Construction and Evaluation of Urinary Bladder Bioreactor for Urologic Tissue-engineering Purposes

Davis, Niall F.; Mooney, Rory; Piterina, Anna V.; Callanan, Anthony; McGuire, Barry B.; Flood, Hugh D.; McGloughlin, Timothy M.

doi:10.1016/j.urology.2011.06.036

Cited by 26 publications

(18 citation statements)

References 20 publications

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“…Another possibility is that the utility of PDA for binding MnPNH 2 to the scaffold would be seen only at much lower concentrations of MnPNH 2 (less than the min imum concentration of 0.4 mM tested), where increased re tention aided by an adhesive would rise above saturation and The decellularized bladder, tracheal, and lung models used to exemplify our proposed labeling and monitoring approach were chosen because of their immediacy and rel evance in the field of tissue engineering. Research centers around the world are investigating ways using dECM mate rials to regenerate the bladder, 30 trachea, 7,31 and lungs, 32,33 amongst the many other tissue types not covered in this study. Because these efforts are largely in the infant stages of devel opment, where optimal cell types, for example, have yet to be uncovered, the application of our labeling method to study regeneration in vivo would yield the greatest insight when the regeneration approach itself has matured further to a stage where new tissue growth is confirmed but how to optimize that growth needs to be answered.…”

Section: Discussionmentioning

confidence: 99%

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

Szulc

Ahmadipour

Aoki

et al. 2019

Magnetic Resonance in Med

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Purpose To develop a facile method for labeling and imaging decellularized extracellular matrix (dECM) scaffolds intended for regenerating 3D tissues. Methods A small molecule manganese porphyrin, MnPNH2, was synthesized and used to label dECM scaffolds made from porcine bladder and trachea and murine whole lungs. The labeling protocol was optimized on bladder dECM, and imaging on a 3T clinical scanner was performed to assess reductions in T1 and T2 relaxation times. In vivo MRI was performed on dECM injected in the rat dorsum to verify sensitivity of detection. Toxicity assays for cell viability, metabolism, and proliferation were performed on human umbilical vein endothelial cells. The incorporation of MnPNH2 and its long‐term retention in dECM were assessed on transmission electron microscopy and ultraviolet absorbance of eluted MnPNH2 over time. Results All tissues, including thick whole 3D organs, were uniformly labeled and demonstrated high signal‐to‐noise on MRI. A nearly 10‐fold reduction in T1 was consistently obtained at a labeling dose of 0.4 mM, and even 0.2 mM provided sufficient contrast in vivo and ex vivo. No toxicity was observed up to 0.4 mM, the maximum tested. Binding studies suggested nonspecific association, and retention studies in the labeled whole decellularized lungs revealed less than 20% MnPNH2 loss over 30 days, the majority occurring in the first 3 days after labeling. Conclusion The proposed labeling method is the first report for visualizing dECM on MRI and has the potential for long‐term monitoring and optimization of dECM‐based organ tissue engineering.

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Section: Discussionmentioning

confidence: 99%

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

Szulc

Ahmadipour

Aoki

et al. 2019

Magnetic Resonance in Med

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“…Many different types of bioreactors such as spinner-flask, rotating wall, compression, strain, and flow perfusion bioreactors, have been under extensive investigation in tissue engineering research [6]. Combination of several bioreactor types has been preferably utilized as a novel way of cell culturing, this to construct functional tissue scaffolds [7]. Among various types of bioreactors, flow bioreactors can be regarded as one of the most promising designs, allowing efficient and uniform seeding of different cell types and ensuring an excellent cell viability due to a high diffusion rate of nutrients through the scaffolds [8,9].…”

Section: Introductionmentioning

confidence: 99%

Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System

Vardar

Engelhardt

Larsson

et al. 2015

Tissue Engineering Part A

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Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaffolds and specific cell culture techniques for urinary tract reconstruction. In this study, compressed tubular collagen scaffolds were evaluated, addressing the physical and biological characterization of acellular and cellular collagen tubes in a new flow bioreactor system, imitating the physiological pressure, peristalsis, and flow conditions of the human ureter. Collagen tubes, containing primary human smooth muscle and urothelial cells, were evaluated regarding their change in gene and protein expression under dynamic culture conditions. A maximum intraluminal pressure of 22.43 ± 0.2 cm H2O was observed in acellular tubes, resulting in a mean wall shear stress of 4 dynes/cm(2) in the tubular constructs. Dynamic conditions directed the differentiation of both cell types into their mature forms. This was confirmed by their gene expression of smooth muscle alpha-actin, smoothelin, collagen type I and III, elastin, laminin type 1 and 5, cytokeratin 8, and uroplakin 2. In addition, smooth muscle cell alignment predominantly perpendicular to the flow direction was observed, comparable to the cell orientation in native ureteral tissue. These results revealed that coculturing human smooth muscle and urothelial cells in compressed collagen tubes under human ureteral flow-mimicking conditions could lead to cell-engineered biomaterials that might ultimately be translated into clinical applications.

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“…Engineered, functionalized tissues have a high potential for various (bio-)medical applications [1,2,3,4]. Till now, there is a significant deficit in the generation of functionalized tissues due to the restricted and basic knowledge about suitable cell culture and mechanical training conditions.…”

Section: Introductionmentioning

confidence: 99%

“…To these belong mechanical stress, physiological co-cultivation of cells as well as suitable cell culture media. Literature shows that bioreactor and especially dynamic mechanical stresses are able to influence cellular proliferation and orientation [4,5]. For that reason, bioreactor technologies can be used to improve the knowledge about the in-vitro generation of tissues.…”

Section: Introductionmentioning

confidence: 99%

Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds

Seifarth

Goßmann²,

Janke³

et al. 2015

Urol Int

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Regenerative medicine, tissue engineering and biomedical research give hope to many patients who need bio-implants. Tissue engineering applications have already been developed based on bioreactors. Physiological ureter implants, however, do not still function sufficiently, as they represent tubular hollow structures with very specific cellular structures and alignments consisting of several cell types. The aim of this study was to a develop a new bioreactor system based on seamless, collagenous, tubular OPTIMAIX 3D prototype sponge as scaffold material for ex-vivo culturing of a tissue engineered ureter replacement for future urological applications. Particular emphasis was given to a great extent to mimic the physiological environment similar to the in vivo situation of a ureter. NIH-3T3 fibroblasts, C2C12, Urotsa and primary genitourinary tract cells were applied as co-cultures on the scaffold and the penetration of cells into the collagenous material was followed. By the end of this study, the bioreactor was functioning, physiological parameter as temperature and pH and the newly developed BIOREACTOR system is applicable to tubular scaffold materials with different lengths and diameters. The automatized incubation system worked reliably. The tubular OPTIMAIX 3D sponge was a suitable scaffold material for tissue engineering purposes and co-cultivation procedures.

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Construction and Evaluation of Urinary Bladder Bioreactor for Urologic Tissue-engineering Purposes

Cited by 26 publications

References 20 publications

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System

Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds

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