Construction of a GFP-BAR plasmid and its use for switchgrass transformation

Polyhydroxyalkanoate Polymers and Their Production in Transgenic Plants

“…These features, coupled with the positive environmental benefits associated with this crop and the ability to diversify U.S. agriculture, make this crop an attractive proposition for PHA production. Both particle bombardment (Richards et al, 2001) and Agrobacterium methods (B. V. Conger, unpublished results) have now been demonstrated to work.…”

Molecular Breeding of Grasses by Transgenic Approaches for Biofuel Production

“…Tungsten particles coated with a plasmid harboring the sgfp and bar genes were introduced into immature inflorescence-derived embryogenic calli. In total, 2,430 calli were subjected to the gene transfer, and 97 plants eventually showed tolerance, conferred by the bar gene, to 0.1% Basta herbicide (Richards et al, 2001). Basta tolerance was also observed in T 1 transgenic progeny resulting from crosses between transgenic and nontransgenic control plants, indicating inheritance of the bar gene (Richards et al, 2001).…”

“…The first transgenic switchgrass plants were produced by the gene gun method (Richards et al, 2001). Tungsten particles coated with a plasmid harboring the sgfp and bar genes were introduced into immature inflorescence-derived embryogenic calli.…”

Bioenergy Feedstock Development Program Status Report

“…In 1999, Richards et al 75 successfully transferred a gene that provides tolerance to a herbicide into an Alamo switchgrass genotype. At ORNL, various types of DNA markers are being used to investigate genetic identity among and variability within switchgrass genotypes.…”