Construction of 3D printed constructs based on microfluidic microgel for bone regeneration

Chai, Ningwen; Zhang, Jingtian; Zhang, Qianqian; Du, Haibo; He, Xi; Jin, Yang; Zhou, Xiaojun; He, Jiangjiang; He, Chuanglong

doi:10.1016/j.compositesb.2021.109100

Cited by 53 publications

(43 citation statements)

References 39 publications

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“…Scaffolds for bone regeneration [102] Schiff base reaction with diamine in w/o inverse emulsion magnetic NPs and DOX DOX release [103] 4.1. Self-Assembled Polysaccharide NGs…”

Section: Bone Marrow Mesenchymal Stem Cellsmentioning

confidence: 99%

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Current Advances of Polysaccharide-Based Nanogels and Microgels in Food and Biomedical Sciences

Papagiannopoulos

Sotiropoulos²

2022

Polymers

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Polysaccharides are natural polymers with hydrophilic, biocompatible and biodegradable characteristics and have many opportunities in the food and pharmaceutical sectors. This review focuses on the field of nano and microstructures whose internal structure is based on networked polysaccharide chains in 3D i.e., polysaccharide nanogels (NGs) and microgels (MGs). As it is observed the number of articles on NGs and MGs in peer reviewed scientific journals has been increasing over the last two decades. At the same time, the relative contribution of polysaccharides in this field is gaining place. This review focuses on the different applied methods for the fabrication of a variety of polysaccharide-based NGs and MGs and aims to highlight the recent advances on the subject and present their potentials and properties with regards to their integration in aspects of medicinal and food sciences. The presentation of the recent advances in the application of polysaccharide NGs and MGs is divided in materials with potential as emulsion stabilizers and materials with potential as carriers of bioactives. For applications in the medical sector the division is based on the fabrication processes and includes self-assembled, electrostatically complexed/ionically crosslinked and chemically crosslinked NGs and MGs. It is concluded that many advances are expected in the application of these polysaccharide-based materials and in particular as nutrient-loaded emulsion stabilizers, viscosity modifiers and co-assembled structures in combination with proteins.

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“…Scaffolds for bone regeneration [102] Schiff base reaction with diamine in w/o inverse emulsion magnetic NPs and DOX DOX release [103] 4.1. Self-Assembled Polysaccharide NGs…”

Section: Bone Marrow Mesenchymal Stem Cellsmentioning

confidence: 99%

“…This resulted in similar bone regeneration efficiencies. Microfluidic technology has been also used for core-shell cell-laden microparticles with a CaCl 2 -crosslinked Alg cell and a collagen core [ 103 ]. The MGs could increase the cell viability under the deposition by a 3D-bioprinter.…”

Section: Medical Applications Of Polysaccharide Ngs and Mgsmentioning

confidence: 99%

Current Advances of Polysaccharide-Based Nanogels and Microgels in Food and Biomedical Sciences

Papagiannopoulos

Sotiropoulos²

2022

Polymers

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“…[ 189 ] Bio‐inks from GelMA microgels can be further printed or assembled into larger‐scale structures. [ 190–192 ] Combined use of gelatin and GelMA is promising for the development of microgel‐based materials to form constructs with voids, as gelatin can be sacrificed while GelMA are more thermostable. [ 193 ]…”

Section: Diversity In Materials For Microgelsmentioning

confidence: 99%

Recent Advances in Microgels: From Biomolecules to Functionality

Zhu

Denduluri

et al. 2022

Small

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The emerging applications of hydrogel materials at different length scales, in areas ranging from sustainability to health, have driven the progress in the design and manufacturing of microgels. Microgels can provide miniaturized, monodisperse, and regulatable compartments, which can be spatially separated or interconnected. These microscopic materials provide novel opportunities for generating biomimetic cell culture environments and are thus key to the advances of modern biomedical research. The evolution of the physical and chemical properties has, furthermore, highlighted the potentials of microgels in the context of materials science and bioengineering. This review describes the recent research progress in the fabrication, characterization, and applications of microgels generated from biomolecular building blocks. A key enabling technology allowing the tailoring of the properties of microgels is their synthesis through microfluidic technologies, and this paper highlights recent advances in these areas and their impact on expanding the physicochemical parameter space accessible using microgels. This review finally discusses the emerging roles that microgels play in liquid–liquid phase separation, micromechanics, biosensors, and regenerative medicine.

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“…[ 24 ] Unlike conventional tissue engineering methods, 3D bioprinting is able to co‐printing a wide range of living cells and biomaterials and builds personalized macro/microstructures as needed. [ 25,26 ] However, nifedipine is difficult to fully exert its function due to the rapid release of Eth in the hydrogel scaffolds. [ 27 ] Laponite (Lap) is a 1–2 nm‐thick disk‐shaped 2D nanoclay, which exhibits dual charge distribution with permanent negative charge on the surface of the particle and positive charge along the edges.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Sympathetic Activation by Delivering Calcium Channel Blockers from a 3D Printed Scaffold to Promote Bone Defect Repair

Jin

et al. 2022

Adv Healthcare Materials

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Enhancing osteogenesis by promoting neural network reconstruction and neuropeptide release is considered to be an attractive strategy for repairing of critical size bone defects. However, traumatic bone defects often activate the damaged sympathetic nervous system (SNS) in the defect area and release excessive catecholamine to hinder bone defect repair. Herein, a 3D printed scaffold loaded with the calcium channel blocker‐nifedipine is proposed to reduce the concentration of catecholamine present in the bone defect region and to accelerate bone healing. To this end, nifedipine‐loaded ethosome and laponite are added into a mixed solution containing sodium alginate, methacrylated gelatin, and bone mesenchymal stem cells (BMSCs) to prepare a cell‐laden scaffold using 3D bioprinting. The released nifedipine is able to close the calcium channels of nerve cells, thereby blocking sympathetic activation and ultimately inhibiting the release of catecholamine by sympathetic nerve cells, which further promotes the osteogenic differentiation and migration of BMSCs, inhibits osteoclastogenesis in vitro, and effectively improves bone regeneration in a rat critical‐size calvarial defect model. Therefore, the results suggest that sustained release of nifedipine from the scaffold can effectively block SNS activation, providing promising strategies for future treatment of bone defects.

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Construction of 3D printed constructs based on microfluidic microgel for bone regeneration

Cited by 53 publications

References 39 publications

Current Advances of Polysaccharide-Based Nanogels and Microgels in Food and Biomedical Sciences

Current Advances of Polysaccharide-Based Nanogels and Microgels in Food and Biomedical Sciences

Recent Advances in Microgels: From Biomolecules to Functionality

Inhibition of Sympathetic Activation by Delivering Calcium Channel Blockers from a 3D Printed Scaffold to Promote Bone Defect Repair

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