Construction and evaluation of Vibrio alginolyticus ΔclpP mutant, as a safe live attenuated vibriosis vaccine

Chen, Yanyan; Wu, Fenglei; Wang, Zhiwen; Tang, Jufen; Cai, Shuanghu; Jian, Jichang

doi:10.1016/j.fsi.2019.11.054

Cited by 17 publications

(11 citation statements)

References 32 publications

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“…tdh gene encodes thermostable direct hemolysin (TDH), which is a major virulence factor in V. alginolyticus (Avsever, 2016). Our previous research found that sodb and clpp genes are important virulence factors of V. alginolyticus, and deletion of those genes leads to reduction of bacterial virulence, suggesting they have potential application for the construction of live attenuated vaccines (Chen et al, 2019a;Chen et al, 2019b). Many virulence factors of V. alginolyticus were found to be succinylated in this study, indicating that lysine succinylation may play a crucial role in regulating the virulence of V. alginolyticus.…”

Section: Overlap Between Lysine Succinylation and Acetylation In V Alginolyticussupporting

confidence: 51%

First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence

Zeng

Pang

Chen

et al. 2021

Front. Cell. Infect. Microbiol.

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Recent studies have shown that a key strategy of many pathogens is to use post-translational modification (PTMs) to modulate host factors critical for infection. Lysine succinylation (Ksuc) is a major PTM widespread in prokaryotic and eukaryotic cells, and is associated with the regulation of numerous important cellular processes. Vibrio alginolyticus is a common pathogen that causes serious disease problems in aquaculture. Here we used the affinity enrichment method with LC-MS/MS to report the first identification of 2082 lysine succinylation sites on 671 proteins in V. alginolyticus, and compared this with the lysine acetylation of V. alginolyticus in our previous work. The Ksuc modification of SodB and PEPCK proteins were further validated by Co-immunoprecipitation combined with Western blotting. Bioinformatics analysis showed that the identified lysine succinylated proteins are involved in various biological processes and central metabolism pathways. Moreover, a total of 1,005 (25.4%) succinyl sites on 502 (37.3%) proteins were also found to be acetylated, which indicated that an extensive crosstalk between acetylation and succinylation in V. alginolyticus occurs, especially in three central metabolic pathways: glycolysis/gluconeogenesis, TCA cycle, and pyruvate metabolism. Furthermore, we found at least 50 (7.45%) succinylated virulence factors, including LuxS, Tdh, SodB, PEPCK, ClpP, and the Sec system to play an important role in bacterial virulence. Taken together, this systematic analysis provides a basis for further study on the pathophysiological role of lysine succinylation in V. alginolyticus and provides targets for the development of attenuated vaccines.

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Section: Overlap Between Lysine Succinylation and Acetylation In V Alginolyticussupporting

confidence: 51%

First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence

Zeng

Pang

Chen

et al. 2021

Front. Cell. Infect. Microbiol.

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“…However, there are now emerging alternative options in reducing disease burden in shrimp aquaculture system through vaccination (Milutinovic & Kurtz, 2016) and microbiome management with whole‐genome sequencing for health status management (Zhang et al, 2014) and CRISPR‐CAS editing system which are in the nascent stage of application in the aquaculture industry (Zhu & Ge, 2018). In recent years, it has been shown that even though shrimp does not have immunological memory, several antiviral and antimicrobial immune effort molecules and pathways like TLRs, IMD, JAK‐STAT, RNAi, P38, MAPK and JNK are present in shrimp (Musthaq & Kwang, 2014) and potential candidate vaccine for Vibrio infections in shrimp are in trials/commercialization viz., Vibromax™ vaccine (Chen et al, 2020; Kumaran et al, 2018; Powell et al, 2011).…”

Section: Measures Of Amu and Amr Mitigations In Shrimp Aquaculturementioning

confidence: 99%

Antimicrobial resistance in Vibrios of shrimp aquaculture: Incidence, identification schemes, drivers and mitigation measures

Vaiyapuri

Pailla

Badireddy

et al. 2021

Aquaculture Research

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Antimicrobial resistance (AMR) is a growing threat to the human, and animal population, worldwide. The antimicrobials used across all the sectors eventually pollute the aquatic environment and transform it into a hot spot for the emergence and spread of AMR. The bacteria under the genera Vibrio being ubiquitous to aquatic environment include a few species which are pathogenic to humans and aquatic animals. Despite extensive reviews available on AMR in human, terrestrial animal sectors and environment, there is a dearth of summarized information on AMR in Vibrios in shrimp aquaculture environment. In comparison to the human, and terrestrial animal health sector, a methodology for determination of AMR in vibrios associated with aquatic animals is still in its nascent stages and needs harmonization. The present review is centred on the AMR status in vibrios of shrimp farming environment and recent advances in the detection of resistance and discusses methodologies for AMR determination in vibrios and possible mitigation measures to control AMR spread.

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“…Some studies had shown that V. alginolyticus HY9901 inframe-deletion strains as live attenuated vaccines surely could improve specific and non-specific immunity and reduce mortality of pearl gentian groupers, such as DvscB, DacfA, and DclpP (Chen et al, 2019a;Chen et al, 2020;Pang et al, 2022). VscB as a T3SS chaperone protein, could assist the secretion or transport of the T3SS effector proteins and transported into host cells and participate in host intracellular functions (Pang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

A live attenuated strain of HY9901ΔdctP provides protection against Vibrio alginolyticus to the pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

Zhang

et al. 2022

Front. Mar. Sci.

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In recent decades, vibriosis caused by Vibrio alginolyticus has become a severe threat to the global mariculture industry. There is an urgent need for an effective vaccine to alleviate this unoptimistic situation. In this study, we evaluated the safety, immunoprotection, and specific and non-specific immune response effect of ΔdctP strain as a live-attenuated vaccine to pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatu). The results demonstrate that the safe dose of ΔdctP was ≤1.0 ×106 CFU in pearl gentian grouper. The relative percent survival of the pearl gentian grouper challenged with the ΔdctP mutant strain by intraperitoneal injection reached 74.4%, which was significantly higher than that of the control group. Meanwhile, the expression level of immune-relative genes, including IgM, IL-1β, IL-8, IL-10, MHC-Iα, MHC2, TNF-α, TLR3, and CD4, were upregulated in liver, spleen, and head kidney within 28 d post-vaccination. Moreover, specific antibody IgM, total serum protein as well as activities of catalase, superoxide dismutase, and lysozyme in serum were significantly up-regulated in vaccinated groupers compared with those in control. Collectively, ΔdctP could be used as a live-attenuated vaccine candidate against V. alginolyticus infection in pearl gentian grouper.

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Construction and evaluation of Vibrio alginolyticus ΔclpP mutant, as a safe live attenuated vibriosis vaccine

Cited by 17 publications

References 32 publications

First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence

First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence

Antimicrobial resistance in Vibrios of shrimp aquaculture: Incidence, identification schemes, drivers and mitigation measures

A live attenuated strain of HY9901ΔdctP provides protection against Vibrio alginolyticus to the pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

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