Construction and intergeneric conjugative transfer of a pSG5-based cosmid vector from<i>Escherichia coli</i>to the polyisoprene rubber degrading strain<i>Micromonospora aurantiaca</i>W2b

Rose, Karsten; Steinbüchel, Alexander

doi:10.1111/j.1574-6968.2002.tb11214.x

Cited by 16 publications

(4 citation statements)

References 19 publications

(20 reference statements)

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“…Members of the second group show weaker growth in submers culture but grow and form clear zones on NR latex-overlay agar plates. These bacteria belong to streptomycetes and related genera. ,, …”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Genes fromStreptomycessp. Strain K30 Responsible for Clear Zone Formation on Natural Rubber Latex and Poly(cis-1,4-isoprene) Rubber Degradation

2004

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Streptomyces sp. strain K30 was isolated from soil next to a city high way in Münster (Germany) according to its ability to degrade natural and synthetic poly(cis-1,4-isoprene) rubber and to form clear zones on natural rubber latex agar plates. The clear zone forming phenotype was used to clone the responsible gene by phenotypic complementation of a clear zone negative mutant. An open reading frame (lcp) of 1,191 bp was identified, which was preceded by a putative signal sequence and restored the capability to form clear zones on natural rubber latex in the mutant. The putative translation product exhibited strong homologies (50% aa identity) to a putative secreted protein from Streptomyces coelicolor strain A3(2), another clear zone forming strain. Heterologous expression of lcp of Streptomyces sp. strain K30 in Streptomyces lividans strain TK23 enabled the latter to form clear zones on latex-overlay agar plates and to accumulate a degradation product of about 12 kDa containing aldehyde groups. Two ORFs putatively encoding a heterodimeric molybdenum hydroxylase (oxiAB) were identified downstream of lcp in Streptomyces sp. strain K30 strain which exerted a positive effect on clear zone formation and enabled the strain to oxidize the resulting aldehydes. Heterologous expression of a fragment harboring lcp plus oxiAB in S. lividans TK23 resulted in accumulation of aldehydes only in the presence of 10 mM tungstate. Determination of protein content during cultivation on poly(cis-1,4-isoprene) revealed an increase of the cellular protein, and gel permeation chromatography analysis indicated a shift of the molecular weight distribution of the rubber to lower values in the transgenic S. lividans strains and in the wild type, thus confirming utilization and degradation of rubber. Therefore, for the first time, genes responsible for clear zone formation on natural rubber latex and synthetic cis-1,4-polyisoprene degradation in Gram-positive bacteria were identified and characterized.

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Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Genes fromStreptomycessp. Strain K30 Responsible for Clear Zone Formation on Natural Rubber Latex and Poly(cis-1,4-isoprene) Rubber Degradation

2004

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“…The devulcanization process of rubber involves the breaking of the multiple C-S bonds along with the polymeric chains, where removing sulfur makes the recycling of rubber easier. Aerobic and anaerobic microorganisms are being intensively studied as devulcanization prompters, where the metabolic pathways and the enzymes involved, are becoming better known 24–26 . The presence of actinomycetes to metabolize purified natural rubber as the only source of carbon has already been described since 1995, when Heisey and Papadatos, based on fatty acid profiles and cell walls showing seven strains of Streptomyces , two of Amycolatopsis and one of Nocardia , the authors further concluded that actinomycetes were the major organisms involved in the biodegradation of natural rubber hydrocarbons 27 .…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Vulcanized SBR: A Comparison between Bacillus subtilis, Pseudomonas aeruginosa and Streptomyces sp

Aboelkheir

Bedor

Leite

et al. 2019

Sci Rep

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Rubber residues present harmful impacts on health and environment, besides wasting valuable and huge amounts of rubber. Biological recycling technique is focused here to minimize this problem. A comparison of the biodegradation effect caused by Bacillus subtilis, Pseudomonas aeruginosa, and Streptomyces sp., separately, on vulcanized SBR-rubber during 4 weeks is reported. The surface and molecular analyses were studied by FTIR-ATR, TGA, DSC, TC and SEM/EDS, in addition to the contact angle and crosslinking tests. B. subtilis, P. aeruginosa, and Streptomyces sp. evoked after 4 weeks a loss in v-SBR crosslinks by 17.15, 10.68 and 43.39% and also in the contact angle with water by 14.10, 12.86 and 15.71%, respectively., if compared to Control samples. FTIR findings indicate that the polymeric chain has been partially consumed causing C-C bonds scission indicating the biodegradation and bio-devulcanization phenomena. The bacterial strains caused a carbon loss by 9.15, 5.97 and 4.55% after one week and 16.09, 16.79 and 18.13% after four weeks for B. subtilis, P. aeruginosa, and Streptomyces sp. mediums, respectively. DSC and EDS results are also promising and highlighting Streptomyces sp. strain as the most effective biodegradative one as an alternative and natural mean of degrading vulcanized rubber residues.

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“…These bacteria belong to Streptomycetes and related genera [46,47] . From the several microorganisms isolated and tested Actinomycetes showed the ability to degrade NR and to use it as carbon source [48] . Both Nocardia strain [34] and Xanthomonas strain [35] led to accumulation of oligomers with molar mass around 100 g/mol.…”

Section: Biodegradationmentioning

confidence: 99%

“…Numerous publications, as for example [21,[25][26][27][28][29][30][31][32][33][34][35][41][42][43][44][45][46][47][48][49][50][51][52][53][54] , are devoted to oxidative methods of degradation natural rubber. The widely used approach is degradation through the epoxidation reaction of isoprene units of the rubber polymer chain.…”

Section: Chemical Degradationmentioning

confidence: 99%

Degradation of non-vulcanized natural rubber renewable resource for fine chemicals used in polymer synthesis

Fainleib

Pires²,

Lucas³

et al. 2013

Polímeros

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In the current scenario, there is growing interest in the products of degradation of rubber (natural and synthetic) for specific applications in different industry sectors, whose benefits in replacing conventionally used products are mainly related to sustainability. Since the degradation products of rubber can be used in different areas, several research groups may have the interest aroused by these products, but are not familiar with the aspects related to the chemical behavior of rubber. This review aims to bring together the key information in the published literature on the degradation of natural rubber, emphasizing metatheses reactions, oxidative damage and splitting of the double bond, in order to serve as a reference source for researchers from different fields interested in obtaining such kind of products. The structures and properties as well as additional chemical transformations resulting in oligomers of isoprene, functionalised oligomers and polymers based on both are also described.

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Construction and intergeneric conjugative transfer of a pSG5-based cosmid vector fromEscherichia colito the polyisoprene rubber degrading strainMicromonospora aurantiacaW2b

Cited by 16 publications

References 19 publications

Identification and Characterization of Genes fromStreptomycessp. Strain K30 Responsible for Clear Zone Formation on Natural Rubber Latex and Poly(cis-1,4-isoprene) Rubber Degradation

Identification and Characterization of Genes fromStreptomycessp. Strain K30 Responsible for Clear Zone Formation on Natural Rubber Latex and Poly(cis-1,4-isoprene) Rubber Degradation

Biodegradation of Vulcanized SBR: A Comparison between Bacillus subtilis, Pseudomonas aeruginosa and Streptomyces sp

Degradation of non-vulcanized natural rubber renewable resource for fine chemicals used in polymer synthesis

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