Construction of a saturated linkage map for Prunus using an almond×peach F2 progeny

Joobeur, Tarek; Viruel, M. A.; Vicente, Miguel; Jáuregui, B.; Ballester, Jordi; Dettori, Maria Teresa; Verde, Ignazio; Truco, María José; Messeguer, Ramón; Batlle, I.; Quarta, R.; Dirlewanger, Elisabeth; Arús, Pere

doi:10.1007/s001220050988

Cited by 186 publications

(195 citation statements)

References 20 publications

Supporting

Mentioning

181

Contrasting

Unclassified

Order By: Relevance

“…Sweet kernel in almond has been shown to be a monogenic trait and the bitter kernel trait to be recessive (Heppner, 1923(Heppner, , 1926Dicenta and García, 1993;Dicenta et al, 2007). The gene conferring sweetness (Sweet kernel [Sk] gene) belongs to linkage group five (Joobeur et al, 1998;Bliss et al, 2002;Sánchez-Pérez et al, 2007) but the precise localization and function of this maternally inherited gene remains unknown.…”

mentioning

confidence: 99%

Bitterness in Almonds

et al. 2008

View full text Add to dashboard Cite

Bitterness in almond (Prunus dulcis) is determined by the content of the cyanogenic diglucoside amygdalin. The ability to synthesize and degrade prunasin and amygdalin in the almond kernel was studied throughout the growth season using four different genotypes for bitterness. Liquid chromatography-mass spectrometry analyses showed a specific developmentally dependent accumulation of prunasin in the tegument of the bitter genotype. The prunasin level decreased concomitant with the initiation of amygdalin accumulation in the cotyledons of the bitter genotype. By administration of radiolabeled phenylalanine, the tegument was identified as a specific site of synthesis of prunasin in all four genotypes. A major difference between sweet and bitter genotypes was observed upon staining of thin sections of teguments and cotyledons for b-glucosidase activity using Fast Blue BB salt. In the sweet genotype, the inner epidermis in the tegument facing the nucellus was rich in cytoplasmic and vacuolar localized b-glucosidase activity, whereas in the bitter cultivar, the b-glucosidase activity in this cell layer was low. These combined data show that in the bitter genotype, prunasin synthesized in the tegument is transported into the cotyledon via the transfer cells and converted into amygdalin in the developing almond seed, whereas in the sweet genotype, amygdalin formation is prevented because the prunasin is degraded upon passage of the b-glucosidaserich cell layer in the inner epidermis of the tegument. The prunasin turnover may offer a buffer supply of ammonia, aspartic acid, and asparagine enabling the plants to balance the supply of nitrogen to the developing cotyledons.

show abstract

mentioning

confidence: 99%

Bitterness in Almonds

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The genetic map published by Joobeur et al (1998) is considered as the reference map for the genus Prunus. The analysis of 75 F 2 individuals of a cross between almond 'Texas' and peach 'Earlygold' resulted in what is known as the T×E map.…”

Section: Construction Of Genetic Mapsmentioning

confidence: 99%

“…Many of these were developed in peach (Cipriani et al, 1999;Sosinski et al, 2000;Testolin et al, 2000;Aranzana et al, 2002;Dirlewanger et al, 2002) and cherry (Downey and Iezzoni, 2000;Cantini et al, 2001). By collecting all the available information, Aranzana et al (2003b) analyzed 109 SSRs developed in peach and cherry by different research groups and mapped 96 of them on the Prunus reference map (Joobeur et al, 1998). This contribution brought the T×E map to a total of 342 markers, 105 of which were SSRs that had a total length of 522 cM.…”

Section: Construction Of Genetic Mapsmentioning

confidence: 99%

“…Davis' and the melting flesh 'Georgia Belle'. The Pop-DG map covered 818 cM of the peach genome and had a mean distance of 4 cM between markers; this map was colinear with the Prunus reference map (Joobeur et al, 1998;Dirlewanger et al, 2004), with 39 common SSR markers. Likewise, the bin-mapping strategy was used in the T×E population with DNA from the same individuals found in the study by Howad et al (2005), who mapped another 159 markers on the reference map: 59 candidate genes related to maturation, 50 cold-response genes, and 50 new EST-SSRs from the ChillPeach database: their locations were deduced from the Pop-DG map by comparative mapping.…”

