Construction of an attenuated goatpox virus AV41 strain by deleting the TK gene and ORF8-18

Zhu, Yan; Li, Yiquan; Bai, Bing; Fang, Jinbo; Zhang, Kelong; Yin, Xinghui; Li, Shanzhi; Li, Wenjie; Ma, Yizhen; Cui, Yingli; Wang, Jing; Liu, Xing; Li, Xiao; Sun, Lili; Jin, Ningyi

doi:10.1016/j.antiviral.2018.07.008

Cited by 15 publications

(8 citation statements)

References 36 publications

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“…For the sequential insertion of two or more mutations (deletions/insertions of genes) into the poxvirus genome by homologous recombination, the selective marker gene must be removed after each recombination. For this purpose, the isolation of recombinant viruses can be carried out under conditions of transient dominant selection [ 26 ] or using the Ce/Loxp system [ 27 ]. To isolate recombinant SPPVs, the method of transient dominant selection was used.…”

Section: Discussionmentioning

confidence: 99%

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

Chervyakova¹,

Tailakova²,

Kozhabergenov³

et al. 2021

Microorganisms

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Capripoxviruses with a host range limited to ruminants have the great potential to be used as vaccine vectors. The aim of this work was to evaluate attenuated sheep pox virus (SPPV) vaccine strain NISKHI as a vector expressing several genes. Open reading frames SPPV020 (ribonucleotide kinase) and SPPV066 (thymidine kinase) were selected as sites for the insertion of foreign genes. Two integration plasmids with expression cassette were designed and constructed. Recombinant SPPVs expressing an enhanced green fluorescent protein (EGFP) (rSPPV(RRΔ)EGFP and rSPPV(TKΔ)EGFP), Foot-and-mouth disease virus capsid protein (VP1), and Brucella spp. outer membrane protein 25 (OMP25) (rSPPV(RRΔ)VP1A-(TKΔ)OMP25) were generated under the transient dominant selection method. The insertion of foreign genes into the SPPV020 and SPPV066 open reading frames did not influence the replication of the recombinant viruses in the cells. Successful foreign gene expression in vitro was assessed by luminescent microscopy (EGFP) and Western blot (VP1 and OMP25). Our results have shown that foreign genes were expressed by rSPPV both in permissive (lamb testicles) and non-permissive (bovine kidney, saiga kidney, porcine kidney) cells. Mice immunized with rSPPV(RRΔ)VP1A-(TKΔ)OMP25 elicited specific antibodies to both SPPV and foreign genes VP1 and OMP25. Thus, SPPV NISKHI may be used as a potential safe immunogenic viral vector for the development of polyvalent vaccines.

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Section: Discussionmentioning

confidence: 99%

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

Chervyakova¹,

Tailakova²,

Kozhabergenov³

et al. 2021

Microorganisms

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“…The vaccine also induced the production of neutralizing antibodies and GTPV-specific antibodies, as well as the release of IFN-γ in goats. Hence, removal of nonessential genes that are linked to apoptosis inhibition and immune modulation is considered as a factor that may improve the efficacy of the vaccine [123].…”

Section: Gtpv-av41mentioning

confidence: 99%

NF-κB as an Important Factor in Optimizing Poxvirus-Based Vaccines against Viral Infections

Struzik

Szulc-Dąbrowska

2020

Pathogens

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Poxviruses are large dsDNA viruses that are regarded as good candidates for vaccine vectors. Because the members of the Poxviridae family encode numerous immunomodulatory proteins in their genomes, it is necessary to carry out certain modifications in poxviral candidates for vaccine vectors to improve the vaccine. Currently, several poxvirus-based vaccines targeted at viral infections are under development. One of the important aspects of the influence of poxviruses on the immune system is that they encode a large array of inhibitors of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which is the key element of both innate and adaptive immunity. Importantly, the NF-κB transcription factor induces the mechanisms associated with adaptive immunological memory involving the activation of effector and memory T cells upon vaccination. Since poxviruses encode various NF-κB inhibitor proteins, before the use of poxviral vaccine vectors, modifications that influence NF-κB activation and consequently affect the immunogenicity of the vaccine should be carried out. This review focuses on NF-κB as an essential factor in the optimization of poxviral vaccines against viral infections.

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“…Other insertion sites such as the IL-10 homolog gene (127) and interferon-gamma receptor-like gene have also been used as insertion sites (128). Deletion of the TK as well as open reading frames 8-18 was demonstrated to further attenuate the AV41 sheeppox vaccine (129). Deletion of the sheeppox-019 kelch like protein gene from a virulent Kazakhstan sheeppox isolate was able to attenuate the virus (130).…”

Section: Generation Of Recombinant Capripoxvirus Vectors Homologous Rmentioning

confidence: 99%

Potential of Using Capripoxvirus Vectored Vaccines Against Arboviruses in Sheep, Goats, and Cattle

Teffera

Babiuk

2019

Front. Vet. Sci.

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Capripoxviruses represent a genus of the poxviridae family under the subfamily chordopoxviriniae; the genus includes three animal virus species that have a devastating impact on sheep, goats, and cattle in Africa, Asia, and most recently Eastern Europe (1-4). The viruses in the genus are sheeppox virus (SPPV), goatpox virus (GTPV), and lumpy skin disease virus (LSDV) which affect sheep, goats, and cattle, respectively (1, 4). Capripoxviruses share 98% sequence similarity between

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Construction of an attenuated goatpox virus AV41 strain by deleting the TK gene and ORF8-18

Cited by 15 publications

References 36 publications

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

Engineering of Recombinant Sheep Pox Viruses Expressing Foreign Antigens

NF-κB as an Important Factor in Optimizing Poxvirus-Based Vaccines against Viral Infections

Potential of Using Capripoxvirus Vectored Vaccines Against Arboviruses in Sheep, Goats, and Cattle

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