Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.

Escher, Alan; Kane, D. O'; Lee, J.; Langridge, William H. R.; Szalay, Aladar A.

doi:10.1117/12.962702

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Expression In Eukaryotic Systems1

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1991

1994

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Cited by 4 publications

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“…Moreover, the activity of the fused luciferase in extracts of E. coli is highly dependent on the temperature of cellular growth, whereas the activity of the native enzyme remains relatively constant (49,50). This effect has been proposed to 9.…”

Section: Expression In Eukaryotic Systemsmentioning

confidence: 99%

Molecular biology of bacterial bioluminescence.

Meighen

1991

Microbiological Reviews

490

274

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Section: Expression In Eukaryotic Systemsmentioning

confidence: 99%

Molecular biology of bacterial bioluminescence.

Meighen

1991

Microbiological Reviews

490

274

View full text Add to dashboard Cite

Low‐light image analysis of transgenic organisms using bacterial luciferase as a marker

Langridge

Escher

Wang

et al. 1994

J. Biolumin. Chemilumin.

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Methods for measurement of a novel light-emitting reporter gene system in bacteria, yeast, plant cells, plant tissues and intact plant organs are described. The principle underlying the assay procedures is the bacterial luciferase catalysed oxidation of reduced flavin mononucleotide (FMNH2) in the presence of the ten carbon aldehyde decanal, to yield FMN, decanoic acid, water and a photon of light at 490 nm which can be captured by X-ray film, a photomultiplier tube or, for in vivo measurements, an image-intensifier coupled to a video camera. This light measuring assay system is sensitive, easy to use, inexpensive, does not require radioactivity, and has been used successfully for rapid detection of bacterial transformants, the quantitative measurement of transient and stable gene expression in bacteria and yeast, and in vivo measurement of temporal and spatial gene expression throughout plant and animal development.

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