Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair

Lai, Bi-Qin; Bai, Yu-Rong; Han, Wei-Tao; Bao, Zhang; Liu, Shu; Sun, Jia-Hui; Liu, Jialin; Li, Ge; Zeng, Xiang; Ding, Ying; Ma, Yuan-Huan; Zhang, Ling; Chen, Zhenghong; Wang, Jun; Xiong, Yuan; Wu, Jiadong; Qi, Qi; Xing, Lingyan; Zeng, Yuan-Shan

doi:10.1016/j.bioactmat.2021.10.005

Cited by 22 publications

(19 citation statements)

References 47 publications

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“…Finally, we examined two major extracellular matrix (ECM) proteins at the lesion site—laminin (LN), which enhances neuronal survival and promotes neurite growth, 23 and chondroitin sulfate proteoglycan (CSPG), which inhibits neurite growth and facilitates glial scar formation. LN expression was the highest in the transplanted region in the SCI + L + A + P group (38.87 ± 1.28%), followed by the SCI + L + A (30.89 ± 1.60%) and SCI + L + P (26.64 ± 0.58%) groups, but was only weakly expressed in the SCI group (19.98 ± 0.73%) (Figure 4c,g).…”

Section: Resultsmentioning

confidence: 99%

Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes

Chen

Zhang

et al. 2022

Bioengineering & Transla Med

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Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter‐scale human spinal cord neural tissue (hscNT) construct with human spinal cord neural progenitor cells (hscNPCs) and human spinal cord astrocytes (hscAS) on a linearly ordered collagen scaffold (LOCS). The hscAS promoted hscNPC adhesion, survival and neurite outgrowth on the LOCS, to form a linearly ordered spinal cord‐like structure consisting of mature neurons and glia cells. When transplanted into rats with SCI, the hscNT created a favorable microenvironment by inhibiting inflammation and glial scar formation, and promoted neural and vascular regeneration. Notably, the hscNT promoted neural circuit reconstruction and motor functional recovery. Engineered human spinal cord implants containing astrocytes and neurons assembled on axon guidance scaffolds may therefore have potential in the treatment of SCI.

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Section: Resultsmentioning

confidence: 99%

Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes

Chen

Zhang

et al. 2022

Bioengineering & Transla Med

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“…56 In vitro, NT-3 promotes the neuronal differentiation and formation of synapses from NSC-derived neurons, demonstrating the potency of synaptic transmission. 57,58 Similarly, NGFs have been reported to promote axonal sprouting, guide axon regeneration and myelination after nerve injury and the upregulated NGF at the perilesion site contributing to repair and synaptic plasticity after SCI. [59][60] Furthermore, CNTF is able to promote the survival and axonal growth of neurons and achieved a promyelinating effect in vitro.…”

Section: Biomaterials Loaded With Neurotrophic Factors To Activate En...mentioning

confidence: 99%

Activating Endogenous Neurogenesis for Spinal Cord Injury Repair: Recent Advances and Future Prospects

Yang

et al. 2023

Neurospine

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After spinal cord injury (SCI), endogenous neural stem cells are activated and migrate to the injury site where they differentiate into astrocytes, but they rarely differentiate into neurons. It is difficult for brain-derived information to be transmitted through the injury site after SCI because of the lack of neurons that can relay neural information through the injury site, and the functional recovery of adult mammals is difficult to achieve. The development of bioactive materials, tissue engineering, stem cell therapy, and physiotherapy has provided new strategies for the treatment of SCI and shown broad application prospects, such as promoting endogenous neurogenesis after SCI. In this review, we focus on novel approaches including tissue engineering, stem cell technology, and physiotherapy to promote endogenous neurogenesis and their therapeutic effects on SCI. Moreover, we explore the mechanisms and challenges of endogenous neurogenesis for the repair of SCI.

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“…The mature neurotrophins then bind to their corresponding receptors, the Trk family of receptor tyrosine kinases, and regulate neuronal survival and synaptic plasticity [100,101]. Aberrant expressions of NT-3 and NT-4/5 participate in pathophysiological conditions including motor dysfunction, cognitive decline, stroke, and SCZ [102][103][104][105][106][107].…”

Section: Other Neurotrophinsmentioning

confidence: 99%

The emerging role of furin in neurodegenerative and neuropsychiatric diseases

Zhang

Gao

Bai

et al. 2022

Transl Neurodegener

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Furin is an important mammalian proprotein convertase that catalyzes the proteolytic maturation of a variety of prohormones and proproteins in the secretory pathway. In the brain, the substrates of furin include the proproteins of growth factors, receptors and enzymes. Emerging evidence, such as reduced FURIN mRNA expression in the brains of Alzheimer’s disease patients or schizophrenia patients, has implicated a crucial role of furin in the pathophysiology of neurodegenerative and neuropsychiatric diseases. Currently, compared to cancer and infectious diseases, the aberrant expression of furin and its pharmaceutical potentials in neurological diseases remain poorly understood. In this article, we provide an overview on the physiological roles of furin and its substrates in the brain, summarize the deregulation of furin expression and its effects in neurodegenerative and neuropsychiatric disorders, and discuss the implications and current approaches that target furin for therapeutic interventions. This review may expedite future studies to clarify the molecular mechanisms of furin deregulation and involvement in the pathogenesis of neurodegenerative and neuropsychiatric diseases, and to develop new diagnosis and treatment strategies for these diseases.

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Construction of a niche-specific spinal white matter-like tissue to promote directional axon regeneration and myelination for rat spinal cord injury repair

Cited by 22 publications

References 47 publications

Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes

Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes

Activating Endogenous Neurogenesis for Spinal Cord Injury Repair: Recent Advances and Future Prospects

The emerging role of furin in neurodegenerative and neuropsychiatric diseases

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