“…We previously developed magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles, in order to improve accumulation of magnetite nanoparticles in target cells, due to electrostatic interaction between MCLs and the cell membrane (Shinkai et al, 1996). In addition, we recently developed a novel tissue engineering technique using MCLs, based on the fact that cells labeled with MCLs can be manipulated using magnetic force (Ito et al, 2004a(Ito et al, , 2004c(Ito et al, , 2005a(Ito et al, , 2005cShimizu et al, 2005). With this technique, we used a magnet to accumulate magnetically labeled keratinocytes onto a culture surface, and sheet-like multi-layered 3D constructs were successfully created without using any artificial polymer scaffolds (Ito et al, 2004a).…”