Construction and evaluation of nitric oxide generating vascular graft material loaded with organoselenium catalyst via layer-by-layer self-assembly

Abstract

A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide (NO) was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun polycaprolactone matrix via electrostatic layer-by-layer self-assembly. This material revealed significant NO generation when contacting NO donor S-nitrosoglutathione (GSNO). Adhesion and spreading of smooth muscle cells were inhibited on this material in the presence of GSNO, while proliferation of endothelial cells was promoted. In vitro platelet adhesion and arteriovenous shunt experiments demonstrated good antithrombotic properties of this material, with inhibited platelet activation and aggregation, and prevention of acute thrombosis. This study may provide a new method of improving cellular function and antithrombotic property of vascular grafts. nitric oxide, catalytic generation, electrospinning, vascular graft, biocompatibility Citation:An J, Chen SY, Gao JC, Zhang X, Wang YY, Li YD, Mikhalovsky S, Kong DL, Wang SF. Construction and evaluation of nitric oxide generating vascular graft material loaded with organoselenium catalyst via layer-by-layer self-assembly. Sci China Life Sci, 2015, 58: 765 -772,

Nitric oxide-generatingl-cysteine-grafted graphene film as a blood-contacting biomaterial

“…[1][2][3][4][5] Among them, due to the profiles of anti-platelet aggregation activity and regulation of vasodilation, blood-contacting biomaterials (such as vascular grafts, stents, extracorporeal circuits, and heart valves), which are expected to be capable of creating a local NO release at the blood-material interface, have drawn considerable attention for mitigating the risk of thrombus formation. [6][7][8] Incorporation of exogenous NO donors on prosthetic materials, such as silicone rubber 9 and polymethacrylate, 10 was by far a popular route to fabricate blood-contacting biomaterials. However, most exogenous NO donors are usually unstable under some physiological conditions such as temperature, pH, and ion strength, thus causing the burst release of NO in water and physiological environments (that is, blood, body fluids, and so on).…”