Construction and validation of a novel signature based on epithelial-mesenchymal transition–related genes to predict prognosis and immunotherapy response in hepatocellular carcinoma by comprehensive analysis of the tumor microenvironment

Gao, Biao; Wang, Yafei; Lu, Shichun

doi:10.1007/s10142-022-00933-w

Cited by 11 publications

(1 citation statement)

References 38 publications

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“…1 d). Since EMT (epithelial to mesenchymal transition) is closely related to CRC metastasis (Wei et al 2019 ), and EMT markers might indicate early occurrence of cancer metastasis (Zhang et al 2021 ), it is also addressed that EMT-related associated genes might predict the prognosis and the response to immunotherapy (Gao et al 2022 ). We also checked the EMT markers in tumour tissues from non-metastatic and metastatic patients by RT-PCR assay.…”

Section: Resultsmentioning

confidence: 99%

CLASRP oncogene as a novel target for colorectal cancer

Gu,

Wu,

et al. 2023

Funct Integr Genomics

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Clk4-associated serine/arginine-rich protein (CLASRP), an alternative splicing regulator, may be involved in the development and progression of cancer by regulating the activity of the CDC-like kinase (Clk) family. This study explored the biological function of CLASRP in colorectal cancer (CRC). The expression of CLASRP, which is associated with clinicopathological features, was analysed in CRC tissues and paired noncancer tissues by RT–PCR. The roles of CLASRP were investigated in CRC cells transfected with plasmids or shRNA through proliferation, migration and invasion assays in vitro and a xenograft model in vivo. Apoptosis was analysed using CLASRP-overexpressing CRC cells by western blotting. Clk inhibitors were used to perform functional research on CLASRP in CLASRP-overexpressing CRC cells. CLASRP was significantly upregulated in CRC cell lines, while high CLASRP expression was correlated with metastasis in CRC patients. Functionally, overexpression of CLASRP significantly promoted the proliferation, migration and invasion of CRC cells in vitro and tumour growth in vivo. Mechanistically, the proliferation, migration and invasion of CLASRP-overexpressing CRC cells were inhibited by Clk inhibitors, accompanied by low expression of CLASRP at the gene and protein levels. Clk inhibitors induced apoptosis of CLASRP-overexpressing CRC cells, resulting in direct blockade of cell growth. The expression levels of cleaved caspase 3 and cleaved caspase 8 were increased in CLASRP-overexpressing CRC cells treated with Clk inhibitors. CLASRP might serve as a promotional oncogene in CRC cells and be suppressed by Clk inhibitors through activation of caspase pathways.

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Section: Resultsmentioning

confidence: 99%

CLASRP oncogene as a novel target for colorectal cancer

Gu,

Wu,

et al. 2023

Funct Integr Genomics

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RETRACTED ARTICLE: RAD54L promotes progression of hepatocellular carcinoma via the homologous recombination repair pathway

Zhuang

Gu³

et al. 2023

Funct Integr Genomics

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RETRACTED ARTICLE: Circular RNA circDLG1 contributes to HCC progression by regulating the miR-141-3p/WTAP axis

Wang,

Yu,

Wang

et al. 2023

Funct Integr Genomics

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This study aims to explore novel and reliable biomarkers for predicting hepatocellular carcinoma (HCC) prognosis. Circular RNAs (circRNAs) were determined by analysis of human circRNA arrays and quantitative reverse transcription polymerase reactions. To test for an interaction between circDLG1, we used luciferase reporter assays, RNA immunoprecipitation, and fluorescence in situ hybridization assays that were employed to test the interaction between circDLG1, miR-141-3p, and WTAP. q-RT-PCR and western blot were used to evaluate the target regulation of miR-141-3p and WTAP. shRNA-mediated knockdown of circDLG1, proliferation, migration, and invasion experiment of metastasis were used to evaluate the function of circDLG. CircDLG1 rather than lining DLG1 was upregulated in HCC tissues, from HCC patients as well as HCC cell lines compared to normal controls. circDLG1 high expression in HCC patients was correlated with shorter overall survival. Knockdown of circDLG1 and miR-141-3p mimic could inhibit the tumorigenesis of HCC cells in vivo and in vitro. Importantly, we demonstrated that circDLG1 could act as a sponge of miR-141-3p to regulate the expression of WTAP, and further suppress the tumorigenesis of HCC cells. Our study reveals that circDLG1 can serve as a novel potential circulating biomarker for the detection of HCC. circDLG1 participates in the progression of HCC cells by sponging miR-141-3p with WTAP, providing new insight into the treatment of HCC.

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Construction and validation of a novel signature based on epithelial-mesenchymal transition–related genes to predict prognosis and immunotherapy response in hepatocellular carcinoma by comprehensive analysis of the tumor microenvironment

Cited by 11 publications

References 38 publications

CLASRP oncogene as a novel target for colorectal cancer

CLASRP oncogene as a novel target for colorectal cancer

RETRACTED ARTICLE: RAD54L promotes progression of hepatocellular carcinoma via the homologous recombination repair pathway

RETRACTED ARTICLE: Circular RNA circDLG1 contributes to HCC progression by regulating the miR-141-3p/WTAP axis

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