks for Lipase Immobilization and Structured Lipid Production

Liu,

Li,

Dai

et al. 2024

ACS Appl. Nano Mater.

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Single-crystalline ordered macro-microporous CuBTC shows excellent mass transfer performance and has high fatty acid stability, making it highly promising as an enzyme immobilization carrier. However, its practical application is severely limited by its poor water stability. To address this challenge, we introduced a ligand, 1,2,3-benzotriazole (BTA), known for its hydrophobicity and strong coordination with copper. A series of CuBTCderived dual-ligand SOM-MOFs (SOM-MIXs) was successfully synthesized with enhanced hydrophobicity and improved water stability. The CuBTCderived SOM-MIXs demonstrated superior performance for lipase immobilization, resulting in a maximum increase of 66.9% in specific activity. To address the limitation that excessive addition of 1,2,3-benzotriazole does not further enhance the hydrophobicity of the carrier and may lead to structural damage, a sol−gel method was employed to coat the SOM-MIX with highly hydrophobic PDMS, leading to a further increase of 139.1% in the specific activity. The resulting immobilized lipase exhibited excellent catalytic performance and remarkable reusability, with 90.09% activity retention after five cycles in the synthesis process of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) by acidolysis. This work highlights the potential of SOM-MIX@PDMS and provides valuable insights into the rational design and postmodification of metal−organic frameworks (MOFs) for enzyme immobilization in diverse applications.

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Lipase grafted on magnetic and CO2 dual-responsive Janus nanoparticles for interfacial catalyzed transesterification

Zhang,

Li,

Liu

et al. 2024

Applied Catalysis A: General

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Construction of catalase@hollow silica nanosphere: Catalase with immobilized but not rigid state for improving catalytic performances

Cited by 8 publications

References 35 publications

Endophyte-mediated enhancement of salt resistance in Arachis hypogaea L. by regulation of osmotic stress and plant defense-related genes

Endophyte-mediated enhancement of salt resistance in Arachis hypogaea L. by regulation of osmotic stress and plant defense-related genes

Single-Crystalline Ordered Nanoporous Metal–Organic Frameworks for Lipase Immobilization and Structured Lipid Production

Lipase grafted on magnetic and CO2 dual-responsive Janus nanoparticles for interfacial catalyzed transesterification

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