Construction of a novel<scp>DNA</scp>vaccine candidate targeting F gene of genotype<scp>VII</scp>Newcastle disease virus and chicken<scp>IL</scp>‐18 delivered by<i>Salmonella</i>

Gao, Xin; Xu, Ke; Yang, Gui; Shi, Chunwei; Huang, Haibin; Wang, J.; Yang, Wentao; Liu, J.; Liu, Q.; Kang, Yuan-Huan; Jiang, Yanlong; Wang, C.

doi:10.1111/jam.14228

Cited by 10 publications

(13 citation statements)

References 42 publications

(81 reference statements)

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“…Thus, our work demonstrates for the first time that EP can effectively improve the efficacy of NDV DNA vaccines by enhancing the immune response. Other delivery methods, including the use of liposomes, nano-chitosan or Salmonella spp as a vector, have been used to improve NDV DNA vaccines [7,11]. A DNA prime-protein booster strategy can further increase the protection rate [14].…”

Section: Discussionmentioning

confidence: 99%

“…Using complement degradation component 3 as an adjuvant has been shown to increase the production of IgG and IgA induced by the F protein DNA vaccine, accompanied by an increased protection rate [39]. Inoculation with IL-18 and F protein together with Salmonella Typhimurium as a vector by the oral route has been shown to increase lymphocyte proliferation, but it does not promote antibody production or provide sufficient protection [7]. Viral shedding was not measured in either of the above-mentioned studies, and therefore, the impact of these approaches on shedding is not known.…”

Section: Discussionmentioning

confidence: 99%

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Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Xie

et al. 2020

Arch Virol

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Newcastle disease (ND), caused by virulent Newcastle disease virus (NDV) strains, has been one of the most problematic diseases affecting the poultry industry worldwide. Conventional vaccines provide effective protection for birds to survive ND outbreaks, but they may not completely suppress NDV shedding. NDV strains circulate on farms for a long time after the initial infection and cause potential risks. A new vaccine with fast clearance ability and low viral shedding is needed. In this study, we used interleukin-12 (IL-12) as an adjuvant and electroporation (EP) as an advanced delivery system to improve a DNA vaccine candidate. The fusion (F) protein gene from an NDV strain of the prevalent genotype VII.1.1 was cloned to prepare the vaccine. Chickens immunized with the F gene DNA vaccine co-delivered with an IL-12-expressing plasmid DNA showed higher neutralizing antibody levels and stronger concanavalin-A-induced lymphocyte proliferation than those treated with the F gene DNA vaccine alone. The co-delivered vaccine provided 100% protection, and less viral shedding and a shorter release time were observed in challenged chickens than when the F gene DNA vaccine was administered alone. The use of F gene DNA combined with IL-12 delivered by electroporation is a promising approach for vaccination against ND. Abbreviations APC Antigen-presenting cell CEF Chicken embryo fibroblast chIL-12 Chicken IL-12 DMEM Dulbecco's modified Eagle'

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Xie

et al. 2020

Arch Virol

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“…Flow cytometry analysis of Lp-12-triggered T cell responses after vaccination. a The single cells in peripheral-blood were prepared as described and subjected to flow cytometry assay gating was done according to [57]. b Panels representing CD3 + CD4 + T cells for each group.…”

Section: Figmentioning

confidence: 99%

“…Single-cell suspensions from blood were prepared using a peripheral-blood lymphocyte separation kit (Solarbio, Beijing, China), and flow cytometry was performed to detect the percentages of CD3 + CD4 + , CD3 + CD8 + T-cells [57]. The isolated cells (1 × 10 6 ) were incubated with FITC-conjugated anti-chicken CD3 and PE-conjugated anti-chicken CD4 or CD8 (SouthernBiotech) monoclonal antibodies, respectively.…”

