Construction and validation of a competing endogenous RNA network in the thymus of miR-147−/− mice

Song, Gui-yuan; Hu, Yongjian; Zhang, Nan; Wang, Fei; Chao, Tianzhu; Wu, Yaoyao; Li, Yufeng; Xu, Ping

doi:10.1016/j.intimp.2023.109896

Cited by 1 publication

(2 citation statements)

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“…C57BL/6N and miR-147 −/− mice (4 weeks old) were acquired from Beijing Wei-tong-li-Hua Experimental Animal Technology Co. And the Chinese-French Immunoregulatory Genes Laboratory of Xinxiang Medical University, respectively. The deletion of miR-147 in these mice and associated genotypic changes were reported previously ( Song et al, 2023 ). Mice were individually housed under controlled conditions (22°C, 55% humidity, 12 h light/dark cycle).…”

Section: Materials Methodssupporting

confidence: 76%

“…In a previous study, our group determined that knocking down miR-147 was associated with significant changes in thymic lncRNA, miRNA, and mRNA expression in mice. In radiation-protected mouse lungs, the ability of troxerutin to upregulate PDPK1 was found to be mediated through miR-147 targeting and the consequent activation of AKT together with the inhibition of JNK activity ( Song et al, 2023 ). To better clarify the role that knocking out miR-147 has on the murine lungs, lung tissues from miR-147 −/− mice were thus used for sequencing.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the lncRNA-miRNA-mRNA ceRNA network in lungs of miR-147 −/− mice

Zhang,

Song,

et al. 2024

Front. Pharmacol.

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Background: Previous studies have documented important roles for microRNA-147 (miR-147) in inflammation, radiation-induced injury, cancer, and a range of other diseases. Murine lungs exhibit high levels of miRNA, mRNA, and lncRNA expression. However, very little research to date has focused on the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) networks associated with miR-147, and the regulation of lncRNAs and miRNAs in this setting remains poorly understood.Methods: After establishing a miR-147−/− model mouse, samples of lung tissue were harvested for RNA-sequencing, and differentially expressed lncRNAs, miRNAs, and mRNAs were identified. The miRNA targets of these lncRNAs and the identified miRNAs were first overlapped to facilitate the prediction of target mRNAs, with analyses then examining the overlap between these targets and mRNAs that were differentially expressed. Then, these target mRNAs were subjected to pathway enrichment analyses. These results were ultimately used to establish a miR-147-related ceRNA network.Results: Relative to wild-type mice, the lungs of miR-147−/− mice exhibited 91, 43, and 71 significantly upregulated lncRNAs, miRNAs, and mRNAs, respectively, together with 114, 31, and 156 that were significantly downregulated. The lncRNA-miRNA-mRNA network established based on these results led to the identification of Kcnh6 as a differentially expressed hub gene candidate and enabled the identification of a range of regulatory relationships. KEGG pathway enrichment showed that the mRNA targets of differentially expressed lncRNAs and miRNAs in the mice were associated with tumor-related signaling, endometrial cancer, bladder cancer, and ErbB signaling.Conclusion: These results suggest that the identified ceRNA network in miR-147−/− mice shapes tumor-associated signaling activity, with miR-147 potentially regulating various lncRNAs and miRNAs through Kcnh6, ultimately influencing tumorigenesis. Future studies of the lncRNA, miRNA, and mRNA regulatory targets shown to be associated with miR-147 in the present study may ultimately lead to the identification of novel clinically relevant targets through which miR-147 shapes the pathogenesis of cancer and other diseases.

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Section: Materials Methodssupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%