Construction of a highly sensitive NADH sensing platform based on PDDA-rGO nanocomposite modified electrode

Abstract

In this article, we constructed an electrochemical sensor for highly sensitive determination of β-nicotinamide adenine dinucleotide (NADH) using the poly(diallyldimethylammonium chloride)-functionalized reduced graphene oxide nanocomposite modified electrode (PDDA-rGO/GCE). The nanocomposite was characterized by transmission electron microscopy, Fourier transform-infrared spectrometry, ultraviolet-visible absorption spectrometry, cyclic voltammetry, and electrochemical impedance spectroscopy. The experimental results demonstrated that the fabricated sensor exhibited excellent electrocatalytic activity on the oxidation of NADH with a decreased about 200 mV in overpotential and 5.0-fold increment in the oxidation peak current compared to a bare GCE. Applying cyclic voltammetry, a wide linear range from 1.0 × 10 −7 to 2.9 × 10 −3 M with low limit of detection (LOD) of 3.4 × 10 −8 M and high sensitivity of 463.3 μA mM −1 cm −2 were obtained, which was superior to other NADH electrochemical sensors reported previously. Furthermore, the fabricated NADH sensor possessed excellent selectivity, reproducibility, and stability. The oxidation mechanism of NADH at the PDDA-rGO/GCE was also discussed in detail.

Effects of poly(diallyldimethylammonium chloride) addition on the curing kinetics of urea-formaldehyde adhesives for particleboards

“…However, a growing number of studies have shown successful applications of PDDA in other areas. It can be a functional, reducing and stabilizing agent in the preparation of graphene based composites for use in highly selective electrochemical sensors for various substances, such as esculetin [1], 4-chlorophenol [2], paracetamol, and diclofenac [3], β-nicotinamide adenine dinucleotide [4] and levofloxacin [5]. PDDA is used as a dispersive agent to obtain supermagnetic Fe3O4/PDDA nanocomposites [6].…”