Construction of a patterned hydrogel—fibrous mat bilayer structure to mimic choroid and Bruch's membrane layers of retina

Kömez, Aylin; Baran, Erkan Türker; Erdem, Üzeyir; Hasırcı, Nesrin; Hasırcı, Vasıf

doi:10.1002/jbm.a.35756

Cited by 39 publications

(30 citation statements)

References 38 publications

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“…The biocompatibility of this BCN hydrogel has previously been confirmed both in vitro and in vivo in the chick embryo. Literature data has also repeatedly confirmed the biocompatibility of both gelatin and hyaluronic acid . In this study, we used HDFs as model cells to test if the material supported their attachment and proliferation.…”

Section: Discussionmentioning

confidence: 94%

“…Literature data has also repeatedly confirmed the biocompatibility of both gelatin and hyaluronic acid. 19,20 In this study, we used HDFs as model cells to test if the material supported their attachment and proliferation. We demonstrated that Micrographs of the control and estradiol-releasing hydrogels taken on day 14 of embryonic development demonstrate a significant increase in the mean vessels count with estradiol-releasing hydrogels compared with the controls (n = 6 for each group) HDFs produced significantly more ECM when grown on the estradiol-releasing hydrogels as compared with plain hydrogels.…”

Section: Discussionmentioning

confidence: 99%

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An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

Mangır

Eke

Hasırcı

et al. 2019

Neurourology and Urodynamics

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Introduction and Objectives Soft tissue interposition (STI) using local and/or regional flaps is often necessary in urogenital reconstruction to stimulate wound healing and prevent recurrence. Harvesting STI flaps can cause donor site morbidity and may not be available in some patients. In this study, we designed estradiol (E2) releasing hydrogel that could be used as an alternative to a STI flap and to investigate its ability to stimulate tissue production and angiogenesis. Materials and Methods A hydrogel was constructed by crosslinking a solution of estradiol, methacrylated gelatin (15%, w/v), and methacrylated hyaluronic acid (1%, w/v). The release of estradiol was measured using a UV‐spectrophotometer (λmax = 220 nm). Angiogenesis was evaluated by an ex ovo chicken embryo chorioallantoic membrane (CAM) assay. Results Estradiol was gradually released from the hydrogel over 21 days. The hydrogels could be easily manipulated with surgical forceps without any deformation. The hydrogels significantly increased collagen production of human dermal fibroblasts (HDFs). Scanning electron microscopic examination demonstrated that HDFs produced significantly more extracellular matrix (ECM) on estradiol releasing hydrogels compared with the controls. Estradiol releasing hydrogels doubled the number of blood vessels growing toward the hydrogel compared with the controls (vasculogenic index, 59.6 [±6.4] and 25.6 [±4.0], respectively; [P < 0.05]). Conclusion We present a proangiogenic, degradable hydrogel that can be used as an off‐the‐shelf available substitute to traditional STI flaps. This is achieved by using estradiol as a potent stimulator of new tissue production and new blood vessel formation.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

Mangır

Eke

Hasırcı

et al. 2019

Neurourology and Urodynamics

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“…Until recently, it was believed that scaffolds simulated the extracellular matrix (EMC) in the regeneration of retina, and served as a support for cell migration, adhesion, and morphology only [16][17][18]. Emphasis was on loaded materials and morphology construction to develop biocompatible and biodegradable scaffolds with appropriate mechanical properties [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Application of Nanowires for Retinal Regeneration

Kharaghani¹,

Tajbakhsh²,

Phan³

et al. 2020

Regenerative Medicine

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Nanowires aim at developing advanced architectures are gaining popularity for damaged neural systems. The retina with a complicated structure is an essential part of our visual nervous system. Any disorder inside retina could lead to blindness due to irregularity in transferring neural signals to the brain. In recent years, the emergence of nanostructures, as well as nanowires, has provided a viable means for enhancing the regeneration of retinal. Nanowires with the ability to sense light and converting it to the electrical signals simulate the extracellular electrical properties, which are the newest nanostructures for the retinal applications. The different structure of nanowires has been examined in vitro, and several others are undergoing in vivo for vision recovery. Among the structures, core-shell nanowires and functionalized nanowires with gold nanoparticles attract the attention for the regeneration of retinal neural systems. Herein, subsequently provide an introduction to the anatomy of the retina, and retinal disorders, the latest progress in the regeneration of retina and vision using nanowires will be reviewed. Also, the different structures, including core-shell and functionalized nanowires with nanoparticles, will be examined. Eventually, the point of view and perspective of applying nanowire in retinal regeneration will be offered.

