Construction of a lncRNA-associated competing endogenous RNA regulatory network after traumatic brain injury in mouse

Abstract

Traumatic brain injury (TBI) is a major public health problem worldwide which causes high mortality and disability. Functioning as microRNA (miRNA) sponges, long non-coding RNA (lncRNA) regulates the expression of protein-coding genes in a competing endogenous RNA (ceRNA) network. However, the lncRNA-associated ceRNA in TBI remains unclear. In this study, we processed the raw SRR files of mice cortex samples of sham injury (n = 3) and TBI groups (n = 3) to count files. Then, the expression profiles of lncRNAs and mRNAs were identified, and 86 differentially expressed (DE) lncRNAs and 1201 DEmRNAs between sham and TBI groups were identified. The DEmRNAs were used to perform enrichment analyses. Next, a lncRNA-miRNA-mRNA regulatory ceRNA network was constructed. The network consisted of 23 mRNAs, 5 miRNAs and 2 lncRNAs. The expression alternations of the 5 miRNAs were validated via qRT-PCR. The subnetwork of hub lncRNA Neat1 was extracted. We identified a potential inflammatory associated regulatory axis: Neat1/miR-31-5p/Myd88 axis. The PPI network based on DEmRNA involved in ceRNA network was constructed PPI networks to identify the hub genes. Finally, DElncRNAs and DEmRNAs were selected randomly and validated by qRT-PCR. In conclusion, with the lncRNA-miRNA-mRNA ceRNA network provided above, we can improve our understanding of the regulatory mechanisms and interaction among lncRNAs, miRNAs and mRNAs in TBI process.

Correction to: Construction of a lncRNA-associated competing endogenous RNA regulatory network after traumatic brain injury in mouse

“…This error is corrected in the author list of this Correction article and the original article [ 1 ] has been updated.…”

“…Following publication of the original article [1], the authors identified an error in the affiliations assignment: the affiliation 'Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University' was incorrectly assigned to Yiyu Sun as a second affiliation, while this author is only affiliated to the 'Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University' . This error is corrected in the author list of this Correction article and the original article [1] has been updated.…”

Characterization of the lncRNA-miRNA-mRNA regulatory network to reveal potential functional competing endogenous RNAs in traumatic brain injury

“…Of course, there has been a lot of researches on ceRNAs in other different neurological diseases, such as Alzheimer’s disease (AD) ( Roberts et al, 1652 ), ischemic stroke ( Zhang et al, 2018 ), and spinal cord injury (SCI) ( Gu et al, 2017 ). Previous studies have reported the changes of lncRNA and mRNA in TBI mice ( Zhong et al, 2016 ), as well as the changes of miRNA in rat hippocampus after TBI ( Hu et al, 2012 ) and ceRNA network of mice following TBI ( Wang et al, 2022 ). Though these researchers have made a lot of contributions to TBI research, their research focused on the cortex of mice or the hippocampus of rats.…”