Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map

Abstract

Linkage maps of the sweet cherry cultivar 'Emperor Francis' (EF) and the wild forest cherry 'New York 54' (NY) were constructed using primarily simple sequence repeat (SSR) markers and gene-derived markers with known positions on the Prunus reference map. The success rate for identifying SSR markers that could be placed on either the EF or NY maps was only 26% due to two factors: a reduced transferability of other Prunusspecies-derived markers and a low level of polymorphism in the mapping parents. To increase marker density, we developed four cleaved amplified polymorphic sequence markers (CAPS), 19 derived CAPS markers, and four insertion-deletion markers for cherry based on 101 Prunus expressed sequence tags. In addition, four gene-derived markers representing orthologs of a tomato vacuolar invertase and fruit size gene and two sour cherry sorbitol transporters were developed. To complete the linkage analysis, 61 amplified fragment length polymorphism and seven sequence-related amplified polymorphism markers were also used for map construction. This analysis resulted in the expected eight linkage groups for both parents. The EF and NY maps were 711.1 cM and 565.8 cM, respectively, with the average distance between markers of 4.94 cM and 6.22 cM. A total of 82 shared markers between the EF and NY maps and the Prunus reference map showed that the majority of the marker orders were the same with the Prunus reference map suggesting that the cherry genome is colinear with that of the other diploid Prunus species.

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

“…Genomic co-linearity and marker transferability has been demonstrated within members of the Rosaceae (Joobeur et al, 1998;Dirlewanger et al, 2004aDirlewanger et al, , 2004bLambert et al, 2004;Dondini et al, 2007;Olmstead et al, 2008;Clarke et al, 2009;Cabrera et al, 2009). In 2009, a set of onserved Rosaceae genebased sequences corresponding to single copy Arabidopsis genes was developed (Cabrera et al, 2009).…”

Fruit size QTL analysis of an F1 population derived from a cross between a domesticated sweet cherry cultivar and a wild forest sweet cherry

“…EF is a large (~8 g) cultivated cherry, while NY is a wild forest cherry of northern European origin, termed "mazzard," that has very small fruits (~2 g). The crosses were carried out in the spring of 2001 (Olmstead et al 2008), and seedling progenies were planted in the field in 2002 at Michigan State University's Clarksville Horticultural Experiment Station in Clarksville, MI, USA. The seedling trees together with their parents were planted at a spacing of 1.5 m within a row and 6.1 m between rows.…”

“…In such a population, markers heterozygous in one parent and homozygous in the other parent will segregate as a test cross with a segregation ratio of 1:1, while dominant markers heterozygous in both parents will segregate as a F 2 cross with a segregation ratio of 3:1. In the previous study (Olmstead et al 2008), a total of 197 markers, including 102 simple sequence repeat (SSR) markers, 61 amplified fragment-length polymorphism markers, 27 gene-derived markers, and seven sequence-related amplified polymorphism markers, were used to construct linkage maps with this mapping population. The previous study (Olmstead et al 2008) generated eight linkage groups for each parent.…”

“…These investigations include sour cherry (Prunus cerasus L.; Wang et al 2000), peach (Yamamoto et al 2002;Dirlewanger et al 2002;Etienne et al 2002;Quilot et al 2004), and apple (King et al 2001;Liebhard et al 2003;Kenis et al 2008). In sweet cherry, no QTL studies have previously been reported as comprehensive genetic maps have only recently been constructed (Olmstead et al 2008;Clarke et al 2009). …”

Genetic diversity in wild sweet cherries (Prunus avium) in Turkey revealed by SSR markers

“…In sweet cherries, microsatellites have been used for germplasm characterization (Lacis et al, 2009), determination of genetic diversity (Dirlewanger et al, 2002;, germplasm management (Wünsch and Hormaza, 2002), parentage analysis (Schueler et al, 2003), cultivar identification (Xuan et al, 2009;Gulen et al, 2010), and mapping genetic linkage (Olmstead et al, 2008). In Prunus, microsatellites developed in one species have been used in different species, demonstrating their transferability and ability to detect polymorphism (Wünsch, 2009).…”