Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseriaï¿½meningitidis serogroup B

Wu, Xiaoxia; Li, Kaiming; Xie, Meihua; Yu, Min; Tang, Shuangyang; Li, Zhenyu; Hu, Sihai

doi:10.3892/mmr.2017.8255

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…For example, cells of Yersinia pestis that are devoid of NlpD were chained, as well as displayed heat sensitivity and an inability to disseminate into the mice organs ( Tidhar et al, 2009 , 2019 ). Due to the highly attenuated phenotype, Y. pestis Δ nlpD was further explored as a promising vaccine candidate against plague ( Wu et al, 2018 ).…”

Section: Physiological Functions Of M23 Peptidasesmentioning

confidence: 99%

One fold, many functions—M23 family of peptidoglycan hydrolases

Razew¹,

Schwarz²,

Mitkowski³

et al. 2022

Front. Microbiol.

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Bacterial cell walls are the guards of cell integrity. They are composed of peptidoglycan that provides rigidity to sustain internal turgor and ensures isolation from the external environment. In addition, they harbor the enzymatic machinery to secure cell wall modulations needed throughout the bacterial lifespan. The main players in this process are peptidoglycan hydrolases, a large group of enzymes with diverse specificities and different mechanisms of action. They are commonly, but not exclusively, found in prokaryotes. Although in most cases, these enzymes share the same molecular function, namely peptidoglycan hydrolysis, they are leveraged to perform a variety of physiological roles. A well-investigated family of peptidoglycan hydrolases is M23 peptidases, which display a very conserved fold, but their spectrum of lytic action is broad and includes both Gram- positive and Gram- negative bacteria. In this review, we summarize the structural, biochemical, and functional studies concerning the M23 family of peptidases based on literature and complement this knowledge by performing large-scale analyses of available protein sequences. This review has led us to gain new insight into the role of surface charge in the activity of this group of enzymes. We present relevant conclusions drawn from the analysis of available structures and indicate the main structural features that play a crucial role in specificity determination and mechanisms of latency. Our work systematizes the knowledge of the M23 family enzymes in the context of their unique antimicrobial potential against drug-resistant pathogens and presents possibilities to modulate and engineer their features to develop perfect antibacterial weapons.

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Section: Physiological Functions Of M23 Peptidasesmentioning

confidence: 99%

One fold, many functions—M23 family of peptidoglycan hydrolases

Razew¹,

Schwarz²,

Mitkowski³

et al. 2022

Front. Microbiol.

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“…Concurrently, using a classical biochemical fractionation approach, fHbp was identified as a key vaccine antigen and is now a component of the licensed meningococcal vaccine Trumeba Next generation vaccines for improved protection against serogroup B disease have also been developed. These include chimeric vaccine antigens, which are composed of an fHbp scaffold onto which an immunogenic PorA moiety is grafted 105 ; OMVs with over-expressed fHbp proteins 106 ; a subunit opa vaccine, composed of 14 different opa variants 107 and an NMB0315 DNA vaccine, which expresses the outer-membrane protein NMB0315 inside host cells 108 .…”

Section: Meningococcal Vaccinesmentioning

confidence: 99%

An Overview of Neisseria meningitidis

Hollingshead

Tang

2019

Methods in Molecular Biology

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Methods in Molecular Biology Introductory Overview Overview of Neisseria meningitidisNeisseria meningitidis, or the meningococcus, is a Gram negative, non-motile diplococcus that can cause septicaemia and meningitis in susceptible individuals. It is closely related to N. gonorrhoeae, or the gonococcus, which is the causative agent of the sexually transmitted infection gonorrhoea. N. meningitidis and N. gonorrhoeae are the only pathogenic members of the genus Neisseria, which includes several commensal species 1 . Both N. meningitdis and N. gonorrhoeae are obligate human pathogens. N. meningitidis is acquired through person-to-person contact via aerosols and oral or nasal secretions and is a member of the normal nasopharyngeal microbiome in healthy individuals. Once acquired, the meningococcus may be carried transiently or for a period of several months before carriage is lost. Carriage studies have shown N. meningitidis resides in 3-35% of the population depending on geographic location, climate and local disease status 2 . N. meningitidis is considered an accidental pathogen, as the bacterium only rarely crosses into the bloodstream causing life-threatening diseases such as septicaemia and meningitis. Meningococcal disease occurs endemically as sporadic cases in a community, or in epidemics, such as those observed in the African meningitis belt. The most susceptible individuals are infants under 1 year of age, teenagers and young adults 3,4 . Others at risk from meningoccal disease are those with deficiencies in their complement system and asplenia 4 . The onset of disease is rapid and case fatality rates range between 10-20%, despite the availability of antibiotic treatment. Of the survivors, 10-20% develop long-term sequelae, including hearing loss, loss of limbs, skin scarring and neurodevelopmental deficits, and up to 36% develop deficits in physical, cognitive or psychological functioning 5, 6 .

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Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseriaï¿½meningitidis serogroup B

Cited by 2 publications

References 26 publications

One fold, many functions—M23 family of peptidoglycan hydrolases

One fold, many functions—M23 family of peptidoglycan hydrolases

An Overview of Neisseria meningitidis

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