Construction of a genetic linkage map of an interspecific diploid blueberry population and identification of QTL for chilling requirement and cold hardiness

Rowland, Lisa J.; Ogden, Elizabeth L.; Bassil, Nahla V.; Buck, Emily; McCallum, Susan; Graham, Julie; Brown, Allan; Wiedow, Claudia; Campbell, A. Malcolm; Haynes, Kathleen G.; Vinyard, Bryan T.

doi:10.1007/s11032-014-0161-9

Cited by 49 publications

(62 citation statements)

References 67 publications

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“…LGs was recently published for a population of interspecific blueberry hybrids of V. darrowii 9 V. corymbosum using a combination of 265 SNP, SSR, or RAPD markers (Rowland et al 2014). The linkage analysis in the CNJ02-1 population positioned 541 SSR loci on 12 LGs in the MQ 9 CQ map which spanned 1177.84 cM and covered 96 percent of the estimated 470 Mb cranberry genome.…”

Section: Discussionmentioning

confidence: 99%

“…Tetrad analysis of reciprocal translocation heterozygotes in two cranberry cultivars has previously revealed both genotypic and environmental effects on genetic recombination (Ortiz and Vorsa 1998). Because high levels of segregation distortion have been previously reported in mapping studies for cranberry and blueberry (Georgi et al 2013;Rowland et al 2014), extra precautions were taken to ensure correct marker order and to exclude distorted markers using conservative Chi-square tests (P \ 0.05). Strategies, such as reduced genome representation for developing SNPs, are being conducted to create a high-density SSR backbone/SNP linkage map that will be used to assess the occurrence of segregation distortion along the 12 cranberry LGs with a high degree of precision.…”

Section: Discussionmentioning

confidence: 99%

“…These studies were important sources of genetic resources for the first genetic map in cranberry, which included 33 SSRs derived from blueberry sequences (Georgi et al 2013), and in the current linkage map, which includes 19 of the 33 blueberry SSRs (Online Resource 1). However, only four of these blueberry-derived EST-SSRs have been included in a blueberry genetic map (Rowland et al 2014), and thus, no additional comparative inferences can be made at this time.…”

Section: Comparative Genomics In Vacciniummentioning

confidence: 99%

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Development of a high-density cranberry SSR linkage map for comparative genetic analysis and trait detection

et al. 2015

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Since its domestication 200 years ago, breeding of the American cranberry (Vaccinium macrocarpon) has relied on phenotypic selection because applicable resources for molecular improvement strategies such as marker-assisted selection (MAS) remain limited. To enable MAS in cranberry, the first high-density SSR linkage map with 541 markers representing all 12 cranberry chromosomes was constructed for the CNJ02-1 progeny from a cross of elite cultivars, CNJ97-105-4 and NJ98-23. The population was phenotyped for a 3-year period for total yield (TY), mean fruit weight (MFW), and biennial bearing index (BBI), and data were analyzed using mixed models and best linear unbiased predictors (BLUPs). Significant differences between genotypes were observed for all traits. Quantitative trait loci (QTL) analyses using BLUPs identified four MFW QTL on three linkage groups (LGs), three TY QTL on three LGs, and one BBI QTL which colocalized with a TY QTL. Local BLAST of a cranberry nuclear genome assembly identified homologous sequences for the mapped SSRs which were then anchored to 12 pseudo-chromosomes using the linkage map information. Analyses comparing coding regions (CDS) anchored in the cranberry linkage map with grape, kiwifruit, and tomato genomes were Electronic supplementary material The online version of this article (

