Construction and evaluation of the eukaryotic expression plasmid encoding two copies of somatostatin genes fused with hepatitis B surface antigen gene S

Liang, Aixin; Cao, Shulin; Han, Li; Yao, Yanfeng; Moaeen‐ud‐Din, Muhammad; Yang, Liguo

doi:10.1016/j.vaccine.2008.03.036

Cited by 24 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The copy number for the gene of interest within DNA construct is often increased to two or three copies to increase the amount of protein produced. 21,22 These highly similar regions can be potentially problematic for data analysis when using short-read technology, as short reads are generated from regions with a high prevalence of repeat sequences and cannot be de-convoluted. In comparison, tandemly repeated genes are not an issue for the long reads generated from the Pacific Biosciences system.…”

Section: Stability Of Transgenes In Complementing Cell Linesmentioning

confidence: 99%

New approaches for characterization of the genetic stability of vaccine cell lines

Gisonni-Lex

Azizi

2017

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

The genetic stability of cell lines is a critical analytical attribute required to demonstrate the quality of cells over time. During cell passage, mutations can arise in the genomic DNA, potentially leading to changes in the final vaccine product. The identity and integrity of master cell banks, extended cell banks, complementing cell lines or recombinant cell lines expressing transgenes has to be tested throughout the production process by the vaccine manufacturer. Over the past few years, the traditional methods for evaluation of genetic stability have been replaced with molecular approaches including quantitative PCR, digital PCR and high throughput sequencing. However, these molecular-based approaches are used in research laboratories and not within a GMP-compliant environment. In this article, we briefly discuss some opportunities and challenges in characterization of the genetic stability of vaccine cell lines with these molecular-based approaches.

show abstract

Section: Stability Of Transgenes In Complementing Cell Linesmentioning

confidence: 99%

New approaches for characterization of the genetic stability of vaccine cell lines

Gisonni-Lex

Azizi

2017

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

show abstract

“…As a third‐generation vaccine, DNA vaccines can elicit both humoral and cellular immune responses against extraneous genes (Pereira et al ., ). Compared with other vaccine technologies, the advantages of DNA vaccines include flexible genetic design, no risk of infection, high reagent stability and a relatively low production cost in the microbial host (Liang et al ., ). DNA vaccines have been widely used for various diseases, including avian influenza virus (Pan et al ., ) and HIV (Tuomela et al ., ).…”

Section: Introductionmentioning

confidence: 97%

The efficacy, biodistribution and safety of an inhibinDNAvaccine delivered by attenuatedSalmonella choleraesuis

Chen

Li²,

Xue

et al. 2017

Microbial Biotechnology

Self Cite

View full text Add to dashboard Cite

Summary DNA vaccines, the third‐generation vaccines, were extensively studied. The attenuated Salmonella choleraesuis (S. choleraesuis) was widely focused as a carrier to deliver DNA vaccines in the chromosome–plasmid balanced‐lethal system. The efficacy of inhibin DNA vaccine delivered by attenuated S. choleraesuis was proved in mice and cows in our previous studies. In this study, the efficacy of inhibin DNA vaccine was confirmed in rhesus monkeys. To further study the biodistribution and safety, the mice were immunized under laboratory conditions. The results of the rhesus monkeys showed the plasma IgA and IgG titres against inhibin were elevated, and the oestradiol (E2) and progesterone (P4) levels were increased with immunizing inhibin DNA vaccine. The biodistribution and safety assessment displayed the body weight, pathological change and haematology indexes where there is no significant difference between vaccinated mice and control. And the genomics analysis showed there was no integration of the inhibin gene into the mouse genome 2 months after immunization. This study indicated the inhibin DNA vaccine delivered by attenuated S. choleraesuis was safe. And this vaccine was a potential means to improve their reproductive traits in primates and other animals.

show abstract

“…HBsAg‐S gene as a large carrier molecule can effectively improve the immunogenicity of DNA vaccines encoding poor antigens (Liang et al. ; Gonzalez et al. ; Kotiw et al.…”

Section: Introductionmentioning

confidence: 99%

Hepatitis B Surface Antigen S Gene is an Effective Carrier Molecule for Developing GnRH DNA Immunocastration Vaccine in Mice

Han

Liu

et al. 2016

Reprod Domestic Animals

View full text Add to dashboard Cite

Relatively molecular mass of GnRH antigens is small and hence needs to couple to a large carrier molecule to enhance its immunogenicity. This study investigated whether hepatitis B surface antigen S (HBsAg-S) gene can be used as an effective carrier molecule for developing GnRH DNA immunocastration vaccine. Two copies of human GnRH gene were fused with HBsAg-S gene for constructing a recombinant plasmid pVAX-HBsAg-S-2GnRH that coded for 27 kDa target fusion protein. Ten male mice were divided into two equal groups, treatment and control. The vaccine (50 μg/mice) prepared in saline solution was injected into male mice at weeks 0, 1, 2, 4 and 7 of the experiment. Vaccine's efficacy was evaluated in terms of GnRH-specific IgG antibody response, plasma testosterone levels, testicular weight and extent of the testicular tissue damage. The specific anti-GnRH antibody titre in vaccinated animals was significantly higher than in controls in only 4th week of immunization (p < 0.05). In addition, vaccinated animals showed lower testicular weight than those of the controls (p < 0.05). Spermatogenesis in seminiferous tubules in vaccinated animals was suppressed. In conclusion, in this study, the engineered plasmid to be used as a GnRH DNA vaccine induced antibody response and suppressed spermatogenesis in mice. This suggests that HBsAg-S gene can be an effective carrier molecule for developing GnRH DNA immunocastration vaccine when relatively molecular mass of the aimed antigens is small.

show abstract

Construction and evaluation of the eukaryotic expression plasmid encoding two copies of somatostatin genes fused with hepatitis B surface antigen gene S

Cited by 24 publications

References 30 publications

New approaches for characterization of the genetic stability of vaccine cell lines

New approaches for characterization of the genetic stability of vaccine cell lines

The efficacy, biodistribution and safety of an inhibinDNAvaccine delivered by attenuatedSalmonella choleraesuis

Hepatitis B Surface Antigen S Gene is an Effective Carrier Molecule for Developing GnRH DNA Immunocastration Vaccine in Mice

Contact Info

Product

Resources

About