Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16a

Ye, Rick W.; Kelly, Kristen L.

doi:10.1007/978-1-61779-879-5_10

Cited by 17 publications

(8 citation statements)

References 14 publications

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“…When the isolate was grown on NMS agar plates, it formed light yellow colonies initially, which turned yellow, orange, and brown over time (Table ). Pigmentation is a common phenomenon for type I methanotroph, Methylomonas species . The microorganism was non‐motile, gram negative, and short‐rod shaped with a size of approximately 1 × 1.5 µm (Fig.…”

Section: Resultsmentioning

confidence: 99%

Highly efficient bioconversion of methane to methanol using a novel type I Methylomonas sp. DH‐1 newly isolated from brewery waste sludge

Hur

Lee

2016

J of Chemical Tech & Biotech

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Background Methane is the major component of natural and shale gas. Methane can be converted into methanol via a bioprocess using methanotrophs, and methanol is a valuable chemical feedstock for the production of value‐added chemicals. This work demonstrates highly effective bioconversion of methane to methanol using a newly isolated novel methanotroph, Methylomonas sp. DH‐1. Results A novel methanotroph strain was isolated from activated sludge from a brewery plant and characterized using phylogenetic analysis, electron microscopy and chemotaxonomic analysis. This aerobic, Gram‐negative, non‐motile rod‐shaped type I methanotroph was designated as Methylomonas sp. DH‐1. The growth condition of Methylomonas sp. DH‐1 and batch methane‐to‐methanol bioconversion conditions such as methane concentration, pH, biocatalyst loading, concentration of formate and MDH inhibitor were analyzed and optimized. Methanol was produced from methane with a 1.340 g L−1 titer, a 0.332 g L−1 h−1 volumetric conversion rate and a 0.0752 g g−1 cell h−1 specific methanol conversion rate. Conclusion It was demonstrated that isolation and application of a new methanotroph strain is a practical way of improving bioconversion efficiency in the conversion of methane to methanol. Moreover, one promising feature of Methylomonas sp. DH‐1 for methanol production was its extremely high tolerance to methanol up to 7%(v/v), which is advantageous for high‐titer methanol production. © 2016 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Highly efficient bioconversion of methane to methanol using a novel type I Methylomonas sp. DH‐1 newly isolated from brewery waste sludge

Hur

Lee

2016

J of Chemical Tech & Biotech

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“…Carotenoid compounds have been intensively studied due to their excellent antioxidant effects and their applications in the health and medicine, food additive, cosmetics and feed industries. Methylomonas were reported to synthesize value-added C 30 carotenoids (Tao et al 2005), and 16a was metabolically engineered to synthesize astaxanthin from methane Ye et al 2007;Ye and Kelly 2012). In our study, Methylomonas sp.…”

Section: Carotenoid Synthesis By Zr1mentioning

confidence: 89%

“…Recently, DuPont reported the engineering of Methylomonas sp. 16a to produce the carotenoid astaxanthin from methane Ye et al 2007;Ye and Kelly 2012;Donaldson et al 2015).…”

mentioning

confidence: 99%

Synthesizing value-added products from methane by a new Methylomonas

Guo

et al. 2017

J Appl Microbiol

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“…As an example of metabolic engineering of methanotrophs, total carotenoid synthesis has been increased up to 20‐fold by integrating the genes involved in the synthesis of the C40 carotenoids canthaxanthin and astaxanthin within the chromosome of Methylomonas sp. strain 16a permitting optimal expression using random transposon mutagenesis . Tn 5 transposon‐based promoterless transposable elements containing carotenoid gene clusters were used.…”

Section: Metabolic Engineering Of Methanotrophs For Production Of Chementioning

confidence: 99%

Metabolic engineering of methanotrophs and its application to production of chemicals and biofuels from methane

Lee

Hur

Nguyen

et al. 2016

Biofuels Bioprod Bioref

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Methane‐assimilating bacteria, methanotrophs, can play an important role in producing various value‐added chemicals and biofuels from methane, which is considered a next‐generation carbon feedstock. The capability to engineer the metabolic pathway of methanotrophs is a key success factor for enhancing methane‐to‐product conversion efficiency. Recently, OMICS studies on several model methanotrophs have been conducted and provided strategies to engineer methanotrophs. Here, we present a review on the current progresses and future prospects of metabolic engineering of methanotrophs and its application to chemical and biofuel production from methane. © 2016 Society of Chemical Industry and John Wiley & Sons, Ltd

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Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16a

Cited by 17 publications

References 14 publications

Highly efficient bioconversion of methane to methanol using a novel type I Methylomonas sp. DH‐1 newly isolated from brewery waste sludge

Highly efficient bioconversion of methane to methanol using a novel type I Methylomonas sp. DH‐1 newly isolated from brewery waste sludge

Synthesizing value-added products from methane by a new Methylomonas

Metabolic engineering of methanotrophs and its application to production of chemicals and biofuels from methane

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