Section: Identification Of Qtls and Genesmentioning

confidence: 99%

See 1 more Smart Citation

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

Guajardo¹,

Hinrichsen

Muñoz

2015

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Prunus rootstock is an important choice in optimizing productivity of grafted cultivars. Nevertheless, many Prunus rootstocks are notoriously intolerant to hypoxia which is caused by waterlogging and/or heavy soils. There is no available information to help select Prunus rootstocks that are tolerant to stress conditions such as root hypoxia caused by excess moisture. Information from genetic maps has demonstrated a high level of synteny among Prunus species, and this suggests that they all share a similar genomic structure. It should be possible to identify the genetic determinants involved in tolerance to hypoxia and other traits in Prunus rootstocks by applying methods to identify regions of the genome involved in the expression of important traits; these have been developed mainly in peach which is the model species for the genus. Molecular markers that are tightly linked to major genes would be useful in marker-assisted selection (MAS) to optimize new rootstock selection. This article provides insight on the advances in the development of molecular markers, genetic maps, and gene identification in Prunus, mainly in peach; the aim is to provide a general approach for identifying the genetic determinants of hypoxia stress in rootstocks.

show abstract

“…Its single inheritance was discovered by Heppner (1923), who stated that the bitter phenotype is recessive. This single gene, called Sweet kernel (Sk), has been localized in linkage group 5 (out of eight) of two almond linkage maps (Joobeur et al, 1998;Sánchez-Pérez et al, 2007). Although molecular markers for bitterness have been added close to the Sk locus (Sánchez-Pérez et al, 2010), the sequence of this gene remains unknown.…”

mentioning

confidence: 99%

Prunasin Hydrolases during Fruit Development in Sweet and Bitter Almonds

Sánchez‐Pérez

Belmonte²,

Borch

et al. 2012

Plant Physiology

View full text Add to dashboard Cite

Amygdalin is a cyanogenic diglucoside and constitutes the bitter component in bitter almond (Prunus dulcis). Amygdalin concentration increases in the course of fruit formation. The monoglucoside prunasin is the precursor of amygdalin. Prunasin may be degraded to hydrogen cyanide, glucose, and benzaldehyde by the action of the b-glucosidase prunasin hydrolase (PH) and mandelonitirile lyase or be glucosylated to form amygdalin. The tissue and cellular localization of PHs was determined during fruit development in two sweet and two bitter almond cultivars using a specific antibody toward PHs. Confocal studies on sections of tegument, nucellus, endosperm, and embryo showed that the localization of the PH proteins is dependent on the stage of fruit development, shifting between apoplast and symplast in opposite patterns in sweet and bitter cultivars. Two different PH genes, Ph691 and Ph692, have been identified in a sweet and a bitter almond cultivar. Both cDNAs are 86% identical on the nucleotide level, and their encoded proteins are 79% identical to each other. In addition, Ph691 and Ph692 display 92% and 86% nucleotide identity to Ph1 from black cherry (Prunus serotina). Both proteins were predicted to contain an amino-terminal signal peptide, with the size of 26 amino acid residues for PH691 and 22 residues for PH692. The PH activity and the localization of the respective proteins in vivo differ between cultivars. This implies that there might be different concentrations of prunasin available in the seed for amygdalin synthesis and that these differences may determine whether the mature almond develops into bitter or sweet.

show abstract

Construction of a saturated linkage map for Prunus using an almond×peach F2 progeny

Cited by 186 publications

References 20 publications

Bitterness in Almonds

Bitterness in Almonds

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

Prunasin Hydrolases during Fruit Development in Sweet and Bitter Almonds

Contact Info

Product

Resources

About