Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21

Maidin, Mohd Shawal Thakib; Song, Adelene Ai Lian; Jalilsood, Tannaz; Sieo, Chin Chin; Yusoff, Khatijah; Rahim, Raha Abdul

doi:10.1016/j.plasmid.2014.05.003

Cited by 11 publications

(8 citation statements)

References 32 publications

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“…In the expression vectors for Lactobacillus , both constitutive promoters—such as P pgm (phosphoglycerate mutase promoter), P ldhl (lactate dehydrogenase promoter), or P slpA (promoter of the gene encoding the S-layer of the protein SlpA)—and inducible promoters are used. One of them is the promoter of a gene coding for a heat shock protein from Enterococcus faecium ; its activity has been analyzed in cells of L. plantarum (Maidin et al 2014 ). Recent global analysis of lactobacilli transcriptomes, or the investigation of genome libraries using various reporter genes, has resulted in identification of promoters induced by specific environmental conditions.…”

Section: Genetic Engineering Tools Used For Cloning Heterologous Genementioning

confidence: 99%

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

Wyszyńska

Kobierecka

Bardowski

et al. 2015

Appl Microbiol Biotechnol

120

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Lactic acid bacteria (LAB) are a diverse group of Gram-positive, nonsporulating, low G + C content bacteria. Many of them have been given generally regarded as safe status. Over the past two decades, intensive genetic and molecular research carried out on LAB, mainly Lactococcus lactis and some species of the Lactobacillus genus, has revealed new, potential biomedical LAB applications, including the use of LAB as adjuvants, immunostimulators, or therapeutic drug delivery systems, or as factories to produce therapeutic molecules. LAB enable immunization via the mucosal route, which increases effectiveness against pathogens that use the mucosa as the major route of entry into the human body. In this review, we concentrate on the encouraging application of Lactococcus and Lactobacillus genera for the development of live mucosal vaccines. First, we present the progress that has recently been made in the field of developing tools for LAB genetic manipulations, which has resulted in the successful expression of many bacterial, parasitic, and viral antigens in LAB strains. Next, we discuss the factors influencing the efficacy of the constructed vaccine prototypes that have been tested in various animal models. Apart from the research focused on an application of live LABs as carriers of foreign antigens, a lot of work has been recently done on the potential usage of nonliving, nonrecombinant L. lactis designated as Gram-positive enhancer matrix (GEM), as a delivery system for mucosal vaccination. The advantages and disadvantages of both strategies are also presented.

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Section: Genetic Engineering Tools Used For Cloning Heterologous Genementioning

confidence: 99%

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

Wyszyńska

Kobierecka

Bardowski

et al. 2015

Appl Microbiol Biotechnol

120

View full text Add to dashboard Cite

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“…Various constitutive and inducible expression systems have been developed for L. lactis as screening of promoters using reporter genes such as beta-galactosidase are a commonly used strategy in developing novel lactococcal expression systems [7, 8]. P45 and P32 are commonly used as constitutive lactococcal promoters, but stronger promoters are still being discovered and developed to improve the system [9].…”

Section: The Lactococcal Molecular Toolboxmentioning

confidence: 99%

A review on Lactococcus lactis: from food to factory

et al. 2017

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Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features of this gram-positive lactic acid non-colonizing gut bacteria include its generally recognized as safe (GRAS) status, probiotic properties, the absence of inclusion bodies and endotoxins, surface display and extracellular secretion technology, and a diverse selection of cloning and inducible expression vectors. This have made L. lactis a desirable and promising host on par with other well established model bacterial or yeast systems such as Escherichia coli, Salmonella cerevisiae and Bacillus subtilis. In this article, we review recent technological advancements, challenges, future prospects and current diversified examples on the use of L. lactis as a microbial cell factory. Additionally, we will also highlight latest medical-based applications involving whole-cell L. lactis as a live delivery vector for the administration of therapeutics against both communicable and non-communicable diseases.

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“…The plasmid pAR6 from E. faecium , contains a newly characterized heat shock promoter (P hsp ), which was found to regulate the expression of α-crystallin heat shock protein (hsp) in the plasmid [ 156 ]. P hsp was used for construction of a novel Lactobacillus vector pAR1801, which can replicate in L. plantarum and L. lactis [ 156 ].…”

Section: Diversity and Similarity Of Plasmids From Labmentioning

confidence: 99%

Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments

Cui

et al. 2015

IJMS

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Plasmids are widely distributed in different sources of lactic acid bacteria (LAB) as self-replicating extrachromosomal genetic materials, and have received considerable attention due to their close relationship with many important functions as well as some industrially relevant characteristics of the LAB species. They are interesting with regard to the development of food-grade cloning vectors. This review summarizes new developments in the area of lactic acid bacteria plasmids and aims to provide up to date information that can be used in related future research.

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Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21

Cited by 11 publications

References 32 publications

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination

A review on Lactococcus lactis: from food to factory

Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments

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