Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.)

Abstract

A genetic linkage map with 70 loci (55 SSR, 12 AFLP and 3 morphological loci) was constructed using 117 F 2 plants obtained from a cross between two upland cotton cultivars Yumian 1 and T586, which have relatively high levels of DNA marker polymorphism and differ remarkably in fiber-related traits. The linkage map comprised of 20 linkage groups, covering 525 cM with an average distance of 7.5 cM between two markers, or approximately 11.8% of the recombination length of the cotton genome. The present genetic linkage map was used to identify and map the quantitative trait loci (QTLs) affecting lint percentage and fiber quality traits in 117 F 2:3 family lines. Sixteen QTLs for lint percentage and fiber quality traits were identified in six linkage groups by multiple interval mapping: four QTLs for lint percentage, two QTLs for fiber 2.5% span length, three QTLs for fiber length uniformity, three QTLs for fiber strength, two QTLs for fiber elongation and two QTLs for micronaire reading. The QTL controlling fiberrelated traits were mainly additive, and meanwhile including dominant and overdominant. Several QTLs affecting different fiber-related traits were detected within the same chromosome region, suggesting that genes controlling fiber traits may be linked or the result of pleiotropy.

Construction of a linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.)

“…Here we found many common characteristics of QTLs related to fiber quality traits as described in the previous reports involving interspecific maps [3,10,23,[33][34][35][36][37][38] and intraspecific maps [12,15,26,30,[39][40][41][42][43][44][45][46], although few common markers were used in the present research and the previous studies, and the maps covered different region parts of cotton genome, making it difficult to compare the common QTLs, some QTLs were detected and mapped on the same chromosomes and affect common traits. These include seven QTLs for fiber length located in the same chromosomal regions as reported earlier [10,26,34,41,42], two QTLs for fiber uniformity ratio located in the same chromosomal regions [43,46], two QTLs for fiber micronaire [10,43,44], three QTLs for fiber elongation [30,36,42,43,46,47], and 4 QTLs for fiber strength [26,34,36,43,44,46,47].…”

“…These include seven QTLs for fiber length located in the same chromosomal regions as reported earlier [10,26,34,41,42], two QTLs for fiber uniformity ratio located in the same chromosomal regions [43,46], two QTLs for fiber micronaire [10,43,44], three QTLs for fiber elongation [30,36,42,43,46,47], and 4 QTLs for fiber strength [26,34,36,43,44,46,47]. For example, the chromosome regions where QTLs were detected for fiber length in our research matched those in an interspecific map developed from an F 2 population [10]; the QTLs may be common QTLs for fiber quality traits.…”

Agronomic characterization, genetic diversity and association analysis of cotton cultivars using simple sequence repeat molecular markers

“…SSR molecular markers could also prove to be useful in the understanding of the molecular base of fiber growth, while they could be used in parallel in various breeding programs and mainly in programs that are aimed at improving the quality of cotton fiber as well as increasing yield. Recent studies have found that the molecular markers that show a correlation with the qualitative characteristics of fiber are located almost in all chromosomes of cotton (Guo et al, 2003;Park et al, 2005;Zhang et al, 2005;Abdurakhmonov et al, 2007;Kantartzi and Stewart, 2008). Molecular markers for lint percentage have been found on chromosomes 5, 9, 10, 12, 16, 18, 23, and 26, for micronaire on chromosomes 3, 9 and 16, for fiber strength on chromosomes 12, 15, 17, and 18, and for fiber length on chromosomes 2, 18 and 26.…”

A Microsatellite-Based, Gene-Rich Linkage Map Reveals Genome Structure, Function and Evolution in Gossypium

“…To 1 These authors contributed equally to this work. 2 date, several genetic maps of cotton genomes have been constructed using diverse DNA molecular markers and mapping populations (Ulloa and Meredith 2000;Ulloa et al 2002;Zhang et al 2002;Lacape et al 2003;Mei et al 2004;Rong et al 2004;Zhang et al 2005;Frelichowski et al 2006). The most saturated tetraploid cotton map is from Rong et al (2004), which is composed of 2584 loci at 1.72-cM intervals in 26 linkage groups.…”