Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine

Wang, Huanran; Hu, Yonghua; Zhang, Weiwei; Sun, Li

doi:10.1016/j.vaccine.2009.04.023

Cited by 160 publications

(81 citation statements)

References 52 publications

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“…The plate was incubated at 28°C for 2 to 3 days. Pseudomonas fluorescens TSS Wang et al 2009;Zhang et al 2009), an aquaculture pathogen that can infect a number of fish species, and the isolated mercuryresistant strain were cultured at 28°C in 2216E liquid media. Escherichia coli strain Top10 was cultured at 37°C in Luria-Bertani (LB) media.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Zhang

Chen

Liu

2011

Appl Microbiol Biotechnol

127

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The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 μM HgCl 2 . SP1 was also highly resistant to other metals, including CdCl 2 , CoCl 2 , CrCl 3 , CuCl 2 , PbCl 2 , and ZnSO 4 , and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl 2 and the removal of HgCl 2 by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg 2+ to volatile and relatively inert monoatomic Hg 0 vapor, was around 5.0. LD 50 of P. putida SP1 to flounder and turbot was 1.5× 10 9 CFU. Biofilm developed by P. putida SP1 was 1-to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl 2 contamination over a broad range of pH.

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Section: Methodsmentioning

confidence: 99%

“…LD 50 was determined as described by Wang et al (2009) cultured to an OD 600 of 1.0 and suspended in sterilized phosphate-buffered saline (PBS). Healthy flounder and turbot (approximately 11 g) were reared at 20 to 22°C in seawater and fed daily with commercial dry pellets.…”

Section: Determination Of Ld 50 By Experimental Infectionmentioning

confidence: 99%

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Zhang

Chen

Liu

2011

Appl Microbiol Biotechnol

127

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“…No bacterium was detected from the examined fish. Before tissue collection, fish were euthanized with an overdose of tricaine methanesulfonate (Sigma, St. Louis, MO, USA) [33].…”

Section: Fishmentioning

confidence: 99%

Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing

Wang

Gan

Cai

et al. 2016

Fish & Shellfish Immunology

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“…During the experiment the inclusion might have enhanced the production of inhibitory substances such as bacteriocins against the pathogenic organism. Smith and Davey (Smith and Davey, 1993) found reduction in diseases caused by Aeromonas salmonicida in Atlantic salmon after challenged with Pseudomonas fluorescens and Wanga (Wanga et al, 2009) detected strong protection against Aeromonas hydrophila infections in Japanese flounder injected with Pseudomonas fluorescens. A study conducted by Eissa and About (Eissa and About El-Ghiet, 2011) revealed that Oreochromis niloticus fed on incorporated diet with Pseudomonas fluorescens biovar I, II and III resulted in resistance against Aeromonas hydrophila infection.…”

Section: Discussionmentioning

confidence: 99%

Effect of an Indigenous Probiotic (Shewanella algae) Isolated from Healthy Shrimp (Penaeus monodon) Intestine on Clarias gariepinus

Ariole¹,

Eddo²

2016

ija

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The efficacy of Shewanella algae, isolated from healthy shrimp (Penaeus monodon) intestine, as a biocontrol agent against Aeromonas hydrophila infection in Clarias gariepinus was evaluated. Spread plated technique was employed for bacterial isolation. The bacterial isolates were screened for in vitro antimicrobial activity against pathogenic Aeromonas hydrophila using agar well diffusion assay. The most active isolate (B2) was identified using phenotypic and molecular methods. The safety of the indigenous probiotic (B2) and the pathogenicity of the Aeromonas hydrophila on Clarias gariepinus were investigated. Feeding experiment was designed to evaluate the in vivo antibacterial efficiency of the most active strain using three groups of fish (Shewanella algae-supplemented feed group, control infected and control non-infected groups). At the end of the experiment, five fish from each group were randomly selected for haematological and serum biochemical response investigation. The parameters analysed were haemoglobin, packed cell volume (PCV), red blood cell (RBC), white blood cell (WBC), lymphocytes, monocytes, total protein and albumin. A total of four genera belonging to Pseudomonas, Shewanella, Vibrio and Proteus which were isolated from shrimp intestine had antagonistic ability against Aeromonas hydrophila with zone of inhibition of 8.0 ± 0.0 mm, 10.0 ± 0.0 mm, 8.0 ± 0.0 mm and 8.0 ± 0.0 mm respectively. Molecular analysis conducted on the most active isolate (B2) revealed that it is closely related to Shewanella algae strain KJ-W32 gi: 385880930. The virulence test carried out on Clarias gariepinus using Aeromonas hydrophila which was injected intraperitoneally, showed 50% lethal dose value at 6.4 x 104 cfu ml-1. The result from the probiotic trial showed that fish fed with incorporated Shewanella algae strain KJ-W32 exhibited better resistance to pathogenic Aeromonas hydrophila than the control infected fish. The mortality rate during the in vivo challenge experiments observed for Shewanella algaesupplemented feed group, control infected and control non -infected fish groups were 0%, 50% and 5% respectively. Fish fed with supplemented diet showed better haematological and serum biochemical performance than those fed with normal fish feed. The study showed that Shewanella algae strain KJ-W32 is beneficial to Clarias gariepinus. Therefore, this indigenous bacterium could be used as effective biocontrol agent for management of aeromonasis in aquaculture.

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Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine

Cited by 160 publications

References 52 publications

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing

Effect of an Indigenous Probiotic (<i>Shewanella algae</i>) Isolated from Healthy Shrimp (<i>Penaeus monodon</i>) Intestine on <i>Clarias gariepinus</i>

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