Construction of bioartificial renal tubule assist device In Vitro and its function of transporting sodium and glucose

Dong, Xinggang; Chen, Jianghua; He, Qing; Yang, Yi; Zhang, Wei

doi:10.1007/s11596-009-0425-5

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…Concurrently, most of the Cl − is reabsorbed paracellularly due to the generated electrochemical gradient, although Cl − channels and Cl − ‐coupled transporters also contribute to Cl‐ reabsorption (Planelles, ). NRK‐52E cell line is derived from PT and express Na + /glucose cotransporter (Dong, Chen, He, Yang, & Zhang, ). The epithelium of PT has low transepithelial resistance and is considered a “leaky” epithelium, in which the paracellular resistance is much lower than the transcellular resistance.…”

Section: Resultsmentioning

confidence: 99%

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A perfusion chamber for monitoring transepithelial NaCl transport in an in vitro model of the renal tubule

Yeste

Martínez-Gimeno

Illa

et al. 2018

Biotech & Bioengineering

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Transepithelial electrical measurements in the renal tubule have provided a better understanding of how kidney regulates electrolyte and water homeostasis through the reabsorption of molecules and ions (e.g., H2O and NaCl). While experiments and measurement techniques using native tissue are difficult to prepare and to reproduce, cell cultures conducted largely with the Ussing chamber lack the effect of fluid shear stress which is a key physiological stimulus in the renal tubule. To overcome these limitations, we present a modular perfusion chamber for long‐term culture of renal epithelial cells under flow that allows the continuous and simultaneous monitoring of both transepithelial electrical parameters and transepithelial NaCl transport. The latter is obtained from electrical conductivity measurements since Na+ and Cl− are the ions that contribute most to the electrical conductivity of a standard physiological solution. The system was validated with epithelial monolayers of raTAL and NRK‐52E cells that were characterized electrophysiologically for 5 days under different flow conditions (i.e., apical perfusion, basal, or both). In addition, apical to basal chemical gradients of NaCl (140/70 and 70/140 mM) were imposed in order to demonstrate the feasibility of this methodology for quantifying and monitoring in real time the transepithelial reabsorption of NaCl, which is a primary function of the renal tubule.

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Section: Resultsmentioning

confidence: 99%

“…Concurrently, most of the Cl − is reabsorbed paracellularly due to the generated electrochemical gradient, although Cl − channels and Cl − -coupled transporters also contribute to Cl-reabsorption (Planelles, 2004). NRK-52E cell line is derived from PT and express Na + /glucose cotransporter (Dong, Chen, He, Yang, & Zhang, 2009).…”

Section: Yeste Et Almentioning

confidence: 99%

A perfusion chamber for monitoring transepithelial NaCl transport in an in vitro model of the renal tubule

Yeste

Martínez-Gimeno

Illa

et al. 2018

Biotech & Bioengineering

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“…Some earlier studies suggested or applied bioreactors where renal cells were also seeded on the outer surfaces of HFM . Probably some of the specific advantages of using this configuration were not obvious in these studies, and this might be one reason why this configuration was not widely adopted.…”

Section: Discussionmentioning

confidence: 99%

A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

Kandasamy

Tasnim

et al. 2013

J Cellular Molecular Medi

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Treatment with bioartificial kidneys had beneficial effects in animal experiments and improved survival of critically ill patients with acute kidney injury in a Phase II clinical trial. However, a Phase II b clinical trial failed. This and other results suggested various problems with the current design of bioartificial kidneys. We propose a novel design to improve various properties of device, including haemocompatibility and cell performance. An important feature of the novel design is confinement of the blood to the lumina of the hollow fibre membranes. This avoids exposure of the blood to the non-haemocompatible outer surfaces of hollow fibre membranes, which usually occurs in bioartificial kidneys. We use these outer surfaces as substrate for cell growth. Our results show that commercial hollow fibre membranes can be directly applied in the bioreactor when human primary renal proximal tubular cells are grown in this configuration, and no coatings are required for the formation of robust and functional renal epithelia. Furthermore, we demonstrate that the bioreactor unit produces significant amounts of interleukins. This result helps to understand the immunomodulatory effects of bioartificial kidneys, which have been observed previously. The novel bioartificial kidney design outlined here and the results obtained would be expected to improve the safety and performance of bioartificial kidneys and to contribute to a better understanding of their effects.

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“…Tsuruoka et al reported a renal assist device (RAD) using an immortalized proximal tubular cell line for digoxin and doxorubicin removal [107]. More commonly, the RAD was developed to assess the differentiated tubular functions such as water and glucose reabsorption as well as vitamin D activation [108][109][110][111]. RAD normally consisted of a hollow fiber reactor and two circulations of culture medium (Fig.…”

Section: Membrane-based Cell Model In Renal Excretion Of Drugsmentioning

confidence: 99%

Polymer Membrane and Cell Models for Drug Discovery

Shen¹,

Zhang²,

Zhang³

et al. 2012

CCHTS

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This paper reviews the functional polymer membrane and membrane based cell drug evaluation models for drug discovery. Based on the characteristics of biological membranes in vivo, chemical modification methods of synthetic membrane, including blending and surface modification are explored to mitigate the membrane fouling and improve biocompatibility. Different membrane-based cell models used in drug investigation and related trouble shooting are analyzed in detail. Specific attention is given to the current studies on ADME/Tox of drugs using membrane-based in vitro models of cells including Caco-2, hepatocytes or renal cells, which can be used to evaluate the feasibility of polymer membrane in drug investigation. The progress toward solving present bottlenecks of the facilitated cell models are supposed to provide great benefits to drug discovery in pharmaceutical industry.

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Construction of bioartificial renal tubule assist device In Vitro and its function of transporting sodium and glucose

Cited by 5 publications

References 21 publications

A perfusion chamber for monitoring transepithelial NaCl transport in an in vitro model of the renal tubule

A perfusion chamber for monitoring transepithelial NaCl transport in an in vitro model of the renal tubule

A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

Polymer Membrane and Cell Models for Drug Discovery

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