Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2

Murai, Hiroshi; Hashimoto, Z.; Sharma, Pramod Kumar; Shimizu, Tomoyuki; Murata, Keiko; Takumi, Shigeo; Mori, Naoki; Kawasaki, Shinji; Nakamura, Chiharu

doi:10.1007/s001220100598

Cited by 64 publications

(42 citation statements)

References 21 publications

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“…Bulked segregant analysis (Michelmore et al 1991), combined with the AFLP technique (Vos et al 1995), has been used as an efficient way to identify molecular markers closely linked to the target genes in barley (Simons et al 1997), rice (Murai et al 2001), sorghum (Wen et al 2002) and potato (Meksem et al 1995). Therefore, we adopted the same approach to identify AFLP markers closely linked to the nud locus of barley in this study.…”

Section: Discussionmentioning

confidence: 98%

Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley

et al. 2003

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The hulled or naked caryopsis character of barley ( Hordeum vulgare L.) is an important trait for edibility and to follow its domestication process. A single recessive gene, nud, controls the naked caryopsis character, and is located on the long arm of chromosome 7H. To develop a fine map around the nud locus efficiently, the HEGS (High Efficiency Genome Scanning) electrophoresis system was combined with amplified fragment length polymorphism (AFLP). From bulked segregant analysis of 1,894 primer combinations, 12 AFLP fragments were selected as linked markers. For mapping, an F(2 )population of 151 individuals derived from a cross between Kobinkatagi (naked type) and Triumph (hulled type) was used. Seven AFLP markers were localized near the nud region. A fine map was developed with one-order higher resolution than before, along with the seven anchor markers. Among the seven linked AFLP markers (KT1-7), KT1, KT2 and KT6 were co-dominant, and the former two were detected for their single-nucleotide polymorphisms (SNPs) in the same length of fragments after electrophoresis with the non-denaturing gels of HEGS. The nud locus has co-segregated with KT3 and KT7, and was flanked by KT2 and KT4, at the 0.3-cM proximal and the 1.2-cM distal side, respectively. Four of these AFLP markers were converted into sequence-characterized amplified region (SCAR) markers, one of which was a dominant marker co-segregating with the nud gene.

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Section: Discussionmentioning

confidence: 98%

Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley

et al. 2003

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“…The combination of the HEGS system and AFLP technique, which uses nondenatured acrylamide gel, discontinuous Tris-Glycine buffer and Vistra Green staining, allows a single person to construct a map in a short period under a reasonable cost. Using this system, high-density maps were constructed in rice (Murai et al 2001;Shimizu et al, personal communication) and Pyricularia oryzae, a causal fungus of rice blast disease (Motomura et al, personal communication).…”

Section: Introductionmentioning

confidence: 99%

Efficient construction of high-density linkage map and its application to QTL analysis in barley

et al. 2003

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Using a High Efficiency Genome Scanning (HEGS) system and recombinant inbred (RI) lines derived from the cross of Russia 6 and H.E.S. 4, a high-density genetic map was constructed in barley. The resulting 1,595.7-cM map encompassed 1,172 loci distributed on the seven linkage groups comprising 1,134 AFLP, 34 SSR, three STS and vrs1 (kernel row type) loci. Including PCR reactions, gel electrophoresis and data processing, 6 months of work by a single person was sufficient for the whole mapping procedure under a reasonable cost. To make an appraisal of the resolution of genetic analysis for the 95 RI lines based on the constructed linkage map, we measured three agronomic traits: plant height, spike exsertion length and 1,000-kernel weight, and the analyzed quantitative trait loci (QTLs) associated with these traits. The results were compared on the number of detected QTLs and their effects between a high-density map and a skeleton map constructed by selected AFLP and anchor markers. The composite interval mapping on the high-density map detected more QTLs than the other analyses. Closely linked markers with QTLs on the high-density map could be powerful tools for marker-assisted selection in barley breeding programs and further genetic analyses including an advanced backcross analysis or a map-based cloning of QTL.

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“…We found that bph4 from Babawee introgressed into the cultivar TN1 behaved as a major recessive gene, whereas it behaved as a major dominant gene when introgressed into KDML105. According to previous studies, recessive resistance genes can behave as dominant genes under different genetic backgrounds or with different insect biotypes (Murai et al 2001). In the present study, we used only one insect population (biotype 4) to screen the F 2 Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The bph2 gene was first found in a cultivar ASD7 and behaved as a recessive gene in a cross of TN1/ASD7 (Athwal et al 1971). However, the studies on the resistance segregation in a large number of F 2 and F 3 progenies from crosses of ASD7/C418 and Tsukushibare/Norin-PL4, respectively confirmed the dominant nature of bph2 (Murai et al 2001, Murata et al 1998.…”

Section: Discussionmentioning

confidence: 99%

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

et al. 2010

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The brown planthopper (BPH) resistance gene bph4 has previously been assigned on the short arm of rice chromosome 6. However, the map position of the gene could not be determined. To detect the bph4 locus, 15 polymorphic simple sequence repeat (SSR) markers covering genetic distance of 0.0-63.4 cM on chromosome 6 were used to survey 15 BPH resistant (R) and susceptible (S) individuals from each of the 95 and 78 F 2 populations derived from crosses of TN1/Babawee and Babawee/KDML105, respectively. One SSR marker, RM586, was associated with the R and S from the F 2 populations. Additional markers surrounding the RM586 locus were examined to define the location of bph4. From the genetic linkage map and QTL analysis of 95 and 78 F 2 individuals, the bph4 locus was mapped at the same chromosomal region of Bph3 between two flanking markers RM589 and RM586. Markers linked to the resistance gene explained 58.8-70.1% of the phenotypic variations and can be used for marker-assisted selection in BPH-resistant breeding programs. In addition, our experiment provides evidence that a recessive gene could behave as a dominant gene under different genetic backgrounds.

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Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2

Cited by 64 publications

References 21 publications

Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley

Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley

Efficient construction of high-density linkage map and its application to QTL analysis in barley

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

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