Construction of a questionnaire to characterize environmental exposures in patients with systemic lupus erythematosus

Hurtado, Carolina; Rojas‐Gualdrón, Diego Fernando; Hernández, Deicy; Díaz, Juan C.; Urrego, Rodrigo; Vásquez-Trespalacios, Elsa María; Pineda, Ricardo; Vásquez, Gloría

doi:10.1016/j.rcreue.2020.07.006

Cited by 1 publication

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“…Given the relevance of environmental exposure in lupus, we wanted to know if environmental events were associated with B cells’ distributions. To answer this question, we constructed an instrument to characterize exposure to relevant environmental factors for SLE patients ( 26 ); this allowed us to classify patients based on exposure to organic solvents. The most used solvent was degreasers for cleaning in patients and HC, followed by ketones such as nail polish remover.…”

Section: Resultsmentioning

confidence: 99%

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Altered B cell phenotype and CD27+ memory B cells are associated with clinical features and environmental exposure in Colombian systemic lupus erythematosus patients

et al. 2022

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BackgroundB lymphocytes are dysregulated in Systemic Lupus Erythematosus (SLE) including the expansion of extrafollicular B cells in patients with SLE of African American ancestry, which is associated with disease activity and nephritis. The population of Colombia has a mixture of European, Native American, and African ancestry. It is not known if Colombian patients have the same B cell distributions described previously and if they are associated with disease activity, clinical manifestations, and environmental exposures.ObjectiveTo characterize B cell phenotype in a group of Colombian Systemic Lupus Erythematosus patients with mixed ancestry and determine possible associations with disease activity, clinical manifestations, the DNA methylation status of the IFI44L gene and environmental exposures.Materials and methodsForty SLE patients and 17 healthy controls were recruited. Cryopreserved peripheral B lymphocytes were analyzed by multiparameter flow cytometry, and the DNA methylation status of the gene IFI44L was evaluated in resting Naive B cells (rNAV).ResultsExtrafollicular active Naive (aNAV) and Double Negative type 2, DN2 (CD27− IgD− CD21− CD11c+) B cells were expanded in severe active patients and were associated with nephritis. Patients had hypomethylation of the IFI44L gene in rNAV cells. Regarding environmental exposure, patients occupationally exposed to organic solvents had increased memory CD27+ cells (SWM).ConclusionaNAV and DN2 extrafollicular cells showed significant clinical associations in Colombian SLE patients, suggesting a relevant role in the disease’s pathophysiology. Hypomethylation of the IFI44L gene in resting Naive B cells suggests that epigenetic changes are established at exceedingly early stages of B cell ontogeny. Also, an alteration in SWM memory cells was observed for the first time in patients exposed to organic solvents. This opens different clinical and basic research possibilities to corroborate these findings and deepen the knowledge of the relationship between environmental exposure and SLE.

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Section: Resultsmentioning

confidence: 99%

“…To determine environmental exposure, we used a questionnaire-based instrument constructed to characterize relevant environmental exposures, designed explicitly for SLE patients, in a previous study ( 26 ). This instrument allowed us to define which patients and HC were occupationally exposed to organic solvents.…”

Section: Methodsmentioning

confidence: 99%