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Comparative Genomics In Vacciniummentioning

confidence: 99%

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Development of a high-density cranberry SSR linkage map for comparative genetic analysis and trait detection

et al. 2015

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“…With the minimum repeat length of 12 base-pairs they are tandemly repeated usually 5-20 times in the genome (Goodfellow 1992;Vaughan and Lloyd 2003;Ellegren 2004;Prajapati et al 2017). As a result of their quickness, simplicity, rich polymorphism and stability SSR markers are highly popular in genetic diversity analysis (Turkoglu et al 2013;Gürcan et al 2015;Batnini et al 2016), construction of fingerprints (Cantini et al 2001;Rojas et al 2008;Klabunde et al 2014;Turet-Sayar et al 2012;Ivanovych et al 2017), genetic purity test (Spann et al 2010), molecular map construction and gene mapping (Ogundiwin et al 2009;Olukolu et al 2009;Fan et al 2010;Pacheco et al 2014;Rowland et al 2014;Wang et al 2014;Eduardo et al 2015), utilization of heterosis, especially in the identification of species that are genetically related. Microsatellite markers have also been used in several studies to define conserved regions among related species (Decroocq et al 2003;Martínez-Gómez et al 2003;Maghuly and Laimer 2011;Alisoltani et al 2016) for both plants and animals genome mapping (Weising et al1998).…”

Section: Introductionmentioning

confidence: 99%

Preliminary results of SSR based characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) genotypes cultivated in Hungary

Eren¹,

Bedő²,

Edosa³

et al. 2017

Columella

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Cherry cultivation in the Carpathian basin area began more than 100.000 years ago. Adapting to the basin specific ecological conditions resulted in high degree of genetic variability among the cherry cultivars. The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination their specific DNA fingerprints. Due to the high degree of polymorphism of microsatellite markers, generally only six SSR loci are enough to differentiate the varieties. Microsatellite markers are used not only for cultivar identification but also for the verification of synonyms and homonyms. Owing to their locus specificity and Mendelian codominant inheritance, parentage can be clearly identified, primary and secondary relationships between the cultivars can be discovered. The aim of this research was to characterize 29 sour cherry (Prunus cerasus L.), and 38 sweet cherry (Prunus avium L.) genotypes cultivated in Hungary to establish their DNA fingerprints in 6 SSR loci by allele numbers and sizes.

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“…이러한 ESTs 들은 블루베리와 블루베리속 식물들의 저온순화 연구에 서 도출되었으며, 블루베리에서는 꽃눈에서의 비순화와 저온순화에 대한 연구 (Dhanaraj et al 2004(Dhanaraj et al , 2006)와 진달 래속 식물에서는 비순화와 저온순화에 대한 잎눈에서의 발현 유전자 단편조합들이 구축되었다 (Wei et al 2005). (Bian et al 2014;Debnath 2014;Liu et al 2014;Rowland et al 2010;Rowland et al 2014 …”

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Current status and prospects of blueberry genomics research

Kim

Yun

2015

J Plant Biotechnol

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Blueberry (Vaccinium spp.) is a bush that grows well at special cultural environments such as acid soil, high organic matter content, and a good drainage and aeration compared to other general crops. Blueberries are well known to contain high amounts of anthocyanins and phenolic compounds, resulting in high antioxidant activity that provides health benefits, and expanding the cultivation areas and consumer's demand in the worldwide. However, the full genome of blueberry has not been announced until now. Furthermore, the genomic analysis and transcriptome approaches are not so popular compare to major crops such as orange, apple, and grape. The aim of the review about blueberry genomic research is to establish the platform for setting blueberry breeding target, increasing proficiency of blueberry research, and making the practical cultivation techniques in Korea. The main topics in the blueberry genomic research including transcriptome, genetic mapping, and various markers are related with cold hardiness, chilling requirement, hot tolerance, anthocyanin content, and flavonoid synthesis pathway on various tissues like flower bud, leaf bud, shoot, root, and berry fruit. The review of the current status of blueberry genomic research will provide basic information to the breeders and researchers and will contribute to development of blueberry industry with sustainable productions and increase of blueberry consumption as new profitable crops in Korea.

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Construction of a genetic linkage map of an interspecific diploid blueberry population and identification of QTL for chilling requirement and cold hardiness

Cited by 49 publications

References 67 publications

Development of a high-density cranberry SSR linkage map for comparative genetic analysis and trait detection

Development of a high-density cranberry SSR linkage map for comparative genetic analysis and trait detection

Preliminary results of SSR based characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) genotypes cultivated in Hungary

Current status and prospects of blueberry genomics research

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