Construction of a ratiometric fluorescent probe for visual detection of urea in human urine based on carbon dots prepared from Toona sinensis leaves and 5-carboxyfluorescein

Bei, Yuyang; Arkin, Kamile; Zheng, Yuxin; Ma, Xuesong; Zhao, Jie; Jiang, Huimin; Shang, Qianqian

doi:10.1016/j.aca.2022.340733

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…It is distributed throughout various bodily fluids, such as blood and urine. In human urine, urea concentration typically falls between 275 to 409 mmol/L [1,2]. Therefore, the diagnosis of human physical conditions generally involves analyzing the urea levels in urine.…”

Section: Introductionmentioning

confidence: 99%

Formulation and Synthesis of Vanillin from Clove Oil as a Chemosensor for Urea Detection in Urine

Bhagaskara,

Fadzila,

Sumlang

et al. 2024

J. Kim. Sains Apl.

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Urea levels in urine play an important parameter in diagnosing bodily conditions through liver and kidney examinations. The typical reagent for detecting urea content in urine is para-dimethylaminobenzaldehyde (pDMAB). However, it has a drawback related to the instability of color in the resulting Schiff base compound (pDMAB-urea). In this study, the synthesized vanillin compound derived from clove oil serves as the foundational material for a urea chemosensor based on the colorimetric concept. The synthesized vanillin was characterized using FTIR, GC-MS, and 1H-NMR. The formulation of the vanillin compound as a sensor was conducted by assessing the suitable solvent, determining the optimal mass of vanillin, and evaluating the acid-base conditions of the sensor formulation system both qualitatively and quantitatively. Subsequently, the most effective formulation was selected for detecting urea in urine samples. The synthesis of vanillin yielded a purity level of 95%. The optimal formulation was obtained at an optimum mass of vanilla of 0.75 g in 50 mL of 96% ethanol and 10 mL of 10% NaOH. The color change in the sample was from colorless to greenish yellow (436 nm). The vanillin obtained was applied to urine samples with the best results at a sample dilution level of 10,000×.

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Section: Introductionmentioning

confidence: 99%

Formulation and Synthesis of Vanillin from Clove Oil as a Chemosensor for Urea Detection in Urine

Bhagaskara,

Fadzila,

Sumlang

et al. 2024

J. Kim. Sains Apl.

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show abstract

“…Multifunctional nano drug delivery systems are a hot topic in current research, in which the use of appropriate fluorescent labeling can achieve real-time monitoring of the drug release process. As a mature fluorescent dye, 5-carboxyfluorescein (5-FAM), with its high fluorescence quantum yield, good fluorescence stability, and covalent binding to biomolecules, has great potential to be used as a fluorescent probe in nanodrug-carrying systems. , The connection of 5-FAM can give the nanodrug-carrying system the functions of drug delivery and fluorescence imaging.…”

Section: Introductionmentioning

confidence: 99%

“…As a mature fluorescent dye, 5-carboxyfluorescein (5-FAM), with its high fluorescence quantum yield, good fluorescence stability, and covalent binding to biomolecules, has great potential to be used as a fluorescent probe in nanodrugcarrying systems. 33,34 The connection of 5-FAM can give the nanodrug-carrying system the functions of drug delivery and fluorescence imaging.…”

Section: Introductionmentioning

confidence: 99%

A pH-Responsive Nanoplatform Based on Magnetic Mesoporous Silica Nanoparticles for Enhanced Treatment of Pancreatic Cancer

Qi,

Wang,

Yin

et al. 2023

ACS Appl. Nano Mater.

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A novel urease-assisted ratiometric fluorescence sensing platform based on pH-modulated copper-quenched near-infrared carbon dots and methyl red-quenched red carbon dots for selective urea monitoring

Alanazi,

Alhazzani,

Mostafa

et al. 2024

Microchim Acta

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Construction of a ratiometric fluorescent probe for visual detection of urea in human urine based on carbon dots prepared from Toona sinensis leaves and 5-carboxyfluorescein

Cited by 6 publications

References 29 publications

Formulation and Synthesis of Vanillin from Clove Oil as a Chemosensor for Urea Detection in Urine

Formulation and Synthesis of Vanillin from Clove Oil as a Chemosensor for Urea Detection in Urine

A pH-Responsive Nanoplatform Based on Magnetic Mesoporous Silica Nanoparticles for Enhanced Treatment of Pancreatic Cancer

A novel urease-assisted ratiometric fluorescence sensing platform based on pH-modulated copper-quenched near-infrared carbon dots and methyl red-quenched red carbon dots for selective urea monitoring

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