Construction and evaluation of replication-defective recombinant optimized triosephosphate isomerase adenoviral vaccination in Schistosoma japonicum challenged mice

Dai, Yang; Wang, Xiaoting; Song, Zhao; Tang, Jianxia; Zhang, Lu; Dai, Jianrong; Zeng, Mingtao; Lu, S. C.; Zhu, Yinchang; Su, Chuan

doi:10.1016/j.vaccine.2013.12.059

Cited by 25 publications

(25 citation statements)

References 61 publications

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“…Moreover, we observed that vaccination seems to reduce the eggs trapped in tissues per female in hamsters pointing an impairment of the oviposition capacity of the surviving females as happens in repetitive natural infections (Vercruysse and Gabriel, 2005). The anti-fecundity effect along with reduction of egg viability is considered useful for reduction transmission in schistosomiasis (Ahmad et al, 2009;Dai et al, 2014). Furthermore, a significant increase of IgG2a against SoSbAWA was observed in protected mice in vaccination experiment.…”

Section: Discussionmentioning

confidence: 82%

The combination of the aliphatic diamine AA0029 in ADAD vaccination system with a recombinant fatty acid binding protein could be a good alternative for the animal schistosomiasis control

Vicente

López‐Abán

Rojas-Caraballo

et al. 2015

Experimental Parasitology

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Section: Discussionmentioning

confidence: 82%

The combination of the aliphatic diamine AA0029 in ADAD vaccination system with a recombinant fatty acid binding protein could be a good alternative for the animal schistosomiasis control

Vicente

López‐Abán

Rojas-Caraballo

et al. 2015

Experimental Parasitology

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“…Schistosoma japonicum eggs were provided by Chuan Su (Nanjing Medical University, Nanjing, China) (33). For the IL-4R-driven lung granuloma model, mice were primed with an i.p.…”

Section: Schistosomiasis Modelmentioning

confidence: 99%

Myeloid-Specific Disruption of Tyrosine Phosphatase Shp2 Promotes Alternative Activation of Macrophages and Predisposes Mice to Pulmonary Fibrosis

Tao

Jin

et al. 2014

The Journal of Immunology

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The alternative activation of M2 macrophages in the lungs has been implicated as a causative agent in pulmonary fibrosis; however, the mechanisms underlying M2 polarization are poorly characterized. In this study, we investigated the role of the ubiquitously expressed Src homology domain–containing tyrosine phosphatase Shp2 in this process. Shp2 inactivation augmented IL-4–mediated M2 polarization in vitro, suggesting that Shp2 regulates macrophage skewing and prevents a bias toward the M2 phenotype. Conditional removal of Shp2 in monocytes/macrophages with lysozyme M promoter–driven Cre recombinase caused an IL-4–mediated shift toward M2 polarization. Additionally, an increase in arginase activity was detected in Shp2∆/∆ mice after i.p. injection of chitin, whereas Shp2-deficient macrophages showed enhanced M2 polarization and protection against schistosome egg–induced schistosomiasis. Furthermore, mutants were more sensitive than control mice to bleomycin-induced inflammation and pulmonary fibrosis. Shp2 was associated with IL-4Rα and inhibited JAK1/STAT6 signaling through its phosphatase activity; loss of Shp2 promoted the association of JAK1 with IL-4Rα, which enhanced IL-4–mediated JAK1/STAT6 activation that resulted in M2 skewing. Taken together, these findings define a role for Shp2 in alveolar macrophages and reveal that Shp2 is required to inhibit the progression of M2-associated pulmonary fibrosis.

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“…SjTPI, a potential vaccine candidate, has been studied for many years [ 24 , 25 ]. In our previous study, we constructed a replication-defective, adenoviral-based vaccine encoding the optimized SjTPI gene (rAdV-SjTPI.opt) which, when injected intramuscularly into mice, could elicit a high level of specific Th1, IgG responses and a 50% worm reduction rate [ 26 ]. To further improve the protective efficacy induced by rAdV-SjTPI.opt, in the present study, we immunized mice with the rAdV-SjTPI.opt intramuscular priming and rSjTPI protein subcutaneous boosting strategy, tested the specific immune responses, and evaluated the protective efficacy through challenge infection of Schistosoma japonicum with cercariae.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Protective Efficacy through Adenoviral Vectored Vaccine Priming and Protein Boosting Strategy Encoding Triosephosphate Isomerase (SjTPI) against Schistosoma japonicum in Mice

et al. 2015

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BackgroundSchistosomiasis japonica is a zoonotic parasitic disease; developing transmission blocking veterinary vaccines are urgently needed for the prevention and control of schistosomiasis in China. Heterologous prime-boost strategy, a novel vaccination approach, is more effective in enhancing vaccine efficacy against multiple pathogens. In the present study, we established a novel heterologous prime-boost vaccination strategy, the rAdV-SjTPI.opt intramuscular priming and rSjTPI subcutaneous boosting strategy, and evaluated its protective efficacy against Schistosoma japonicum in mice.Methodology/Principal FindingsAdenoviral vectored vaccine (rAdV-SjTPI.opt) and recombinant protein vaccine (rSjTPI) were prepared and used in different combinations as vaccines in a mouse model. The specific immune responses and protective efficacies were evaluated. Furthermore, the longevity of protective efficacy was also determined. Results showed that the rAdV-SjTPI.opt priming-rSjTPI boosting strategy elicited higher levels of specific IgG responses and broad-spectrum specific cellular immune responses. The protective efficacy could reach up to nearly 70% and 50% of protection could be observed at 10 weeks after the last immunization in mice.Conclusions/SignificanceThe rAdV-SjTPI.opt intramuscular priming-rSjTPI subcutaneous boosting vaccination strategy is a novel, highly efficient, and stable approach to developing vaccines against Schistosoma japonicum infections in China.

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Construction and evaluation of replication-defective recombinant optimized triosephosphate isomerase adenoviral vaccination in Schistosoma japonicum challenged mice

Cited by 25 publications

References 61 publications

The combination of the aliphatic diamine AA0029 in ADAD vaccination system with a recombinant fatty acid binding protein could be a good alternative for the animal schistosomiasis control

The combination of the aliphatic diamine AA0029 in ADAD vaccination system with a recombinant fatty acid binding protein could be a good alternative for the animal schistosomiasis control

Myeloid-Specific Disruption of Tyrosine Phosphatase Shp2 Promotes Alternative Activation of Macrophages and Predisposes Mice to Pulmonary Fibrosis

Enhancement of Protective Efficacy through Adenoviral Vectored Vaccine Priming and Protein Boosting Strategy Encoding Triosephosphate Isomerase (SjTPI) against Schistosoma japonicum in Mice

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