Construction and Evaluation of a Humic Acid Column: Implication for Pesticide Risk Assessment

André, Carl; Truong, Tu T.; Jf, Robert; Thomassin, Mireille; Yc, Guillaume

doi:10.1021/ac050005k

Cited by 16 publications

(5 citation statements)

References 31 publications

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“…The temperature dependence of the nor-NOHA retention factor is given by the well known thermodynamic relation [57,58]:…”

Section: Possible Thermodynamic Origins Of the Nor-noha Binding To Armentioning

confidence: 99%

Immobilization of arginase and its application in an enzymatic chromatographic column: Thermodynamic studies of nor-NOHA/arginase binding and role of the reactive histidine residue

Bagnost

Guillaume

Thomassin

et al. 2007

Journal of Chromatography B

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“…The temperature dependence of the nor-NOHA retention factor is given by the well known thermodynamic relation [57,58]:…”

Section: Possible Thermodynamic Origins Of the Nor-noha Binding To Armentioning

confidence: 99%

Immobilization of arginase and its application in an enzymatic chromatographic column: Thermodynamic studies of nor-NOHA/arginase binding and role of the reactive histidine residue

Bagnost

Guillaume

Thomassin

et al. 2007

Journal of Chromatography B

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“…[25][26][27][28][29]. These stationary phases were also used for the binding study of pesticides and rodenticides [30,31] or metal ions present in soil and water with HAs [32]. When the HA stationary was prepared, it filled an HPLC chromatographic column, with most of the time, an i.d.…”

Section: Introductionmentioning

confidence: 99%

New liquid chromatography columns for highlighting the interaction of ligand candidates with humic acid

Guillaume

André²

2023

J of Separation Science

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Humic acid was the main compound in soil and reduced the availability of some organic compounds in soils. In this work, humic acid was immobilized for the first time on a homemade neutravidin poly(glycidyl methacrylate-co-ethylene dimethacrylate) capillary column with a 20 μm i.d. for the screening of potential ligands to humic acid and the evaluation of their molecular recognition mechanism. This homemade humic acid column enabling it to work at very low backpressure (0.60 MPa at 20 nl/min flow rate), had a long lifetime, excellent repeatability, and negligible non-specific binding sites. The performance of this affinity humic acid column was demonstrated by the evaluation of recognition assay for a series of known ligands of humic acid (a series of rodenticide molecules) which is the heart of the fragment-based drug design. In addition, this column was used successfully for highlighting the binding mechanism to humic acid of the severe acute respiratory syndrome coronavirus-2-spike protein.As well this new humic acid miniaturized liquid chromatography column developed in this work could be used in the feature for another solute molecule-humic acid binding studies or for a separative mode.

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“…However, pesticide residues have induced increasing attention due to their threat to life health and environment. Studies show that chronic contact pesticides might lead to adverse effects on the consumers [3][4][5]. Therefore, the detection of pesticide residues is extremely urgent.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of pesticide residues in fruits by surface-enhanced Raman scattering based on modified QuEChERS pretreatment method with portable Raman instrument

Jiang

Liu

et al. 2019

SN Appl. Sci.

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In this work, surface-enhanced Raman scattering (SERS) spectrum was used to build a rapid analytical method for pesticide residues in fruits. QuEChERS (quick, easy, cheap, effective, rugged and safe) method was modified by multi-walled carbon nanotubes as clean-up sorbents. Overall recoveries of selected pesticide phosmet ranged from 77 to 97% in apples at three spiking levels (0.5, 1 and 2 mg kg −1). The relative standard deviations were between 6.6 and 14%. The limit of detection for phosmet was 0.1 mg kg −1 in standard solution and 0.5 mg kg −1 in apples, which was below the maximum residue limits in fruits of USA, EU and China. The intensity of phosmet characteristic peak existed good linear relationship with the logarithm of concentration between 0.5 and 5 mg kg −1 , with the calibration curve coefficients (R 2) of 0.9994, which indicated quantitative potential for pesticide residue detection. The method was extended to other pesticides, and the obtained SERS results could be used to establish a spectra database. All the experiments were performed with a portable Raman instrument. Combining pretreatment method with spectra database, a sensitive, rapid and convenient method could be built for pesticide residues detection in fruits.

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Construction and Evaluation of a Humic Acid Column: Implication for Pesticide Risk Assessment

Cited by 16 publications

References 31 publications

Immobilization of arginase and its application in an enzymatic chromatographic column: Thermodynamic studies of nor-NOHA/arginase binding and role of the reactive histidine residue

Immobilization of arginase and its application in an enzymatic chromatographic column: Thermodynamic studies of nor-NOHA/arginase binding and role of the reactive histidine residue

New liquid chromatography columns for highlighting the interaction of ligand candidates with humic acid

Rapid detection of pesticide residues in fruits by surface-enhanced Raman scattering based on modified QuEChERS pretreatment method with portable Raman instrument

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