Construction and Evaluation of the Brucella Double Gene Knock-out Vaccine Strain MB6 Δbp26ΔwboA (RM6)

Abstract

Objective: Brucellosis is a serious zoonotic infection worldwide. To date, vaccination is the most effective measure against brucellosis. This study was aimed at obtaining a vaccine strain that has high protective efficacy and low toxicity, and allows vaccination to be differentiated from infection. Methods: Using homologous recombination, we constructed a double gene-deletion Brucella strain MB6 Δbp26ΔwboA (RM6) and evaluated its characteristics, safety and efficacy. Results: The RM6 strain had good proliferative ability and stable biological characteristics in vivo and in vitro. Moreover, it had a favorable safety profile and elicited specific immune responses in mice and sheep. Conclusion: The RM6 strain may have substantial practical application value.

Inflammatory Mechanism of Brucella Infection in Placental Trophoblast Cells

“…Brucellosis is a widespread chronic infectious disease caused by Brucella that infects humans and mammals. It has had a major impact on human life, health and social production since it was first recorded [ 1 , 2 ]. People are often infected with Brucella after eating animal products such as unpasteurized milk and meat or coming into direct contact with infected animals.…”