Construction of a “Battlements” Structure by Zirconium Glyphosate on Silica Nanospheres and the Catalytic Stability Promotion of Candida lipolytica Lipase on the Hybrid Materials

Abstract

Different diameter SiO 2 coated with zirconium glyphosate (ZrGP) was prepared, and Candida lipolytica lipase was loaded on the surface of ZrGP@SiO 2 in this study. ZrGP was in situ synthesized on the surface of SiO 2 and existed like "battlements". Under protection of the battlements structure and organic parts in ZrGP, the immobilization efficiency and activity recovery of Candida lipolytica lipase were improved greatly: 69.0% of the enzyme immobilization efficiency and 71.2% of the activity recovery were found after the lipase was loaded under certain immobilization conditions and the optimum catalytic environment. Besides, the immobilized enzyme was found to have better thermostability (up to 43 °C), wider pH value adaptation (from 6.9 to 8.4), and high reserved activity (over 80% remained after four cycles of catalysis).

d-Amino Acid Oxidase Immobilized on Pt Nanoparticle-Loaded Porous SiO2 Nanospheres Coated with a Zirconium-Based Coordination Polymer for Catalytic Deamination of d-Alanine

“…1 Silica (SiO 2 )-based materials have been extensively investigated for immobilization of enzymes. 2,3 The enzyme immobilizations can be categorized into three types, encapsulation using SiO 2 capsules, 4,5 core SiO 2 nanoparticles coated with functional materials for enzyme immobilization, 6−8 and enzymes embedded in nano-and microporous SiO 2 . 9,10 Using metal−organic framework ZIF-8 nanoparticles as templates, glucose oxidase and horseradish peroxidase were coencapsulated by SiO 2 capsules.…”

“…Enzyme immobilization on solid supports allows for enzyme reusability and improves enzyme stability, substrate specificity, and reactivity . Silica (SiO 2 )-based materials have been extensively investigated for immobilization of enzymes. , The enzyme immobilizations can be categorized into three types, encapsulation using SiO 2 capsules, , core SiO 2 nanoparticles coated with functional materials for enzyme immobilization, − and enzymes embedded in nano- and microporous SiO 2 . , Using metal–organic framework ZIF-8 nanoparticles as templates, glucose oxidase and horseradish peroxidase were coencapsulated by SiO 2 capsules . Fe 3 O 4 nanoparticles coated with SiO 2 were used as templates for preparing hollow porous nanocomposites to immobilize lipase .…”