Construction of a ganciclovir-sensitive lentiviral vector to assess the influence of angiopoietin-3 and soluble Tie2 on glioma growth

Beaudet, Marie-Josée; Rueda, Naika; Kobinger, Gary P.; Villeneuve, Jérôme; Vallières, Luc

doi:10.1007/s11060-009-0095-y

Cited by 7 publications

(8 citation statements)

References 31 publications

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“…1b, d, f, g and l). Leptomeningeal spread in human DIPG is often detected at recurrence [44] and it was found both in h-VUMC-DIPG-5 and in m-VU-DIPG-3 and -5 (Fig. 1b and Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Human pontine glioma cells can induce murine tumors

et al. 2014

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Diffuse intrinsic pontine glioma (DIPG), with a median survival of only nine months, is the leading cause of pediatric brain cancer mortality. Dearth of tumor tissue for research has limited progress in this disease until recently. New experimental models for DIPG research are now emerging. To develop preclinical models of DIPG, two different methods were adopted: cells obtained at autopsy 1) were directly xenografted orthotopically into the pons of immunodeficient mice without an intervening cell culture step or 2) were first cultured in vitro and, upon successful expansion, injected in vivo. Both strategies resulted in pontine tumors histopathologically similar to the original human DIPG tumors. However, following the direct transplantation method all tumors proved to be composed of murine and not of human cells. This is in contrast to the indirect method that included initial in vitro culture and resulted in xenografts comprised of human cells. Of note, direct injection of cells obtained post mortem from the pons and frontal lobe of human brains not affected by cancer did not give rise to neoplasms. The murine pontine tumors exhibited an immunophenotype similar to human DIPG, but were also positive for microglia/macrophage markers, such as CD45, CD68 and CD11b. Serial orthotopic injection of these murine cells results in lethal tumors in recipient mice. Direct injection of human DIPG cells in vivo can give rise to malignant murine tumors. This represents an important caveat for xenotransplantation models of DIPG. In contrast, an initial in vitro culture step can allow establishment of human orthotopic xenografts. The mechanism underlying this phenomenon observed with direct xenotransplantation remains an open question.

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Section: Resultsmentioning

confidence: 99%

Human pontine glioma cells can induce murine tumors

et al. 2014

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“…The glioblastoma cells often exploits the existing host vessels to grow or invade, which is vessel cooption [16,17]. Vessel cooption was a strong explanation for the invasion or metastasis of malignant brain tumors [16][17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

“…Vessel cooption was a strong explanation for the invasion or metastasis of malignant brain tumors [16][17][18][19][20]. The coopted vasculature initiates structural and functional change to better suit glioma growth [21].…”

Section: Discussionmentioning

confidence: 99%

“…ANGPTLs family participates in the regulation of angiogenesis but there are few reports concerning the relationship between the ANGPTLs and glioma. ANGPTL1 was proven to increase capillary destabilization and promote glioma growth and invasiveness by vessel cooption [16]. ANGPTL4 was increased by epidermal growth factor variant type III (EGFRvIII) and involved in the angiogenesis and glioma growth [27].…”

Section: Discussionmentioning

confidence: 99%

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Clinical significance of angiopoietin-like protein 3 expression in patients with glioblastoma

Wang¹,

Li²,

Qi³

et al. 2016

neo

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There is strong evidence that angiopoietin-like peptide family is involved in the invasiveness and metastasis of cancer. Angiopoietin-like protein 3 (ANGPTL3) is proven to be involved in angiogenesis and tumor development. However, there is no published data on the role ANGPLT3 plays in glioblastomas. The present study was conducted to examine ANGPLT3 proteins expression and its association with clinicopathological factors and prognosis in human glioblastomas. Immunohistochemistry was used to evaluate the expression of ANGPTL3, EGFR and VEGFR. We found that 33 cases (57.9%) that showed strong immunostaining for ANGPTL3 proteins. However, there was no significant difference between the expression of ANGPTL3 and the proangiogenic factors, including EGFR or VEGFR. Patients with high/moderate expression of ANGPTL3 had a significantly shorter survival time (6.3 months) than those (median survival time 13.8 months) with low/ negative expression. The overall survival (OS) was also investigated and analyzed by the Kaplan-Meier method, which showed a significant difference (P=0.0045, Breslow test). The present data leads to new insights into the role of ANGPTL3 in glioblastomas and provides an independent predictive factor.

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“…However, overexpression of Ang-3 might inhibit tumor angiogenesis in pulmonary metastasis of Lewis lung carcinoma and TA3 mammary carcinoma cells (Xu et al, 2004). Further studies found that Ang-3 was expressed in the uterus, placent, and testes (Kim et al, '99;Matsumoto et al, 2002;Beaudet et al, 2010). However, Ang-3 expression and hormonal regulation in mouse uterus during the peri-implantation were still not defined in detail so far.…”

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confidence: 99%

Differential Expression and Regulation of Angiopoietin‐3 in Mouse Uterus during Preimplantation Period

Guo

Zhang

et al. 2012

J Exp Zool Pt B

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Angiogenesis is necessary for successful implantation and decidualization. This study was to investigate the differential expression of angiopoietin-3 (Ang-3) in mouse uterus during early pregnancy and its regulation by steroid hormones using in situ hybridization and reverse transcription polymerase chain reaction (RT-PCR). There was no detectable Ang-3 mRNA signal on days 1-5 of pregnancy by in situ hybridization. On day 6 of pregnancy, a low level of Ang-3 mRNA signal was seen in the primary decidua. Ang-3 mRNA expression gradually increased on days 7 and 8 of pregnancy along with the development of decidua, and its expression scope was also expanded. The RT-PCR result indicated that Ang-3 mRNA expression was low on days 1-4 of pregnancy. On day 5, as embryo implanted, Ang-3 mRNA was highly expressed in mouse uterus, and the expression gradually increased on days 6-8 of pregnancy, with peak level on day 8 of pregnancy. Similarly, Ang-3 mRNA was also strongly expressed in decidualized cells under artificial decidualization. Compared with the delayed uterus, a high level of Ang-3 mRNA expression was detected in activated implantation uterus by RT-PCR. In the ovariectomized mouse uterus, Ang-3 mRNA expression increased and reached the highest level at 12 hr after injection of estrogen, progesterone, and estrogen plus progesterone, respectively. These results suggest that Ang-3 may play an important role during the process of mouse decidualization. Both estrogen and progesterone can induce the expression of Ang-3 in ovariectomized mouse uterus.

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Construction of a ganciclovir-sensitive lentiviral vector to assess the influence of angiopoietin-3 and soluble Tie2 on glioma growth

Cited by 7 publications

References 31 publications

Human pontine glioma cells can induce murine tumors

Human pontine glioma cells can induce murine tumors

Clinical significance of angiopoietin-like protein 3 expression in patients with glioblastoma

Differential Expression and Regulation of Angiopoietin‐3 in Mouse Uterus during Preimplantation Period

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