Construction of a high-resolution genetic map and identification of quantitative trait loci for salt tolerance in jute (Corchous spp.)

Abstract

Background Jute (Corchorus spp.) is the most important natural fiber crop after cotton in terms of cultivation area and production. Salt stress greatly restricts plant development and growth. A high-density genetic linkage map is the basis of quantitative trait locus (QTLs) mapping. Several high-density genetic maps and QTLs mapping related to salt tolerance have been developed through next-generation sequencing in many crop species. However, such studies are rare for jute. Only several low-density genetic maps have been constructed and no salt tolerance-related QTL has been mapped in jute to date. Results We developed a high-density genetic map with 4839 single nucleotide polymorphism markers spanning 1375.41 cM and an average distance of 0.28 cM between adjacent markers on seven linkage groups (LGs) using an F2 jute population, LGs ranged from LG2 with 299 markers spanning 113.66 cM to LG7 with 1542 markers spanning 350.18 cM. In addition, 99.57% of gaps between adjacent markers were less than 5 cM. Three obvious and 13 minor QTLs involved in salt tolerance were identified on four LGs explaining 0.58–19.61% of the phenotypic variance. The interval length of QTL mapping varied from 1.3 to 20.2 cM. The major QTL, qJST-1, was detected under two salt stress conditions that explained 11.81 and 19.61% of the phenotypic variation, respectively, and peaked at 19.3 cM on LG4. Conclusions We developed the first high-density and the most complete genetic map of jute to date using a genotyping-by-sequencing approach. The first QTL mapping related to salt tolerance was also carried out in jute. These results should provide useful resources for marker-assisted selection and transgenic breeding for salt tolerance at the germination stage in jute.

Jute Responses and Tolerance to Abiotic Stress: Mechanisms and Approaches

“…Yang et al [ 73 ] subsequently constructed a high-throughput quantitative trait locus (QTL) mapping, which consisted of 4839 markers on seven linkage groups (LG) that ranged from LG1–7 for improving salt-stress tolerance at the germination stage. Among these, three highly and thirteen slightly effective QTLs were found on four LGs under two salt stress treatments (140 and 160 mM NaCl), which were mainly implicated in salt tolerance.…”

Genetic analyses of morphological traits, and phenotypic screening of tossa jute germplasm grown under salinity stress

“…The yield of crops, adaptability & nutrient availability in soil, and activity of soil microorganisms depend on the electric conductivity (EC) of growing media, and which influence on the soil processes including greenhouse gases (NO 2 , CH 4 , and CO 2 ) emission. Salinity affects jute plants at any age [40] , and greatly hampers the plant growth and development [11] , [51] . Salt stress affects both water relation (osmotic stress) and ionic relations (ionic stress) leading to hampering the plant physiology [6] .…”