Construction of a Xylan-Fermenting Yeast Strain through Codisplay of Xylanolytic Enzymes on the Surface of Xylose-Utilizing Saccharomyces cerevisiae Cells

Abstract

Hemicellulose is one of the major forms of biomass in lignocellulose, and its essential component is xylan. We used a cell surface engineering system based on ␣-agglutinin to construct a Saccharomyces cerevisiae yeast strain codisplaying two types of xylan-degrading enzymes, namely, xylanase II (XYNII) from Trichoderma reesei QM9414 and ␤-xylosidase (XylA) from Aspergillus oryzae NiaD300, on the cell surface. In a high-performance liquid chromatography analysis, xylose was detected as the main product of the yeast strain codisplaying XYNII and XylA, while xylobiose and xylotriose were detected as the main products of a yeast strain displaying XYNII on the cell surface. These results indicate that xylan is sequentially hydrolyzed to xylose by the codisplayed XYNII and XylA. In a further step toward achieving the simultaneous saccharification and fermentation of xylan, a xylan-utilizing S. cerevisiae strain was constructed by codisplaying XYNII and XylA and introducing genes for xylose utilization, namely, those encoding xylose reductase and xylitol dehydrogenase from Pichia stipitis and xylulokinase from S. cerevisiae. After 62 h of fermentation, 7

Characterization of Enzymes Associated with Degradation of Insoluble Fiber of Soybean Curd Residue by Bacillus subtilis

“…RB14-CS may produce other enzymes such as -D-xylosidase for this reaction. In recent years, biomass containing hemicellulose, such as agricultural and forestry residues, waste paper, and industrial wastes, has been recognized as inexpensive and abundantly available sources of sugar (Katahira et al, 2004). Since the production of iturin A by RB14-CS in soybean curd residue was almost equivalent to that when glucose was used as carbon source, the utilization of soybean curd residue will be one possible nutrient in peptide production.…”

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

“…la Grange et al, (2001) expressed β-xylosidase and xylanase II genes extracellularly, and the resultant recombinant yeast strain was able to convert birchwood xylan to short-chain xylo-oligomers. In another work, β-xylosidase and xylanase II genes were co-displayed on the cell-surface of S.cerevisiae (Katahira et al, 2004). In both of these studies, xylose was the major end product.…”

“…cellulases, hemicellulases, β-D-glucosidases) as well as xylose-utilizing and arabinose-utilizing enzymes from various species have been introduced into S. cerevisiae, and as a result, some engineered strains that can grow on biomass containing cellulose, hemicellulose, cellobiose, xylose, or arabinose have been reported (Becker et al, 2003;Fujita et al, 2004;Katahira et al, 2004Katahira et al, , 2006van Rooyen et al, 2005;Karhumaa et al, 2006;Van Zyl et al, 2007;den Haan et al, 2007aden Haan et al, ,b, 2013Xu et al, 2009;Tsai et al, 2010;Wen et al, 2010;Yamada et al, 2011;Fan et al, 2012b;Nakatani et al, 2013) (Table 6). van Rooyen et al (2005) designed recombinant strains of S. cerevisiae expressing a β-glucosidase gene (bgl1) from Saccharomycopsis fibuligera.…”

Versatile microbial surface-display for environmental remediation and biofuels production

“…Xylanase breaks down hemicellulose into xylose. Subsequent research from the same group co-displayed XYNII and β-xylosidase (XylA) on the yeast cell surface and intracellularly expressed three xylose fermentation genes (XYL1, XYL2, XKS1) [43]. The yeast strain was capable of producing 7.1 g/l of ethanol from birchwood xylan and the yield was further improved to 30 g/l ethanol with chemically pretreated woodchip [38].…”