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“…Collagen type I was isolated from Sprague-Dawley rat tails as described by earlier studies. (20,21) For the isolation steps, briefly, tendons from rat tails were dissolved in cold acetic acid (0.5 ᴍ), filtered, dialyzed against phosphate buffer, and centrifuged. The collagen pellet was dissolved in acetic acid (0.15 ᴍ), precipitated with NaCl solution (5% w.v -1 ), dialysis and centrifugation steps were repeated.…”

Section: Introductionmentioning

confidence: 99%

A 2-Compartment Bone Tumor Model for Testing the Efficacy of Cancer Drugs

Kömez

Büyüksungur²,

Antmen³

et al. 2019

Preprint

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We produced a three dimensional (3D) bone tumor model (BTM) to study the interactions between healthy and tumor cells in a tumor tissue microenvironment, migration of the tumor cells and the efficacy of an anticancer drug, Doxorubicin, for personalized medicine applications.The model consisted of two compartments: (a) a healthy bone tissue mimic, poly(lactic acid-coglycolic acid) (PLGA)/beta-tricalcium phosphate (β-TCP) sponge that was seeded with human fetal osteoblastic cells (hFOB) and human umbilical vein endothelial cells (HUVECs), and (b) a tumor mimic, a lyophilized collagen sponge that was seeded with human osteosarcoma cells (Saos-2). The tumor component was introduced to a central cavity created in the healthy bone mimic and together they constituted the total 3D model (3D-BTM). The scaffolds were characterized by determining their mechanical properties, studying their topography and stability with compression tests, microCT, SEM, confocal microscopy and gravimetry. Porosities of the sponges were determined from µCT data as 96.7% and 86% for PLGA/TCP and collagen sponges, respectively. The average diameters of the pores were measured by using ImageJ (NIH, USA) as 199±52 µm for PLGA/TCP and 50-150 μm for collagen scaffolds. Young' modulus of the PLGA/TCP and collagen sponges were determined as 4.76MPa and 140kPa, respectively.Cells seeded on the two sponges were studied independently and together on the BTM. Cell proliferation, morphology, calcium phosphate forming capacity and ALP production were studied on both healthy bone and tumor mimics. All types of cells showed cellular extensions and spread on and in the scaffolds indicating good cell-material interactions. Angiogenic developments in BTM were studied along with migration of cells between the components with immunocytochemistry, SEM, microCT, qRT-PCR and agarose gel electrophoresis. Confocal microscopy showed that a direct contact was established between the cells present in different parts of the BTM; and the HUVEC cells within the healthy bone mimic were observed to migrate into the tumor mimic. This was confirmed by the increase in the levels of angiogenic factors VEGF, bFGF, and IL-8 in the tumor component. The IC50 of Doxorubicin on Saos-2 cells was determined as 0.1876 µg.mL -1 . Doxorubicin was administered to the BTM at 2.7 µg.mL -1 concentration and after allowing one day for interaction, the cell number was determined withAlamar Blue cell viability test as 7-fold lesser compared to 24 h earlier. Apoptosis of the osteosarcoma cells was measured by caspase-3 enzyme activity assay. These results demonstrate the suitability of the 3D BTM model for use in the investigation of activities and migrations of cells in a tumor tissue. These will be very useful in studying metastatic capabilities of cells in addition to personalized drug treatments.3

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Construction of a patterned hydrogel—fibrous mat bilayer structure to mimic choroid and Bruch's membrane layers of retina

Cited by 39 publications

References 38 publications

An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition

Application of Nanowires for Retinal Regeneration

A 2-Compartment Bone Tumor Model for Testing the Efficacy of Cancer Drugs

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