Construction of a nanofiber network within 3D printed scaffolds for vascularized bone regeneration

Geng, Meng; Zhang, Qianqian; Gu, Jiani; Jin, Yang; Du, Haibo; Jia, Yating; Zhou, Xiaojun; He, Chuanglong

doi:10.1039/d0bm02058c

Cited by 36 publications

(33 citation statements)

References 48 publications

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“…Some pioneering studies have been attempted to address this issue, such as the use of thermally induced phase separation (TIPS) technique to fabricate nanofiber structures in 3D printed scaffolds to promote cell ingrowth and neo‐tissue formation. [ 5 ] Although the porous structures, as an essential factor in scaffolds, could offer suitable microenvironment for the adhesion, proliferation, and differentiation of cells as well as biomineralization, however, it alone is insufficient which lacks biological cues for guiding angiogenesis and osteogenesis. [ 6 ] Recently, it has been reported that microchannels scaffolds with the tubular structures could promote the invasion of blood vessels.…”

Section: Introductionmentioning

confidence: 99%

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

et al. 2022

Adv Funct Materials

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Microchannel networks within engineered 3D scaffold can allow nutrient exchange and rapid blood vessels formation. However, fabrication of a bone microenvironment‐mimicking scaffold with hierarchical micro/nanofibrous and microchannel structures is still a challenge. Herein, inspired by structural and functional cues of bone remodeling, a microchannel networks‐enriched nanofibrous scaffold by using 3D printing and thermally induced phase separation techniques, which can facilitate cells migration and nutrients transportation, is developed. The customizable vascular‐like structure of polycaprolactone within the nanofibrous gelatin‐silica scaffold is fabricated using 3D‐printed sacrificial templates, while dimethyloxalylglycine (DMOG)‐loaded mesoporous silica nanoparticles (MSNs) located on the scaffold surface and bone forming peptide‐1 (BFP)‐loaded MSNs embedded in the scaffold are implemented for sequential release of DMOG and BFP. The cell experiments show that dual‐drug delivery scaffold (DBM/GP) promotes angiogenesis by stimulating migration, tube formation, and angiogenesis‐related genes/protein expression of endothelial cells, and osteogenesis by promoting osteo‐related genes expression and mineral deposition of osteoblasts. Additionally, DBM/GP scaffold facilitates the angiogenic activity of osteoblasts by activating phosphatidylinositol 3‐kinase/protein kinase B/hypoxia inducible factor‐1α pathway. Furthermore, enhanced vascularization and bone regeneration of DBM/GP scaffold are demonstrated via subcutaneous and skull defect models. Overall, this study reveals that the bone microenvironment‐mimetic dual‐drug delivery scaffold provides a promising strategy for bone defects treatment.

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Section: Introductionmentioning

confidence: 99%

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

et al. 2022

Adv Funct Materials

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103

111

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“…The usual techniques for preparing modified polyesters for artificial bone-substitute materials involve 3D printing [ 51 , 61 , 65 , 66 , 72 , 74 , 75 , 76 ], thermally induced phase separation (TIPS) [ 32 ], salt leaching [ 32 , 69 , 79 ], solvent casting [ 33 , 35 , 77 , 79 ], electrospinning [ 60 , 64 , 70 ], copolymerization [ 31 ], and polycondensation [ 73 ].…”

Section: Orthopedic Applicationsmentioning

confidence: 99%

Special Features of Polyester-Based Materials for Medical Applications

Darie-Niță¹,

Râpă

Frąckowiak

2022

Polymers

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This article presents current possibilities of using polyester-based materials in hard and soft tissue engineering, wound dressings, surgical implants, vascular reconstructive surgery, ophthalmology, and other medical applications. The review summarizes the recent literature on the key features of processing methods and potential suitable combinations of polyester-based materials with improved physicochemical and biological properties that meet the specific requirements for selected medical fields. The polyester materials used in multiresistant infection prevention, including during the COVID-19 pandemic, as well as aspects covering environmental concerns, current risks and limitations, and potential future directions are also addressed. Depending on the different features of polyester types, as well as their specific medical applications, it can be generally estimated that 25–50% polyesters are used in the medical field, while an increase of at least 20% has been achieved since the COVID-19 pandemic started. The remaining percentage is provided by other types of natural or synthetic polymers; i.e., 25% polyolefins in personal protection equipment (PPE).

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“…Besides electrospinning, other techniques, such as TIPS and DIPS, were also used with freeze-drying as hybrid fabrication technologies for TE scaffolds. Geng et al [ 107 ] developed a 3D-printed biodegradable poly (glycerol-co-sebacic acid-co- l -lactic acid-co-polyethylene glycol) (PGSLP)-based scaffold that was internally filled with gelatin nanofibers. They fabricated the nanofibrous structured gelatin/PGSLP (NGP) scaffold employing a thermally induced phase separation (TIPS) technique, and then the macroporous structured gelatin/PGSLP (MGP) scaffold was prepared by directly freeze-drying.…”

Section: Combination Of Solution-based Techniquesmentioning

confidence: 99%

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

et al. 2021

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The fabrication of 3D scaffolds is under wide investigation in tissue engineering (TE) because of its incessant development of new advanced technologies and the improvement of traditional processes. Currently, scientific and clinical research focuses on scaffold characterization to restore the function of missing or damaged tissues. A key for suitable scaffold production is the guarantee of an interconnected porous structure that allows the cells to grow as in native tissue. The fabrication techniques should meet the appropriate requirements, including feasible reproducibility and time- and cost-effective assets. This is necessary for easy processability, which is associated with the large range of biomaterials supporting the use of fabrication technologies. This paper presents a review of scaffold fabrication methods starting from polymer solutions that provide highly porous structures under controlled process parameters. In this review, general information of solution-based technologies, including freeze-drying, thermally or diffusion induced phase separation (TIPS or DIPS), and electrospinning, are presented, along with an overview of their technological strategies and applications. Furthermore, the differences in the fabricated constructs in terms of pore size and distribution, porosity, morphology, and mechanical and biological properties, are clarified and critically reviewed. Then, the combination of these techniques for obtaining scaffolds is described, offering the advantages of mimicking the unique architecture of tissues and organs that are intrinsically difficult to design.

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Construction of a nanofiber network within 3D printed scaffolds for vascularized bone regeneration

Abstract: Three-dimensional (3D) printed scaffolds provide promising perspective in bone tissue engineering. 3D printed scaffolds with micro- and nano-fibrous structure that facilitates the cell adhesion and migration, and combined vascularization and...

Cited by 36 publications

References 48 publications

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration

Special Features of Polyester-Based Materials for Medical Applications

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

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