Construction of a multi-epitope in silico vaccine against Anaplasma Marginale using immunoinformatics approach

Siddiki, Amam Zonaed; Alam, Sabreena; Tithi, Fahmida Alam; Hoque, Syeda Farjana; Sajib, Emran Hossain; Hossen, Farhan Fuad Bin; Hossain, M. A.

doi:10.1016/j.bcab.2023.102706

Cited by 1 publication

(2 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ERRAT program compares non-bonded interactions between various atom types in the protein with those observed in highly refined proteins. (Siddiki et al 2023 ). Based on the ERRAT analysis, the overall quality factor of the vaccine construct was 91.86.…”

Section: Resultsmentioning

confidence: 99%

“…The refined model was further analyzed to evaluate the quality of the final vaccine construct. PROCHECK was utilized to generate a Ramachandran plot by evaluating the stereochemical quality of a protein structure by analyzing the geometry of individual residues and assessing the overall structure (Siddiki et al 2023 ). The protein's Ramachandran plot indicated that the core region contained 91.7% of the amino acids, while 6.1% of the amino acids were situated in the allowed region.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

In silico analysis of virulence factors of Streptococcus uberis for a chimeric vaccine design

Yılmaz Çolak

2024

In Silico Pharmacol.

View full text Add to dashboard Cite

Streptococcus uberis is one of the causative agents of bovine mastitis, which has detrimental effects on animal health and the dairy industry. Despite decades of research, the requirement for effective vaccines against the disease remains unmet. The goal of this study was to create a multi-epitope vaccine using five virulence factors of S. uberis through the reverse vaccinology approach, which has been employed due to its high efficiency and applicability. Plasminogen activator A (PauA), glyceraldehyde-3-phosphate dehydrogenase C (GapC), C5a peptidase, S. uberis adhesion molecule (SUAM), and sortase A (SrtA) were selected for the T cytotoxic (CTL) and B cell epitope analyses as they were extensively studied in S. uberis or other pathogens. Eighteen CTL and ten B cell epitopes that were antigenic, non-toxic, and non-allergenic were selected in order to design a chimeric vaccine candidate that in silico analysis revealed to be potentially immunogenic, non-allergenic, and stable. Molecular docking analysis of the vaccine candidate with Toll-like receptor (TLR) 2 and TLR 4 revealed stable interactions between the candidate and the immune receptors. Meanwhile, the stability of the docked complexes was confirmed using normal mode analysis. Additionally, in silico immune simulation of the vaccine candidate demonstrated the stimulation of primary immune responses, indicating that the chimeric protein can hold promise as a viable vaccine candidate for preventing S. uberis mastitis. Moreover, the current study can provide a background for designing epitope-based vaccines based on the explored epitopes.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%