Construction of a Dual-Mode Immune Platform Based on the Photothermal Effect of AgCo@NC NPs for the Detection of α-Fetoprotein

Abstract

Compared with the accuracy of a single signal and the limitation of environmental applicability, the application value of dual-mode detection is gradually increasing. To this end, based on the photothermal effect of Ag/Co embedded N-rich mesoporous carbon nanomaterials (AgCo@NC NPs), we designed a dual-mode signal response system for the detection of α-fetoprotein (AFP). First, AgCo@NC NPs act as a photothermal immunoprobe that converts light energy into heat driven by a near-infrared (NIR) laser and obtains temperature changes corresponding to the analyte concentration on a hand-held thermal imager. In addition, this temperature recognition system can significantly improve the efficiency of Fenton-like reactions. AgCo@NC NPs act as peroxidase mimics to initiate the generation of poly N-isopropylacrylamide (PNIPAM, resistance enhancer) by cascade catalysis and the degradation of methylene blue (MB), thus enabling electrochemical testing. The dual-mode assay ranges from 0.01 to 100 and 0.001−10 ng/mL, with lower limits of detection (LOD) of 3.2 and 0.089 pg/mL, respectively, and combines visualization, portability, and high efficiency, opening new avenues for future clinical diagnostics and inhibitor studies.

Pt/Zn-TCPP Nanozyme-Based Flexible Immunoassay for Dual-Mode Pressure–Temperature Monitoring of Low-Abundance Proteins

“…The gas-producing reaction of H 2 O 2 decomposition in a microdetection cell was selected as the source of pressure. For improving the efficiency of signal recognition, Pt/Zn-TCPP was chosen as a peroxidase mimic to enhance gas generation . To boost the loading of Pt NPs, we utilized Zn-TCPP sheets, which appeared as flakes measuring about 100 nm in size (Figure S3A).…”