Section: Flow Cytometrymentioning

confidence: 99%

Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens

et al. 2020

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Background: Avian coccidiosis posts a severe threat to poultry production. In addition to commercial attenuated vaccines, other strategies to combat coccidiosis are urgently needed. Lactobacillus plantarum has been frequently used for expression of foreign proteins as an oral vaccine delivery system using traditional erythromycin resistance gene (erm). However, antibiotic selection markers were often used during protein expression and they pose a risk of transferring antibiotic resistance genes to the environment, and significantly restricting the application in field production. Therefore, a food-grade recombinant L. plantarum vaccine candidate would dramatically improve its application potential in the poultry industry. Results:In this study, we firstly replaced the erythromycin resistance gene (erm) of the pLp_1261Inv-derived expression vector with a non-antibiotic, asd-alr fusion gene, yielding a series of non-antibiotic and reliable, food grade expression vectors. In addition, we designed a dual-expression vector that displayed two foreign proteins on the surface of L. plantarum using the anchoring sequences from either a truncated poly-γ-glutamic acid synthetase A (pgsA′) from Bacillus subtilis or the L. acidophilus surface layer protein (SlpA). EGFP and mCherry were used as marker proteins to evaluate the surface displayed properties of recombinant L. plantarum strains and were inspected by western blot, flow cytometry and fluorescence microscopy. To further determine its application as oral vaccine candidate, the AMA1 and EtMIC2 genes of E. tenella were anchored on the surface of L. plantarum strain. After oral immunization in chickens, the recombinant L. plantarum strain was able to induce antigen specific humoral, mucosal, and T cell-mediated immune responses, providing efficient protection against coccidiosis challenge. Conclusions:The novel constructed food grade recombinant L. plantarum strain with double surface displayed antigens provides a potential efficient oral vaccine candidate for coccidiosis.

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“…Cytokines play a crucial role in controlling the immune response. Previous studies have indicated that cytokines such as chicken interferon gamma (IFN-γ) [ 6 ], interferon α (IFN-α) [ 7 ], interleukin-1β (IL-1β) [ 8 , 9 ], interleukin-2 (IL-2) [ 10 ], interleukin-18 (IL-18) [ 11 , 12 , 13 , 14 ], interleukin-6 (IL-6) [ 15 ], interleukin-4 (IL-4) [ 16 ], and interleukin-15 (IL-15) could be promising adjuvants, due to their safety and high efficiency [ 17 ]. The co-administration of antigens with cytokines is a promising strategy to reduce the side-effects of adjuvants, as well as to enhance humoral and cell-mediated immune responses to promote vaccine efficacy.…”

Section: Introductionmentioning

confidence: 99%

The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by Lactobacillus plantarum NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens

et al. 2020

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Interleukin-17B (IL-17B) is a protective cytokine of the IL-17 family and plays an essential role in the regulation of mucosal inflammation. However, little is known about the role of IL-17B in the control of viral infections. In this study, a recombinant Lactobacillus plantarum, designated as NC8-ChIL17B, was constructed to express the chicken IL-17B (ChIL-17B) gene. The recombinant ChIL17B (rChIL17B) protein was about 14 kDa and was anchored to the surface of NC8 cells. In vitro, it was found that the rChIL17B protein inhibited the proliferation of the infectious bronchitis virus (IBV) through activation of nuclear factor kappa B (NF-κB) and the JAK (Janus kinase)-STAT (signal transducers and activators of transcription) signaling. Moreover, to evaluate the immunoadjuvant activities of NC8-ChIL17B, 40 three-day-old specific pathogen-free (SPF) chickens were divided into four groups. Three groups were orally vaccinated with fresh NC8, NC8-ChIL17B, and phosphate buffered saline (PBS), along with the infectious bronchitis virus vaccine, and the other group was the PBS-negative control. The results of the IBV-specific antibody titer and the concentration of the cytokines IL-2, IL-4, IL-6, and interferon gamma (IFN-γ) in sera, as well as the concentration of secretory immunoglobulin A (sIgA) in the tracheal and small intestinal mucosa, the number of cluster of differentiation 4 positive (CD4+) and cluster of differentiation 8 positive (CD8+) T cells in the blood, and the expression of immune-related genes all indicated that NC8-ChIL17B efficiently enhanced the humoral and cellular immune responses to IBV vaccine. Moreover, the viral loads in the NC8-ChIL17B- and IBV-vaccinated group were significantly lower than in the control groups, suggesting a significant promotion of the immunoprotection of IBV vaccination against the virulent IBV strain. Therefore, ChIL-17B is a promising, effective adjuvant candidate for chicken virus vaccines.

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Construction of a novelDNAvaccine candidate targeting F gene of genotypeVIINewcastle disease virus and chickenIL‐18 delivered bySalmonella

Cited by 10 publications

References 42 publications

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens

The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by Lactobacillus plantarum NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens

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